8.000.5051EN - Metrohm

Wine analysisQuality assurance of wine and wine-based drinks

02Metrohm ...• is the global market leader in titration• is the only company to offer a complete range of ion analysis equipment – titration,voltammetry, and ion chromatography• is a Swiss company and manufactures exclusively in Switzerland• grants a 3-year instrument warranty and a 10-year warranty on chemical suppressors forion chromatography• provides you with unparalleled application expertise• offers you more than 1300 applications free of charge• supports you with expert service through local representatives and regional support centers• is not listed on the stock exchange, but is owned by a foundation• gives the interests of customers and employees priority over maximizing profit

Metrohm – customized analysis for the wine industryThe law sets high standardsThe wine industry is subject to strict regulations on thequality and safety of its products. This is not without reason.If contaminated foodstuffs reach the retail market,the consequences can be dire – not just for consumers.Regulatory complianceIn order to guarantee the industry’s strict quality andsafety standards, reliable instruments and methods areneeded in the QC laboratory. These instruments andmethods must meet highest standards. «Regulatory compliance»is the catchword here.Count on our supportAs a leading manufacturer of instruments for chemicalanalysis, we are quite aware of these challenges you face.For this reason, we offer you not just the most advancedequipment but rather complete solutions for the particulartask at hand. Your partners at Metrohm are experiencedprofessionals, who develop customized app li cationsfor you and support you in every aspect of regulatorycompliance with expert service.On the following pages, discover the solutions Metrohmoffers the wine industry to safeguard the quality of theirproducts.03Metrohm instruments comply with major official standards,including the FDA regulation Title 21 CFR Part 11

Methods available from Metrohm for the wine sector04Metrohm is your partner for all matters concerningche mi cal wine analysis and the validation of analyticalmethods. Apart from complying with official regulatoryre quirements, Metrohm instruments and app licationssatisfy all the standard test methods for quality controland product release.The following table is organized according to productareas and lists relevant parameters that can be determinedusing methods developed by Metrohm. If you can notfind a parameter that is important for you, please contactus. We are continuously expanding our range of applicationsand will gladly assist you in meeting your specificanalytical requirement.MethodpH measurementTitrationIon chromatographyVoltammetryParameterspH valueTotal titratable acidFree sulfurous acidTotal sulfurous acidAscorbic acidVolatile acidsCO 2contentAsh alkalinityChlorideSulfateAcids such as peracetic acid,pH value and conductivity(in the rinsing, cleaning anddisinfection process)Decarbonation(Automated sample preparation)Anions: chloride, nitrate,phosphate, malate, tartrate,sulfite and sulfateCations: sodium, potassium,magnesium, calcium,manganese and histamine withputrescine and cadaverineorganic acids and phosphateMono-, di-, and oligosaccharidesSugar alcoholsGlycolsHeavy metals: cadmium, lead,copper, iron, mercuryLiteratureApplication Bulletin 225 −Simple wine analysisApplication Bulletin 225 –Simple wine analysisWine PACMonograph: Practical TitrationApplication Note S 281Monograph: Practical ICApplication Note C 070Application Note O 006Application Note V 093Page56911

pH measurementThe pH value is of paramount importance for biologicalsystems. It influences the growth of microorganisms, thecolor and hue, the taste, the redox potential, the ratio offree to bound SO 2, the possibility for forming or preventingiron phosphate cloudiness, etc.05There is no direct correlation between the pH value andthe content of total titratable acid, but there is an (em -piri cal) relationship between the pH value and the ratioof potassium hydrogen tartrate to tartaric acid.For wines and spirits, the flat membrane electrode is re -commended as a universal pH electrode. The Unitrodeexcels in its short ad justment times following temperaturechanges and is protected from contamination by itsfixed ground-joint diaphragm.pH value in wine samples with the pH meter• Easy to use thanks to a well-structured multiple-linedisplay• Automatic buffer recognition• Robust electrodes• Extensive range of accessories from a single sourceVery cost-effective pH meter − the 827 pH lab as a laboratory setupBuffer sachetsBuffer solutions pH 4, 7, and 9 in sachets guarantee precisecalibration of the pH electrode826 pH mobile as a portable pH meter for on-site use

