Core-Shell

Characteristics of SunShell C18Particle size(µm)Core shell silicaPore diameter(nm)Specific surface area(m 2 /g)Carbon content(%)BondedphaseC18Maximum operatingpressureAvailable pHrangeSunShell C18 2.6 9 150 7 C18 60 MPa or 8,570 psi 1.5 - 10Comparison of standard samples between core shell C18s23123145647Brand D C18 (Core shell)756Brand E C18 (Core shell)Column:Brand D C18, 2.6 µm 150 x 4.6 mm (Core shell type)Brand E C18, 2.7 µm 150 x 4.6 mm (Core shell type)SunShell C18, 2.6 µm 150 x 4.6 mmMobile phase: CH 3 OH/H 2 O=75/25Flow rate: 1.0 mL/minTemperature: 40 º CSample: 1 = Uracil, 2 = Caffeine, 3 = Phenol, 4 = Butylbenzene5 = o-Terphenyl, 6 = Amylbenzene, 7 = Triphenylene213SunShell C18 (core shell)4567Hydrogen bonding(Caffeine/Phenol)Hydrophobicity(Amylbenzene/Butylbenzene)Steric selectivity(Triphenylene/o-Terphenyl)Brand D C18 0.48 1.54 1.20Brand E C18 0.44 1.60 1.310 2 4 6 8 10 12 14 16 18 20 22 24Retention time/minSunShell C18 0.40 1.59 1.47Retention of standard samples was compared for three kinds of core shell type C18s. Brand D C18 showed only a half retention tocompare with SunShell C18. Steric selectivity becomes large when ligand density on the surface is high. SunShell C18 has thelargest steric selectivity so that it has the highest ligand density. This leads the longest retention time.Comparison of pyridine Comparison of Oxine Comparison of formic acidBrand D C18(Core shell)123Brand D C18(Core shell)12123Brand D C18(Core shell)Brand E C18(Core shell)123Brand E C18(Core shell)12123Brand E C18(Core shell)SunShell C18(Core Shell)12 3SunShell C18(Core shell)12123SunShell C18(Core shell)0 1 2 3 4 5 6 7Retention time/minColumn:Brand D C18, 2.6 µm 150 x 4.6 mmBrand E C18, 2.7 µm 150 x 4.6 mmSunShell C18, 2.6 µm 150 x 4.6 mmMobile phase: CH 3 OH/H 2 O=30/70Flow rate: 1.0 mL/minTemperature: 40 ºCDetection: UV@250nmSample: 1 = Uracil2 = Pyridine3 = Phenol8 9 10 11 12 130 1 2 3 4 5 6Retention time/minColumn:Brand D C18, 2.6 µm 150 x 4.6 mmBrand E C18, 2.7 µm 150 x 4.6 mmSunShell C18, 2.6 µm 150 x 4.6 mmMobile phase: CH 3 CN/20mM H 3 PO 4 =10/90Flow rate: 1.0 mL/minTemperature: 40 ºCDetection: UV@250nmSample: 1 = 8-Quinolinol (Oxine)2 = Caffeine0 1 2 3 4 5 6Retention time/min7 8 9 10Column:Brand D C18, 2.6 µm 150 x 4.6 mmBrand E C18, 2.7 µm 150 x 4.6 mmSunShell C18, 2.6 µm 150 x 4.6 mmMobile phase: CH 3 CN/0.1% H 3 PO 4 =2/98Flow rate: 1.0 mL/minTemperature: 40 ºCDetection: UV@210nmSample: 1 = Formic acid2 = Acetic acid3 = Propionic AcidResidual silanol groups make pyridine betailing under methanol/water mobile phasecondition. Only SunShell C18 shows asharp peak for pyridine.8-Quinolinol (Oxine) is a metal chelatingcompound. Metal impurities in the coreshell particle leads the tailing for oxinepeak.Formic acid is used as an indicator for aacidic inertness. SunShell and brand DC18 show a sharp peak.Website : www.chromtech.net.au E-mail : info@chromtech.net.au TelNo : 03 9762 2034 . . . in AUSTRALIA