Amino Acid Analyzer - Metrohm
Amino Acid Analyzer - Metrohm
Amino Acid Analyzer - Metrohm
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Robust and precise<br />
Do you want to analyze amino acids Do you have to<br />
comply with the test methods listed in the pharmacopoeias,<br />
e.g. USP (Method 1) By combining the<br />
886 Professional Thermostat / Reactor with the 887 Professional<br />
UV/VIS Detector you can work precisely and<br />
accurately in compliance with the standards.<br />
650<br />
600<br />
550<br />
500<br />
450<br />
Gly<br />
Intensity [mAU]<br />
400<br />
350<br />
300<br />
250<br />
200<br />
150<br />
Asp<br />
Ser<br />
Thr<br />
Glu<br />
Ala<br />
Val<br />
(Cys)2 Met<br />
Ile Leu<br />
Tyr<br />
Phe<br />
NH 3<br />
Lys<br />
His<br />
Arg<br />
100<br />
50<br />
Pro<br />
0<br />
0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80<br />
Time [min]<br />
85 90<br />
<strong>Amino</strong> acids (0.5 mM; 20 components). Column: Metrosep <strong>Amino</strong> <strong>Acid</strong>s 1 - 100/4.0, eluent:<br />
lithium citrate gradient similar to USP (Method 1), 0.4 mL/min, 60 °C, postcolumn<br />
reaction: ninhydrin reagent, 0.2 mL/min, 120 °C, wavelength 570 nm.<br />
For determination of amino acids in compliance with USP (Method 1), the 886 Professional<br />
Thermostat / Reactor ensures the required 120 °C for postcolumn derivatization.