LC-2010HT

C196-E056CLC-2010HTShimadzuHigh-performance Liquid Chromatograph

High Throughput HPLCLC-2010HTWe HAVE it all !

LC-2010HTShimadzu High-performance Liquid ChromatographAdvanced total solutions lead the way to the new generation.HPLC is currently one of the primary tools used in a wide variety of fields including pharmaceuticals,food products, general chemistry, and environmental analysis. The increasing demand for even higherthroughput analysis is emerging due to the strong influence of the pharmaceutical industry, which hasbeen stimulated by new drug discovery strategies designed to promote more efficient productdevelopment and globalization. The environments that chromatographs are used in are changinggreatly, as there is a constant demand for improved compliance to legal regulations represented inGood Laboratory Practice (GLP) and Good Manufacturing Practice (GMP) guidelines, and newcompliance issues raised by the Food and Drug Administration [FDA]. Here, the conventional workingprocedures used by analysts to extract data require special knowledge and experience, making it achallenge to meet the needs of a finely delineated and speed-oriented information society.Blinding fast high-throughput analysisEffective automation of your analysisAssured data reliability and validationOperating system that anyone can easily useHigh ThroughputAutomationValidationEase of UseandSuperb performance realizingnear-zero sample carryoverA new look, new concept HPLCShimadzu presents you with the LC-2010HT and LCsolution.The new look and total solution for your current and future HPLC needs.LC-2010HTTheLC-2010HT is a next-generation HPLC based on the concept of high-throughput analysis andautomated validation. The LC-2010HT is comprised of a degassing unit, low-pressure gradient unit,pump unit, mixer, ultra fast autosampler, column oven, and a UV-VIS detector with a thermostattedflow cell. System reliability has been further improved by standardizing the flow line arrangement inorder to integrate the units. New and improved features include flow rate accuracy, gradientconcentration accuracy, and reduction of equipment flow path volume differences, and near zerosample carryover. The LC-2010HT is available in two models, the standard model (LC-2010HT) andthe sample-cooled model (LC-2010HT), for use across a broad range of applications from conventionalliquid chromatography to semi-micro liquid chromatography.ContentsP 04 -Research developmentacceleration!P 10 -Intelligent HPLC enablesrapid mastery!P 16 - SpecificationsP 06 -Automated Analysis andLabor Saving!P 12 -Superior performance anddurability!P 18 - OptionsP 08 - Easy Validation realized!P 14 -Presenting the latest datamanagement environment!

Research development acceleration!High throughputThe latest technology for multiple samples, high-speed injection andautomation is now condensed into a compact package!This is optimum next-generation HPLC for high-throughput analysis.High throughput is in much demand in many facets of industry such as research, development,manufacturing, and product quality control. The Shimadzu LC-2010HT is a new generation highthroughputHPLC developed to meet this demand.High ThroughputAutosamplerThe high-performance metering pumpcan precisely measure samplevolume with no sample loss.In addition to superior repeatability(within 0.3%RSD* 1 ) and low carryover(within 0.01%* 1 ), the capacity andspeed are greatly improved with thisHigh Throughput Autosampler. Twosample racks for 1.5mL vials areequipped as standard.Reservoir TrayThe Reservoir Tray canaccommodate six 1L bottles.Documents and small tools can bestored in the drawer, too.System ControllerThe Graphical User Interface (GUI)and Wizard function make it easy tooperate the LC-2010HT.Sample Cooler(LC-2010CHT)* 2A direct cooling mechanism withPeltier elements* 2 provides forefficient and rapid cooling. The newlydesigned dehumidifier preventscondensation.DegasserA small-volume, high-efficiency 5-linedegasser is incorporated to removedissolved gases in mobile phasesand in the rinsing solution of theautosampler.PumpPulse-free solvent delivery is achieved by utilizingShimadzu's proprietary micro volume serial doubleplunger design. A newly developed Dynamic InletValve (Pat. Pending) greatly improves flow ratestability and gradient performance.Column OvenThe block heating-type column ovencan heat and cool up to two columns.The temperature deviation isminimized by a double temperaturecontrol mechanism.The advanced Column ManagementDevice (CMD)* 3 can be used toeasily manage column information.UV-VIS DetectorThanks to the lowest baseline noise(±0.25 x 10 -5 AU or lower* 1 ) ever,higher sensitivity analyses areachieved. The standard temperaturecontrolledcell reduces the influenceof room temperature fluctuations onabsorbance and gives a more stablebaseline. A mercury lamp is built in tocheck wavelength accuracy.*1 Under the analytical conditions determined by Shimadzu*2 Standard for the LC-2010HT*3 Optional item4

