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Amino Acid Analyzer - Metrohm

Amino Acid Analyzer - Metrohm

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Robust and precise<br />

Do you want to analyze amino acids Do you have to<br />

comply with the test methods listed in the pharmacopoeias,<br />

e.g. USP (Method 1) By combining the<br />

886 Professional Thermostat / Reactor with the 887 Professional<br />

UV/VIS Detector you can work precisely and<br />

accurately in compliance with the standards.<br />

650<br />

600<br />

550<br />

500<br />

450<br />

Gly<br />

Intensity [mAU]<br />

400<br />

350<br />

300<br />

250<br />

200<br />

150<br />

Asp<br />

Ser<br />

Thr<br />

Glu<br />

Ala<br />

Val<br />

(Cys)2 Met<br />

Ile Leu<br />

Tyr<br />

Phe<br />

NH 3<br />

Lys<br />

His<br />

Arg<br />

100<br />

50<br />

Pro<br />

0<br />

0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80<br />

Time [min]<br />

85 90<br />

<strong>Amino</strong> acids (0.5 mM; 20 components). Column: Metrosep <strong>Amino</strong> <strong>Acid</strong>s 1 - 100/4.0, eluent:<br />

lithium citrate gradient similar to USP (Method 1), 0.4 mL/min, 60 °C, postcolumn<br />

reaction: ninhydrin reagent, 0.2 mL/min, 120 °C, wavelength 570 nm.<br />

For determination of amino acids in compliance with USP (Method 1), the 886 Professional<br />

Thermostat / Reactor ensures the required 120 °C for postcolumn derivatization.

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