06TitrationBenefit from the know-how of the market leaderChoosing the right electrode is crucial for successful titration.Metrohm is the leading manufacturer in the field oftitration. More than 60 years of experience and the mostcomprehensive and innovative portfolio on the marketare proof of our strength. Tap into our extensive collectionof potentiometric applications and benefit from theexpertise of our application laboratories.Of course, our applications cover the different regional/national determination methods and guarantee compliancewith the respective official standards.Besides choosing the right kind of diaphragm, the responsebehavior of the electrode is also important, inparticular, if the titration is to be carried out to a definedendpoint. Metrohm offers an appropriate electrode forevery need. Further practical tips on using Metrohm electrodesare given in the leaflets «Electrodes for titration»and «Elec trodes for pH Measurement». These can bedown loaded for free at www.metrohm.comArea of application Metrohm electrode FeaturespH value Flat membrane • Ideal for determining total acid contentAcid content of alcoholic drinks electrodeand measuring the pH value in wine• Designed for small sample volumesSulfite in wine Double Pt electrode • Robust• Long working life• Easy to cleanStabilization through sulfiteIn some parts of the wine industry, there are efforts toproduce wines without the addition of sulfur dioxide. Forthe past few years, a small number of conventional producersand also organic producers have managed to dothis successfully, thanks primarily to cellar technology.Pro ducing naturally sweet wines without sulfur is, however,impossible, because the high residual sugar contentsprovide an ideal nutrient medium for microorganisms ofall kinds. Depending on the particular region or kind ofwine, different maximum limits for residual SO 2apply.

Titration with Metrohm: Solutions for every needand budgetWith the 848 Food/Beverage Titrino plus and the 905Food Titrando, we offer our customers two completetitration packages. No matter which package you choose,simple and reliable work is guaranteed:07• Intelligent exchange or dosing units ensure the use ofthe correct titration solution and permit their monitoring• All important titration parameters are stored with therespective methods; this means that even unskilledusers quickly obtain reliable results• GLP-compliant data output either on a printer or bydigital archiving814 USB Sample ProcessorDepending on the application, different sample racks canbe used on the 814 USB Sample Processor, e.g., 22 samplebeakers of 120 mL.Both the measuring of the prescribed sample quantityand the complete decarbonation of the wine samples areperformed fully automatically. The costs of automationpay for themselves within a very short time and reproducibilityis also improved.Control of the system and all the data handling are takencare of by the tiamo TM software.tiamo TM is the leading control and database software fortitrators and dosing devices as well as for complete laboratoryautomation. This is why the name tiamo TM standsfor «titration and more» – tiamo TM can do much morethan just tit rate. tiamo TM is a titration network (NTDS =Networked Titra tion Data System).815 Robotic USB Sample Processor XLAutomated system for the decarbonation and titration oftotal acid and sulfurous acid. Up to 100 samples can beplaced on the rack. Following titration, the external vesselis cleaned thoroughly by the integral membrane pump.

08Laboratory automation – and everything goes somuch faster!Automation = time savings and higher accuracyWith instruments from the Metrohm Robotic familyneither erroneous o results nor time-consuming manualroutines are an issue. Thanks to modern automation,precise results are obtained irrespective of who the useris. Sea sonal overload in the laboratory will belong to thepast.In particular, the decarbonation of carbonated drinks isstill frequently performed manually. Consequently, considerablymore time is spent on preparing the sample byboiling it or treating it with ultrasound than on the analysisproper.Fast decarbonationAs a specialist in laboratory automation we, offer you acustomized, fully automated system for decarbonation.Simply place the samples in the sample rack – all the restis done fully automatically. Following very quick and effectivedecarbonation, the samples are measured automaticallyand transferred into an external titration vessel.Automation from the market leader means:You save time• Precise measurement of samples is no longer doneby you, but by the system − of course, that appliesto the determination of total acid content as wellas to sulfite determination, etc.• Fully automated decarbonation.You save money• Every day. The time saved leads to a noticeableimproved profitability right from day one.• Your investment soon pays for itself.• Fewer errors in sample handling and titration