Large sample capacityThe LC-2010HT can handle up to 350 1mL vials or 210 1.5mL vials* 1 .When microtiter plate racks are used, up to 4 microtiter plates can beaccommodated for continuous analysis of max. 1,536 samples.* 2Deep Well plates can also be used. Two separate sample racks are used so thatyou can load samples in a rack while analyses are performed on samples fromthe other rack.*1 LC-2010AHT*2 When 384-well plates are used.High-speed injectionInjection time per sample has been greatly reduced. Just 15 seconds* arerequired from start to finish for unheard of high-speed sample injection.*For a 10μL injection volumeHigh-speed analysisSeven analyses are completed in 15 minutes thanks to high-speed injection and anultra-fast column!This enables major improvements in analysis throughput in the field of drugdiscovery.The desired data can be retrieved quickly bynarrowing the search areaDatabaseMultiple data are managed in one block with optionalCLASS-Agent Manager*.Required data can be easily retrieved from a mass ofdata as analysis data is automatically loaded into thedatabase.*Standard component of optional CLASS-Agent software.Display of the target data5

Automatic recognitionAutomatically recognizes the sample racktype and detects vials, flow cell type, solventleak, lamp house cover status and oven doorstatus, etc.Automation functionsThe LC-2010HT is replete with sensibleautomation functions to lighten yourworkload.Auto system checkAutomatic check of baseline stability andsystem status.Auto validationAutomated high-level validation can beperformed quickly, eliminatingtime-consuming periodic manual validations.Auto logMaintenance log, operation log, error log aswell as column history can be automaticallyrecorded. ( * )* CMD option required.Parameter setting screen for mobile phase and rinse solutionAuto purge functionsThe drain valve no longer has to be opened and closedmanually as with conventional pumps. One touchautomatically purges each flow line and rinse liquid linefor the autosampler.You can even be warned that theremaining mobile phase is less thanthe set level.7

Easy Validation realized!ValidationAnyone can promptly perform high-level validation at any time!Supports GLP and GMP to an even greater degree.High levels of validation in every field are required for analytical instruments that have to complywith regulations such as GLP, GMP, and ISO. The LC-2010HT is equipped with an auto-validationfunction to enable high-level validation of hardware.Used with LCsolution and CLASS-Agent manager, results of validation are saved as electronicrecords.Auto validationPreviously, knowledge and experiencewere prerequisites for executing validationtests, but now anyone can performvalidations using a uniform procedure.The LC-2010HT auto-validation functionautomatically finishes in approximately 1.5hours in isocratic mode and approximately3 hours in gradient mode.Validation executionValidation startValidation finishAuto validation tests important parameters such as solvent deliverystability, wavelength accuracy, absorbance level, and column temperature.Performance checkThe performance-check function can be used if the validationof each unit is to be performed independently. This is a usefulfunction when validations such as accuracy of the pump flowrate and injection volume accuracy need to be performedindividually. This performance check can be executed even ifthe instruction manual is not available, since the validationprocedure instructions are displayed on screen.Performance check screen8

System checkDaily checks can be performed automaticallywith the system check function. Device statuscan be easily checked as auto validation andperformance check functions record the latestvalidation results together with deviceself-diagnosis results and the remaining servicelife status of consumables.System check screenSystem check reportSystem suitability testLCsolution is equipped with a QA/QC function as standard so thatsystem suitability tests can be easily performed on a daily basis. Also,it is possible to automate the operations that occur in a routineanalysis, such as re-injections and analysis cancellations that dependon test results if the auto-validation function for results is used.QA/QC reportIQ: installation QualificationOQ: Operational QualificationIQOQOQFDA compliance supportWhen devices are installed or reinstalled due to amove, checks must be carried out to ensure that theinstallation environment and device meet thespecifications and the device performance meets theestablished criteria in order for the instrument tocomply with FDA inspections. In the case ofinstallation qualification (IQ) checks, analysis devicespecifications, software, attached documents, andsuitability qualification at the time of deviceinstallation (including installation environment checkas well as customer training certificate) are required.With the LC-2010HT, installation qualification andoperational qualification (OQ) documents areattached as standard. Shimadzu qualified servicepersonnel perform installation qualification andoperational qualification (service cost is not included).9

