Amino Acid Analyzer - Metrohm

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Amino acid analysis made easy The Amino Acid Analyzer is an automated system for the highly accurate determination of amino acids. Reliable routine measurements and fl exible tests for method development can both be performed on this equipment. Highlights • Robust analysis of up to 44 amino acids in a wide variety of sample matrices • Standardized methods based on VIS-detection after derivatization with ninhydrin in accordance with USP • Completely automated analyzer with cooling option for up to 384 samples • Entirely metal-free system for the analysis of biologically active samples • High detection sensitivity • Also suitable for analyzing anions and cations
Robust and precise Do you want to analyze amino acids Do you have to comply with the test methods listed in the pharmacopoeias, e.g. USP (Method 1) By combining the 886 Professional Thermostat / Reactor with the 887 Professional UV/VIS Detector you can work precisely and accurately in compliance with the standards. 650 600 550 500 450 Gly Intensity [mAU] 400 350 300 250 200 150 Asp Ser Thr Glu Ala Val (Cys)2 Met Ile Leu Tyr Phe NH 3 Lys His Arg 100 50 Pro 0 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 Time [min] 85 90 Amino acids (0.5 mM; 20 components). Column: Metrosep Amino Acids 1 - 100/4.0, eluent: lithium citrate gradient similar to USP (Method 1), 0.4 mL/min, 60 °C, postcolumn reaction: ninhydrin reagent, 0.2 mL/min, 120 °C, wavelength 570 nm. For determination of amino acids in compliance with USP (Method 1), the 886 Professional Thermostat / Reactor ensures the required 120 °C for postcolumn derivatization.
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Amino Acid Analyzer - Metrohm

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