2644 - CSUN ScholarWorks - California State University, Northridge

2736
Journal of Student Research Abstracts · 45
THE ACTIONS OF RENU MULTIPLUS MULTI-PURPOSE SOLUTION ON
B. CEREUS
Bryan James Fang and Steve DeGusta (teacher). John F. Kennedy High School, 6715 Gloria Drive,
Sacramento, CA 95831.
In this investigation, I tried to show if contact lens solution (Renu Multiplus Multi-purpose solution)
inhibits the growth of bacteria (B. cereus). The Renu Multiplus Multi-purpose solution contains the ingredient
Dymed that is supposed to kill microorganisms on contact lenses. B. cereus is grown on agar containing contact
lens solution. I used the pour-plating method to transfer the bacteria to the agar. I wanted no bacteria grown
on the agar when it is incubating. I used twenty-one petri dishes. I used different concentrations for these petri
dishes. I labeled these concentrations as agar #1, 2, 3, 4. Each agar contained different concentrations.# 1 agar
contained 50 ml of water for 1.15 g of nutrient agar. #2 agar contained 5 ml of contact lens solution and 45 rnl
of water for 1.15g of nutrient agar. #3 agar contained 25 rnl of contact lens solution and 25 ml of water for
1.15g of nutrient agar. #4 agar contained 50 ml of contact lens solution for 1.15g of nutrient agar. Five petri
dishes were used for each group of agar. In addition, a single petri dish contained B. cereus grown on agar with
paper filter disks containing contact lens solution. This is called the additional experiment. After this the petri
dishes were incubated at room temperature. My results showed that the contact lens solution is effective in
inhibiting the growth of bacteria. Agar #2, 3, 4 inhibited the growth of bacteria. The paper filter disks containing
contact lens solution in the additional experiment, also showed effectiveness. Around each paper filter
disk (except water) were zones of inhibition.
2737
THE EFFECT OF NEOSPORIN OINTMENT ON ESCHERICHIA COLI.
Matthew Henry Lum and Steve DeGusta (teacher). John F. Kennedy High School, 6715 Gloria Drive,
Sacramento, CA 95831.
In this experiment, I am trying to find out if Neosporin ointment will inhibit the growth of E. coli. Using
aseptic methods, I streaked E. coli on ten petri dishes containing nutrient agar. Five of the petri dishes were
used as the experiment and the remaining five petri dishes were used as the control. In the each experiment
dish, I placed four self-made antibiotic disks in a straight line, evenly spaced from each other. The self-made
antibiotic disks were plain paper disks that were covered with a thin layer of Neosporin ointment. In each control
dish, I placed four plain paper disks in a straight line even spaced from each other. Then all ten dishes were
placed in the incubator. After incubating the ten dishes at 37°C for 24 hours, the results were that 18 out of the
20 experimental antibiotic disks had a zone of inhibition around them and 20 out of the 20 plain control disks
had no zone of inhibition around them. Results indicated that 18 out of the 20 experimental antibiotic disks
inhibited the growth of E. coli, while 20 out of the 20 plain control disks did not inhibit the growth of E. coli.
Therefore, I conclude Neosporin does inhibit the growth of E. coli. After statistical analysis, my p-value was
less than 0.001, so my conclusion was not due to chance.