Intelligent HPLC enables rapid mastery!Ease of useAutomation functions and an analysis application CD-ROM providepowerful support during daily analysis!All consumables can be replaced from the front of the instrument.Mastery of equipment operating method….Here, valuable time is saved - which minimizes the total cost - as the LC-2010HT is an intelligentHPLC fully equipped with the necessary functions and features for daily operation and maintenance.Graphical User Interface (GUI)Analytical conditions can be set intuitively using GUIscreens. Even beginners can operate it very easily.Easy Schedule SettingAn analysis schedule can be prepared easily from theinjection sequence window.10

Automatic recording of column historyWhen the CMD is used, the injection number,eluent volume, and the previously used mobilephase, etc., are automatically recorded, so thecolumn history can be easily checked.Screen for settingcolumn dataA column with CMDAnalysis history can be checked.Front access for maintenanceMaintenance such as lamp or flow cellreplacement is easy.Cell replacementLamp replacementAnalysis application CD-ROMAnalysis is possible as soon as the sample is prepared.Installation only requires preparation of the mobile phaseand column; therefore, complicated plumbing and wiring arenot needed. Moreover, anyone can easily use the analysisapplication CD-ROM because it is complete with analyticalknow-how such as analysis parameters and the mobilephase preparation method.Screen of analysis applicationCD-ROM11

Superior performance and durability!High performanceThis new instrument configuration redefines the benchmarks forperformance and reliability.Gradient performanceGradient concentration accuracy is further improvedthrough the use of a newly developed dynamic inletvalve (patent pending).Excellent results are obtained with the LC-2010HT,even in the area of lab-to-lab reproducibility that isoften an issue in validation tests.Set0369121520304050Actual Error (%) Set Actual0.00 0.006059.813.03 0.037069.875.96 -0.048079.878.98 -0.028584.8611.87 -0.138887.8815.00 0.009190.8019.93 -0.079493.8529.86 -0.149796.8539.87 -0.13 100 100.0049.84 -0.16Example of actual measured concentrationError (%)-0.19-0.13-0.20-0.14-0.12-0.20-0.15-0.150.00Injection volume accuracyImprovements to the drive units of the autosamplerhave simultaneously achieved greaterspeed, higher reproducibility, and superiorreliability.Even micro-volume sample injections can beperformed accurately. All of the sample drawn isinjected, so there is no sample loss, andinjection volume linearity is excellent.Peak areaInjection volume linearityLC-2010HT Injection volume linearityInjection volume accuracyInjection volumeSet (μL) Actual (μL)5050.081010.0755.0811.05Note:Injection volume accuracy ismeasured using thegravimetric method.Injection volume (μL)Near-zero sample carryoverThe surface of the sampling needle of the LC-2010HT’sautosampler is coated using a special, innovativesurface processing technology (patent pending).Furthermore, by employing newly developed rotor sealsand needle seals made of PEEK, the LC-2010HTsignificantly reduces contamination, even with the mosthighly adsorbent sample compounds. As a result, theLC-2010HT demonstrates nearly zero sample carryover.The use of PEEK for the rotor seals means that theLC-2010HT has strong durability (5 times strongercompared to previous models).12

