SINGLE RP-HPLC METHOD FOR THE ... - Ijsidonline.info

Hari Kishan Reddy Ganthi et al., IJSID, 2012, 2 (4), 471-490Flow rate : 1.0 mL/minTemperature : 30°CRun time : 30minMobile phase : Buffer and Acetonitrile 67:33 v/vPreparation of standard solution: For 100/ 500 / 500 mg Tablets:Accurately weigh and transfer about 100 mg of Aceclofenac working standard into 50 mL volumetric flask, add to it about30 mL of acetonitrile and sonicate to dissolve, dilute up to the mark with acetonitrile and mix well. (Concentration ofAceclofenac is about 2000µg/mL)Accurately weigh and transfer 100 mg of Paracetamol working standard, and 100 mg of Chlorzoxazone working standardin to 200 mL volumetric flask, add to it about 10 mL of above Aceclofenac stock solution and sonicate to dissolve, dilute upto the mark with mobile phase and mix well. (Concentration of Aceclofenac is about 100 µg/mL, concentration ofParacetamol is about 500µg/mL and concentration of Chlorzoxazone is about 500µg/mL ).Preparation of standard solution: For 100/ 325/ 250 mg Tablets:Accurately weigh and transfer about 100 mg of Aceclofenac working standard into 50 mL volumetric flask, add to it about30 mL of acetonitrile and sonicate to dissolve, dilute up to the mark with acetonitrile and mix well. (Concentration ofAceclofenac is about 2000µg/mL)Accurately weigh and transfer 65 mg of Paracetamol working standard, and 50 mg of Chlorzoxazone working standard into 200 mL volumetric flask, add to it about 10 mL of above Aceclofenac stock solution and sonicate to dissolve, dilute up tothe mark with mobile phase and mix well. (Concentration of Aceclofenac is about 100 µg/mL, concentration ofParacetamol is about 325µg/mL and concentration of Chlorzoxazone is about 250µg/mL).Preparation of standard solution: For 100/ 500 mg Tablets:Accurately weigh and transfer about 100 mg of Aceclofenac working standard into 50 mL volumetric flask, add to it about30 mL of acetonitrile and sonicate to dissolve, dilute up to the mark with acetonitrile and mix well. (Concentration ofAceclofenac is about 2000µg/mL)Accurately weigh and transfer 100 mg of Paracetamol working standard, in to 200 mL volumetric flask, add to it about 10mL of above Aceclofenac stock solution and sonicate to dissolve, dilute up to the mark with mobile phase and mix well.(Concentration of Aceclofenac is about 100 µg/mL, concentration of Paracetamol is about 500µg/mL).Preparation of standard solution: For 100/ 500/15 mg Tablets:Accurately weigh and transfer about 100 mg of Aceclofenac working standard into 50 mL volumetric flask, add to it about30 mL of acetonitrile and sonicate to dissolve, dilute up to the mark with acetonitrile and mix well. (Concentration ofAceclofenac is about 2000µg/mL)Accurately weigh and transfer 100 mg of Paracetamol working standard, in to 200 mL volumetric flask, add to it about 10mL of above Aceclofenac stock solution and sonicate to dissolve, dilute up to the mark with mobile phase and mix well.(Concentration of Aceclofenac is about 100 µg/mL, concentration of Paracetamol is about 500µg/mL).Preparation of standard solution: For 100mg Tablets:Accurately weigh and transfer about 100 mg of Aceclofenac working standard into 50.0 mL volumetric flask, add to itabout 30 mL of acetonitrile and sonicate to dissolve, dilute up to the mark with acetonitrile and mix well. (Concentrationof Aceclofenac is about 2000µg/mL)International Journal of Science Innovations and Discoveries, Volume 2, Issue 4, July-August 2012473