Ion chromatographyIon chromatography (IC) is a standard method for wineanalysis. Numerous major and minor compounds thataffect taste and nutrition as well as traces of conta minantscan be reliably and precisely determined by IC.Multicomponent determinations in a single analysisIn addition to anions and cations, sugar compounds, or -ga nic acids and polar substances can also be quantifiedin a variety of wines. The advantage of ion chromatographyis that chemically similar substances can be determinedsimultaneously in a single analysis. Of course, allMetrohm IC instruments and the MagIC Net TM chromatographysoftware comply with FDA regulations.Application exampleAnalysis of acids in wineAcids are an important component in wine. They preservethe wine and give it expression and character. Winecontains several kinds of acids. The most important onesare tartaric acid, malic acid, and lactic acids, as well ascar bo nic acid in sparkling wines.Analysis of biogenic amines in wineApart from the various acids, it is also possible to detectthe biogenic amines produced during malolactic fermentation,e.g., histamine, tryramine, putrescine, and phenylethylamine,with ion chromatography.09181614Conductivity [µS/cm]12108642chloridesuccinatemalatesulfitetartratesulfate00 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18Time [min]19 20Anion and organic acid analysis in wine: column: Metrosep A Supp 10 - 100/4.0;eluent: 5.0 mmol/L Na 2CO 3+ 5.0 mmol/L NaHCO 3, 1.0 mL/min; sample volume: 20 μL,inline dilution: 1:10Inline Eluent PreparationThe time-consuming manual preparation of eluents is athing of the past. Automatic inline preparation of eluentscan be easily done by expanding the IC system with anadditional Dosino and a Level Control. While the LevelControl mo ni tors the eluent level, the Dosino performsall dosing and liquid handling tasks.

The most efficient form of analysis10Flexibility creates numerous opportunitiesIon chromatography is hard to beat on wine analysis.Anions, cations, organic acids, amines, carbohydrates –all can be detected with a single system. Automation ofthe IC system reduces manual steps to a minimum andin creases reproducibility and accuracy.MagIC Net TMMagIC Net TM is the powerful ion chromatography sofwarethat guarantees efficient working.The advantages of MagIC Net TM• Easy and intuitive operation• Freely configurable user interface• Great flexibility• Modern data management• Powerful report generator• Comprehensive monitoring and control functions• Complete traceability of all data• Client-server software• Complies with all FDA and GLP requirements• Available in many different languagesAnalysis of awine standard for Li + , Na + , NH + 4, K + , Mg 2+ , Ca 2+ , putrescine, cadaverine, andhistamine by means of 850 Professional IC; column: Metrosep C 1 - 125/4.6; eluent: 2.5 mmol/LHNO 3with 10% acetone; sample volume: 10 μL, Inline Dilution: 1:10What automation from the technology leader means for you:You save time due to• Metrohm Inline Dilution• Metrohm Inline Eluent PreparationYou save money thanks to• low operating costs• a 10-year suppressor warranty• a 3-year warranty on all instruments• guaranteed availability of spare parts 10 years afterinstrument discontinuation date

11VoltammetrySuperior detection sensitivity, competitive priceVoltammetry is an electrochemical analytical method thatprovides information on the type and amount of substancescontained in a dissolved sample on the basis ofa current-voltage relationship. The particular benefit ofvol tammetry lies in its high accuracy and sensitivity, thepos sibility of performing speciation analysis, and its fa vorableprice-performance ratio.Heavy metal ions that are present as contaminants infood can be determined with high sensitivity by voltammetry.To this end, food samples must be digested priorto analysis.797 VA ComputraceThe 797 VA Computrace is a state-of-the-art, computerassistedvoltammetric analysis system. The potentiostat/galvanostat built into the instrument guarantees highestprecision with reduced noise. The included PC softwarecontrols the determination and calculates and archivesthe results.Voltammetric determination of Cd, Pb and Cu in wineApplicationsCd, Pb, Cu, Fe(II), Fe(III) and HgMost heavy metals are toxic and very low maximum limitsapply regarding their presence in wine. Thus the exactdetermination of these elements by high-performancemethods is essential. With the 797 VA Computrace, it ispossible to perform the determination of heavy metals inwine and other foods after UV digestion.

Service that you can rely on:the Metrohm Quality Service12Reliable results for the lifetime of the analyticalinstrumentMetrohm analytical instruments are engineered to deliverextremely precise measurements. As a result, leading in -ter national companies from the food industry also puttheir trust in us for our comprehensive services. Theseservices ensure that laboratory managers can rely 100%on results produced during the entire lifetime of theirMetrohm analytical instruments.Regulatory compliance made easyThe food industry is required to comply with all effectivelaws and guidelines on food safety. Through MetrohmCompliance Service, we ensure that your company complieswith standards such as IFS, BRC, SQF and ISO 22000.If you want to put a Metrohm analytical instrument intooperation, we take care of everything else.This includes:• Competent assistance with Design Qualification (DQ)• Professional installation through compliantInstallation Qualification (IQ)• Operational Qualification (OQ) guaranteeing thatMetrohm instruments meet the equipmentspecification• All qualification and validation work, which is performedonly by professionally trained and certifiedMetrohm service specialists• User training taught by experts• Competent requalification and revalidation