Set No.12345678910AVG50μL 10μL 5μL0.06% 0.18% 0.06%0.07% 0.18% 0.25%0.05% 0.03% 0.03%0.06% 0.07% 0.09%0.07% 0.05% 0.15%0.03% 0.16% 0.14%0.05% 0.04% 0.20%0.05% 0.18% 0.17%0.03% 0.04% 0.16%0.05% 0.18% 0.08%0.05% 0.11% 0.13%Repeatability data injection volume1μL0.69%0.45%0.57%0.74%0.49%0.76%0.65%0.59%0.93%0.83%0.67%Injection volume repeatability and stabilityStability is another important factor that goes hand-in-handwith achieving high-speed injection to support high-throughputanalysis.Also, the main components such as the measuring pump,injection port, and valve are easily accessible from theinstrument's front panel.This example shows the reproducibility of data for injectionvolumes. Each injection volume was measured 50 times andthe RSD (%) of the area value was determined.The LC-2010HT provides extremely stable data.Actual value (AU)LC-2010HT Detector linearityDetector linearityDetector linearity has been furtherextended through the innovativedevelopment of a highly reliableoptical system.A temperature-controlled flow cellimproves baseline stability in analysesusing mobile phases that experienceabsorbance variation with temperaturechanges.Set value (AU)Temperature control flow cellArea 43604276Sample carryover forchlorhexidine, which stronglyabsorbs to a samplingneedle with conventionalmaterial, is only0.0014%Area 616Sample carryover (2μL injection)13

Presenting the latest data management environment!NetworkNetwork capability and 21 CFR Part 11 complianceShimadzu's basic policy is to comply with 21 CFR Part 11 requirements by integrating datamanagement for all instruments used in the laboratory, including chromatographs and massspectrometers (HPLC, GC, LCMS, GCMS), spectrophotometers (UV, FTIR etc.), TOC analyzers,thermal analyzers, and balances.CLASS-Agent Network SystemShimadzu's CLASS-Agent network system provides a solution for 21 CFR Part11 compliance of all essentiallaboratory analysis data from chromatographs and spectrophotometers to balances.LABORATORYLCGCLCMSGCMSTOCCLASS-Agent Network SystemSeverIntegratedManagement ofAnalytical DataUV FTIR AA Balances ThermalAnalyzersOther vendor’sinstrumentsData search,re-analysis andapprovalIntegrated data managementCLASS-Agent Manager is a browsingsoftware for analytical data registered in thedatabase. With CLASS-Agent Manager,data browsing, inspection, and approval ofdata for chromatography andspectrophotometry and balances can beachieved without the need to learn newoperations for the workstation or theanalytical instrument's software.14

Integration with CLASS-AgentIntegration of LCsolution with the CLASS-Agent databasesystem has been made more seamless by placing the databaseregistration tool inside the LCsolution package.Electronic Signature CapabilityConversion to paperless operations brings various advantagesfrom reduction of paper consumption to reduction of storagespace. The CLASS-Agent Manager has an electronic signaturecapability, which makes full conversion to the paperlessoperation possible.CLASS-Agent ReportCLASS-Agent Report registers pre-createdtemplates to a database. When a report isgenerated, it automatically selects thetemplate, pulls required data from thedatabase and conducts the calculations.Through this procedure, only templates anddata already in the database are used forcalculations. The Excel spreadsheet thatcalculated the results is also saved andmanaged in the database. Consequently,the user does not directly touch the data,thereby maintaining the audit trail, security,and data integrity.1. Excel template preparationAgentDatabase2. Data base registration Excel templatesAutomatic registrationAnalysis resultExcel ReportData from analytical instrumentsPDF Report3. Template and data selectionBackground processing4. Data insertion to templateand PDF generation5. Automatic entry to database15

