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2644 - CSUN ScholarWorks - California State University, Northridge

2644 - CSUN ScholarWorks - California State University, Northridge

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Journal of Student Research Abstracts · 39<br />

within the dead tobacco infectious. A similar control solution was made without the buffer. After 3 weeks of<br />

growth, the plants were infected with TMV with a sterile brushing of the inoculum on the leaves. TMV causes<br />

quantifiable lesions in pinto (Phaseolus vulgaris). Lesion counts were daily taken, and while significant differences<br />

appeared initially, after complete virus propagation, no significant differences existed in the number<br />

of lesions per square centimeter. Zinc seems to slow viral attack, but at this concentration does not stop the<br />

virus. Subsequently, to see if more slowing would occur with more zinc, a similar trial was done with varying<br />

zinc nitrate concentrations. With an identical technique, 3 groups were grown with either distilled water, 5 ppm<br />

zinc nitrate or 50 ppm zinc nitrate. After infection, the higher zinc concentration showed much slower symptom<br />

emergence than the water and 5 ppm zinc groups. But in the end results showed no significant difference.<br />

Thus, according to this data, zinc's presence slows the propagation ofTMV in pinto plants.<br />

2722<br />

THE EFFECT OF VITAMIN K ON THE LEARNING RATE OF PLANARIA.<br />

Michelle Thomas, and Steve DeGusta (teacher). John F. Kennedy High School, 6715 Gloria Drive,<br />

Sacramento, CA 95831.<br />

This experiment was done to determine if vitamin K made planaria learn faster. This experiment compared<br />

the learning rate of planaria exposed to 0.0012% vitamin K solute with a control group of normal planaria. The<br />

planarian training consisted of exposure to a brief pulse of light followed by an electric shock. This process<br />

was repeated 25 times daily until the planaria reacted to the light as it would to shock 75% of the time. After<br />

the planarian responded correctly 75% in one test consisting of 25 light shock repetitions it was said to have<br />

learned the condition. No significant differences were found in the learning rates of the two groups of planaria<br />

(0.9>P>0.5). This investigation found that vitamin K did not increase the learning rate in which planaria learn.<br />

2723<br />

NUTRITION STUDIES WITH RED WORMS.<br />

Ryan Sunahara, Steve DeGusta (teacher). John F. Kennedy High School, 6715 Gloria Drive, CA 95831.<br />

In my lab, I tested Red worms and their diets to see if it affected their growth rate. I gathered forty worms<br />

and placed them in forty different petri dishes. Each dish contained peat moss for their bedding and their<br />

assigned diet. There are three different diets: fish food, com starch, and fish food plus com starch. I also had<br />

one petri dish with only peat moss for my control. Before placing the worms in the dish, I measured their initial<br />

lengths by carefully laying them against a ruler. After ten days of living in their petri dish environment, I<br />

measured the new lengths of the worms. After another seven days, I measured the worms once again. I found<br />

that the fish food plus com starch group had the greatest growth rate among the four groups. The fish food<br />

group, com starch, and control group were in descending order of growth rates respectively. The results that I<br />

received were as expected. The fish food plus corn starch diet was the most balanced of the three other diets<br />

and, therefore, it had the largest growth rates among the worms. A few worms in each group did die and were<br />

disregarded when doing the statistics. All the worms that were alive at the end of the two and half week period<br />

had grown between 0.4 em and 1.1 em. Using statistics, I found that my measurements in lengths of the<br />

worms had no significant difference between the groups. Although all the worms did grow, the difference<br />

between the groups were found to be insignificant.

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