Why Metrohm Quality Service?The Metrohm Quality Service is available worldwide.Pre ven tive maintenance carried out on a regular basisex tends your instrument’s lifetime while providing fortrouble-free operation. All maintenance work done un -der the label Metrohm Quality Service is carried out byour own certified service experts. You can choose bet -ween different types of service contracts. With a fullservice contract, for example, you can rely on the optimumperformance of your Metrohm instruments at anytime, incur no additional costs whatsoever and benefitfrom complete and compliant verification documentation.Thanks to our service, you are perfectly prepared foraudits.13An overview of Metrohm Quality ServiceOur ServicesApplication support by means of our vast selectionof Application Bulletins, Applications Notes,monographs, validation brochures, technical posters,and articlesPersonal consultation by our specialists per telephoneor e-mailTraining coursesCertified calibrations, for example of dosing andexchange unitsRemote maintenanceBack-up supportEmergency service, for example express on-site repairsOriginal spare parts, made in Switzerland and availableworld wideGuaranteed spare parts available for at least 10 yearsbeyond instrument discontinuation dateDecentralized repair workshops located around theworld and a central workshop in SwitzerlandCustomer benefitQuick and professional solution to all arising applicationquestions and complex analytical challengesCompetent users contribute substantially toreliable resultsAccurate measurementsVerification documentation for compliance withregulations and for efficient auditsExpeditious resolution of software questionsHigh data securityShort response times and thus, rapid problem resolutionMinimization of downtimeLasting, successful repair; short delivery timesMinimization of downtimeProtection of your investment through long-termavailability of spare parts and accessoriesQuality repairs done quickly, so your instrumentsare ready for use againThanks to Metrohm Quality Service, you can rely on your results for the lifetime of the analyticalinstrument.

Technical specifications and ordering information14pH value2.827.0214 827 pH Lab Irda 230 V EU with Unitrode2.827.0215 827 pH Lab Irda 120 V US with Unitrode2.827.0217 827 pH Lab Irda 240 V AUS with Unitrode2.827.0219 827 pH Lab Irda 230 V UK with UnitrodeTotal titratable acids (other potentiometric titrations require optional accessories)2.848.1010 Food & Beverage Titrino plus2.848.1020 Food & Beverage Titrino plus with printer2.905.4010 Food Titrando with tiamo TM light2.862.1010 Food & Beverage Compact TitrosamplerOptional accessories for the determination of free or total sulfurous acid sulfite6.0309.100 Double Pt Foil Electrode6.2104.020 Electrode cableFor instruments and accessories for other applications and their automation please contactyour local Metrohm distributor.Ion Chromatography2.850.3030 850 Professional AnCat – MCS for anion and cation analysis2.881.0030 881 Compact IC pro – Anion – MCS2.858.0020 858 Professional Sample Processor for automation2.800.0010 800 Dosino for Metrohm Inline Dilution2.849.1030 849 Inline Eluent Preparation6.2841.100 Rinsing Station for IC Sample Processor6.3032.210 Dosing Unit 10 mL6.5330.020 IC equipment for Metrohm Inline Dilution6.5330.040 IC equipment for liquid handling with Dosino6.1020.010 Metrosep A Supp 10 - 100/4.0 for anion and sulfite analysis6.1020.500 Metrosep A Supp 10 Guard/4.06.1010.100 Metrosep C 1 - 125/4.6 for standard cation and biogenic amine analysis6.1050.420 Metrosep C 4 - 150/4.0 for standard cation and transition metal analysis6.1050.500 Metrosep C 4 Guard/4.06.6059.222 MagIC Net TM 2.2 Professional

15Voltammetric metal determination2.797.0010 797 VA Computrace for manual operationMVA-2VA Computrace system with automatic standard addition. Consisting of 797 VA Computracewith two 800 Dosinos.MVA-3Fully automated VA Computrace system consisting of 797 VA Computrace with863 Compact VA Autosampler and two 800 Dosinos for automatic addition of auxiliarysolutions. Enables automatic processing of up to 18 samples. This system is the optimumsolution for automatic analysis of small series of samples.Sample preparation for voltammetry2.705.0016 705 UV Digester (220 V / 60 Hz) for the fast and efficient digestion of wine samples for thedetermination of transition metals.2.705.0017 705 UV Digester (220 V / 50 Hz) for the fast and efficient digestion of wine samples for thedetermination of transition metals.

Subject to change without prior noticeLayout by Ecknauer+Schoch ASW; printed by Metrohm AG, 9101 Herisau, Switzerland8.000.5051EN – 2011-03wine.metrohm.com