SpecificationsSpecificationsSpecifications of LC-2010HTItem LC-2010HT (228-45103-3X) LC-2010HT (228-45102-3X)PumpPump typeFlow rate setting rangeFlow rate accuracyFlow rate precisionPressure display accuracyPlunger cleaningSerial dual pluger, micro volume (10μL on primary side, 5μL on secondary)0.001-5 mL / min [1.0-35 Mpa / 145-5076psi]±1% or ±2μL / min, whichever is larger (1mL / min, 3-10.0MPa / 435-1450psi, water, ambient temperatureat 20°C, except in gradient solvent delivery), ±2% or ± 2μL / min, whichever is larger(except above condition and in gradient solvent delivery)±0.075% RSD or 0.02min SD, whichever is larger (0.05-2mL / min, 1.0-35Mpa / 145-5076psi, methanol,ambient temperature at 20°C)±2% or ±0.5MPa, whichever is largerAutomatic rinsing mechanism as standard equipmentLPGE Number of solvents andsetting rangeConcentration accuracyConcentration precisionUp to 4 solvents0-100%, 0.1% step±0.5%(0.1-2mL / min, 1.0-20.0MPa / 145-2900psi, aqueous acetone / water)±0.1%(0.1-2mL / min, 1.0-20.0MPa / 145-2900psi, aqueous acetone / water)DegasserAutosamplerTypeVolumeInjection methodInjection volume setting rangeMembrane on-line degasser, 5 lines (4x mobile phase, 1x autosampler rinsing liquid)4mL / line for mobile phase, 2mL / line for autosampler rinsing liquidFull volume injection, variable injection possible (no sample loss)0.1-100μL (standard), 1-2000μL (optional)(0.1-0.9μL in 0.1μL step, 1-2000μL in 1μL step)Samplecapacity1 mL vials1.5 mL vials350140 2104 mL vials100Microtiter platesDeep well platesInjection volume repeatabilityCarryoverInjection volume accuracyRepetition injection frequencyAnalytical run time settingInjection condition stepsOperating pressureRinsing liquid degassingMax.4 plates (up to 384 samples with 96 well or 1536 samples with 384 well plates)Max.4 plates (up to 384 samples with 96 plates)RSD < 0.3% (10μL injection)0.01% (under conditions determined by Shimadzu)±1% (50μL, n=6)1-99 / sample0.01-9999.9 min (0.01 min step)Max. 202 steps35.0MPa maximumStandardSamplecooler(LC-2010HT only)TypeTemperaturesetting rangeDirect cooling type.(For setting temperature at 4°C, ambienttemperature shall be 30°C or lower and humidity70% or lower.)Dehumidifier incorporated.4-40°C (For setting temperature at 4°C, ambienttemperature shall be 30°C or lower and humidity70% or lower.)Column OvenTypeTemperature setting rangeTemperature control precisionTemperature control rangeColumns accommodatedPreheater volumeBlock heating with preheating of the mobile phase4-60°C, 1°C step±0.1°C(Ambient temperature-15°C) -60°C2 x 250mm columns4μL (for Semi-micro), and 9μL, 2 channels16

UV DetectorControllerItem LC-2010HT (228-45103-3X) LC-2010HT (228-45102-3X)Light sourceDeuterium lamp,Low pressure mercury lamp (for wavelength accuracy check)Wavelength rangeBandwidthWavelength accuracyWavelength reproducibilityCellCell temperature controlDriftNoise levelLinearitySimultaneous dual wavelengthmeasurementSignal outputDisplayMemory mediaInput / output terminals190-600nm8nm±1nm±0.1nm10mm, 8μLStandard (Setting temperature: High, Low, and OFF, 3 steps)1 x 10 -4 AU / h (250nm, air in cell, constant, room temperature)*±0.25 x 10 -5 AU (2 sec. time, constant, 250nm, air in cell)*~2.5AUSelectable, two wavelengths in 190-370nm or 371-600nm2 channelsBacklit LCD display (320 x 240 dots)3.5 inch floppy disk drive (disk format, 2HD, MS-DOS)2 x external inputs, 2 x external outputs (+3 option),1 x optical link (for Chromatopac),1 x RS-232C (for connection to PC),2 x detector signal output (for integrator),BCD output of sample number (option)File numberMethod: 20 filesSystem preparation: 10 filesShut-down: 2 filesSequence: 1 fileSolvent delivery unit controlFlow rate, Concentration, Max. pressure, Min. pressure, Linear, Step, Exponential functionAuto sampler controlSample injection volume, Number of injections, Analysis time, Analysis file numberColumn oven controlDetector controlSelf diagnostics, Safety featureAutomation functionApplicable solventpH rangeLiquid contact surface materialsOperating Ambient temperature range, humidityPower requirementsSizeWeight*Condition according to ASTM E1657-96Oven temperature, Max. temperatureWavelength, Time constant, Lamp ON / OFF, Integrator output rangeMemory check, Max / Min pressure, Oven max. temperature, Lamp current, motor error check, Leaksensor, Lamp cover sensor, Lamp housing max. temperatureAuto start-up, Auto shut-down, Baseline stability autojudgment,Auto validation (Wavelength, Lamp energy, Solvent delivery pulsation, Column temperature, Absorbance,Baseline drift, Baseline noise, Pressure limit, Gradient accuracy)Auto purge, Cell auto recognition, Column management (option)Memory check, Max / Min pressure, Oven max. temperature, Lamp current, Motor revolution monitor,Leak sensor, Lamp cover sensor, Lamp housing max. temperatureOrganic solvents (except fluorine solvent, e.g. HFIPA),Aqueous solution and mixture of diluted acid or base(except halo-acids, e.g. hydrochloric acid)1-13 standardSUS316, Ruby, Sapphire, PTFE, Hasteloy C, PEEK, Trifluoroethylene, PFA Perfluoroelastomer, Quartz,Ceramic4-35°CMax. 80% humidityAC 100V-240V, 700VA, 50 / 60HzW430 x D500 x H605 mm52kg47kg17

