Amino Acid Analyzer - Metrohm
Amino Acid Analyzer - Metrohm
Amino Acid Analyzer - Metrohm
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<strong>Amino</strong> <strong>Acid</strong> <strong>Analyzer</strong><br />
Automated analysis of amino acids
<strong>Amino</strong> acid analysis made easy<br />
The <strong>Amino</strong> <strong>Acid</strong> <strong>Analyzer</strong> is an automated system for the<br />
highly accurate determination of amino acids. Reliable<br />
routine measurements and fl exible tests for method<br />
development can both be performed on this equipment.<br />
Highlights<br />
• Robust analysis of up to 44 amino acids in a wide variety of sample matrices<br />
• Standardized methods based on VIS-detection after derivatization with ninhydrin in<br />
accordance with USP <br />
• Completely automated analyzer with cooling option for up to 384 samples<br />
• Entirely metal-free system for the analysis of biologically active samples<br />
• High detection sensitivity<br />
• Also suitable for analyzing anions and cations
Robust and precise<br />
Do you want to analyze amino acids Do you have to<br />
comply with the test methods listed in the pharmacopoeias,<br />
e.g. USP (Method 1) By combining the<br />
886 Professional Thermostat / Reactor with the 887 Professional<br />
UV/VIS Detector you can work precisely and<br />
accurately in compliance with the standards.<br />
650<br />
600<br />
550<br />
500<br />
450<br />
Gly<br />
Intensity [mAU]<br />
400<br />
350<br />
300<br />
250<br />
200<br />
150<br />
Asp<br />
Ser<br />
Thr<br />
Glu<br />
Ala<br />
Val<br />
(Cys)2 Met<br />
Ile Leu<br />
Tyr<br />
Phe<br />
NH 3<br />
Lys<br />
His<br />
Arg<br />
100<br />
50<br />
Pro<br />
0<br />
0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80<br />
Time [min]<br />
85 90<br />
<strong>Amino</strong> acids (0.5 mM; 20 components). Column: Metrosep <strong>Amino</strong> <strong>Acid</strong>s 1 - 100/4.0, eluent:<br />
lithium citrate gradient similar to USP (Method 1), 0.4 mL/min, 60 °C, postcolumn<br />
reaction: ninhydrin reagent, 0.2 mL/min, 120 °C, wavelength 570 nm.<br />
For determination of amino acids in compliance with USP (Method 1), the 886 Professional<br />
Thermostat / Reactor ensures the required 120 °C for postcolumn derivatization.
Ordering information<br />
2.887.0010 887 Professional UV/VIS Detector<br />
2.886.0110 886 Professional Reactor<br />
2.872.0010 872 Extension Module IC Pump<br />
2.850.1220 Professional IC Cation – HP Gradient<br />
2.889.0020 889 IC Sample Center – cool<br />
6.4001.410 Metrosep <strong>Amino</strong> <strong>Acid</strong>s 1 - 100/4.0<br />
www.metrohm.com<br />
Subject to change<br />
Layout by Ecknauer+Schoch ASW, printed by <strong>Metrohm</strong> AG, CH-9101 Herisau<br />
8.887.5002EN – 2010-06
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