OptionsOptionsOptions for LC-2010HTDescription Part Number Set NoSample rack for 1mL vials (2 racks / set)Sample rack for 1.5mL vials (2 racks / set)*228-37532-91228-45409-91228-44628-91For LC-2010AHT / 2010CHT (up to 350 vials)For LC-2010AHT (up to 210 vials)For LC-2010CHT (up to 140 vials)Sample rack for 4mL vials (2 racks / set)Sample rack for Microtiter plate (2 racks / set)Sample rack for Deep-well plate (2 racks / set)Column Management Device (CMD)CMD cableOptional board228-37544-91228-37545-91228-37546-91228-37281-91228-39991228-40050-91For LC-2010AHT / 2010CHT (up to 100 vials)For LC-2010AHT / 2010CHT (up to 4 vials)For LC-2010AHT / 2010CHT (up to 4 vials)For column management (1 device for 1 column)1 cable is required for 1 LC-2010AHT / 2010CHTTo add functions of:1) BCD output of sample number2) addition of event output terminal3) analog output of pump pressure, column oven temperature,ambient temperature sensor, sample cooler temperatureRecycle valveTemperature-controlled flow cell for semi-micro HPLC2mL sample loop kitMaintenance kit without toolsMaintenance kit with tools* Sample rack for 1.5mL vials (2racks / set) is included as standard accessory.228-37383-91228-37384-91228-37548-91228-39872-96228-39872-97For solvent recyclePass length: 5mm, cell volume: 24μLFor large-volume injectionVials for AutosamplerRacksApplicable Sample VialsSample Rack for 1mL Vials11mL Glass (with PE cap)250pcs / set(228-39699-91)21.5mL Glass (with cap,silicone rubber septum)100pcs / set(228-15652-92)31mL PP*(with PE cap)200pcs /set(228-31600-91)40.2mL PP*(with PE cap)100pcs / set(228-35217-91)Sample Rack for 1.5mL Vials51.1mL Glass*(with cap, silicone rubberseptum)100pcs / set(228-21283-91)60.3mL Glass*(with metal spacer, cap, silicone rubber septum)100pcs / set (228-16847-92)(Vials without spacer as spare)100pcs / set (228-16850-91)Sample Rack for 4mL Vials74mL Glass(with cap, silicone rubberseptum)100pcs / set(228-21287-91)84mL PP*(with PE cap)100pcs / set(228-31537-91)90.3mL Glass*(with spring to be inserted in 4mL vial)100pcs / set (228-21284-91)(Vials without spring as spare)100pcs / set (228-21285-91)• All vials commonly used with the SIL-20A / 10ADVP / 10AF / 10Ai / HT can be used with the LC-2010HT.• Regarding vials, microtiter plates, and deep-well plates marked*, the set temperature and actual temperature might differ due to differences in shape or in the thermalconductivity of the materials.• Silicon rubber septa (with PTFE cover) (228-26718-93,100pcs) or PTFE septa (228-15655-91,100pcs) can be chosen for vials No. 2, 5, and 6.• Silicon rubber septa (with PTFE cover) (228-21290-91,100pcs) or PTFE septa (228-23469-91,100pcs) can be chosen for vials No. 7, 8, and 9.• Please use commercially available microtiter plates and deep-well plates.• PP: polypropylene, PE: polyethylene18

Technical BulletinEnhancing the Accurancy of Low-pressure GE systemsHPLC gradient (GE) systems are classified as high-pressure GEsystems or low-pressure GE systems, according to the unitconfiguration. This bulletin describes how to make stableconcentration settings with a low-pressure GE system, based onthe LC-2010HT.Solenoid Valve(Mobile Phase Mixing Valve) DriveTo reduce the dead volume and flow pulsations,the LC-2010HT uses a serial double-plunger pump, incorporating aprimary pump head with a 10μL plunger capacity. A low-pressure GEsystem mixes multiple mobile phases by switching the pump solenoidvalve in synchronization with the pump intake stroke. A low-capacityplunger pump that requires frequent solenoid valve switching isunfavorable for accurate concentration settings.However, the LC-2010HT eliminates this problem by allocating 100%concentration over four pump intake cycles, as shown inFig.1.Fig.1 shows the flow rate changes in the pump head and the solenoidvalve operation timing for a concentration setting of B: 37.5%,A:62.5%.Correction for Effects due to Mobile PhaseCompressibilityFig. 2 is an enlarged view of the transition from discharge tointake for the pump shown in Fig. 1. The point (1) in the diagramis where the pump plunger starts to retract from its fully advancedposition. At this point, the pump head interior still retains highpressure from the discharge stroke. The mobile phase starts toflow into the pump head after the plunger retracts and allows thecompressed mobile phase to expand back to atmosphericpressure inside the pump head ((2) in Fig. 2).The hatched area in Fig. 2 represents the area where no mobile phaseintake occurs. As this area increases in size and concentration errorsincrease at higher pressures, the solenoid valve operation timing isadjusted according to the monitored pressure values to reduce theconcentration errors. Solenoid valve operation can be matched to themobile phase compressibility by entering the compressibility value.Correction for Errors due to Dalay inSolenoid Valve ResponseA millisecond-order delay occurs before the solenoid valve operatesafter the signal is input. Consequently, the solenoid valve is operatedearly by a time corresponding to this delay.Correction of Mechanical ErrorsMechanical errors in cams and other parts of the plunger drive trainaffect the concentration accuracy. The LC-2010HT is accuratelycalibrated before dispatch from the factory for the difference betweenthe set concentration and measured values.Using the techniques above, the LC-2010HT achieves a concentrationaccuracy of ±0.5% (water/acetone mixture at 0.1 to 2.0mL / min. and 1to 20MPa).1 2Fig. 2Flow Rate in Pump HeadTimeDischargeB B A A A BIntakeValve B open (B: 37.5%)Valve B openValve A openValve A open (A: 62.5%)Fig. 119

Combination of the LC-2010HT and the LCMS-2010EVrealizes an even slimmer LC-MS systemSample Carryover Reduced toExtreme LimitsFor LC/MS analysis, where sensitivity is a keyfactor, it is essential to have an autosamplerdesigned to minimize sample carryover.The LC-2010HT system combined with theSIL-HT autosampler reduces sample carryover toextreme limits.(x1,000,000)(x1,000)43cm 45cmThe LCMS-2010EV with an LC-2010HT system:Space requirements, analysis, and initial costshave all been slimmed down.2.52.01.51.00.545113877m/z 505.1Highly ConcentratedChlorhexidine 10μL injected(Area:45113877)782m/z 505.10.00.0 5.0 min. 0.0 5.0 min.5.04.54.03.5Blank (Mobile Phase)10μL injected (Area:782)0.0017% Sample CarryoverJQA-0376Founded in 1875, Shimadzu Corporation, a leader in thedevelopment of advanced technologies, has a distinguishedhistory of innovation built on the foundation of contributing tosociety through science and technology. We maintain a globalnetwork of sales, service, technical support and applicationscenters on six continents, and have established long-termrelationships with a host of highly trained distributors locatedin over 100 countries. For information about Shimadzu, and tocontact your local office, please visit our Web site atwww.shimadzu.comSHIMADZU CORPORATION. International Marketing Division3. Kanda-Nishikicho 1-chome, Chiyoda-ku, Tokyo 101-8448, JapanPhone: 81(3)3219-5641 Fax. 81(3)3219-5710URL http://www.shimadzu.comThe contents of this brochure are subject to change without notice.Printed in Japan 3295-01705-30A-NS