SINGLE RP-HPLC METHOD FOR THE ... - Ijsidonline.info

Linearity of Test Method (Inferred from Accuracy):Hari Kishan Reddy Ganthi et al., IJSID, 2012, 2 (4), 471-490The results obtained in accuracy study were further interpreted for the evaluation of linearity of the test method. Theresults of average of mg added at each spiked level was plotted against average mg recovered at each accuracy level and thecorrelation coefficient for set of data was evaluated. The results are tabulated in Table 7.For 100/500/500 mg Tablets:Recovery levelAmountAdded (mg)Table 7: Linearity of Test method (Inferred from accuracy)Aceclofenac Paracetomol ChlorzoxazoneAmountRecovered(mg)AmountAdded(mg)AmountRecovered(mg)AmountAdded(mg)AmountRecovered(mg)80% 79.4 81.0 397.2 401.0 397.1 403.0100% 99.6 101.0 495.8 498.0 495.6 502.0120% 119.6 121.0 594.8 590.0 595.0 594.0CorrelationCoefficient1.000 0.9999 0.9997Slope 0.995 0.9565 0.9651Intercept 1.9627 21.9933 21.0867Precision at Lower and Higher extreme concentrations:Precision at lower and higher extreme range concentration for Aceclofenac (29.9976 µg/ml and 159.9870 µg/ml),Paracetamol (150.4635 µg/ml and 752.3177 µg/ml), and Chlorzoxazone (120.2281 µg/ml and 761.4448 µg/ml) ofLinearity levels were determined as per proposed method by injecting six replicate injections of standards for both thelevels. % RSD for peak responses for both the level was evaluated. The results are tabulated in Table 8.Table 8: Precision at Lower and Higher extreme Levels of linearityArea ResponsesArea Responses Aceclofenac Area Responses ParacetamolChlorzoxazoneInjection NumberLower level Higher level Lower level Higher level Lower level Higher level(25%) (150%) (25%) (150%) (25%) (150%)1 1051198 5593151 2654481 13053921 3607531 224874582 1053062 5599304 2655088 13029744 3605991 224757483 1052477 5593984 2646499 13073632 3602871 225549934 1051003 5598529 2637079 13028568 3600717 224720925 1052376 5602959 2635339 13056944 3598395 225061956 1050552 5616261 2641174 13023193 3606288 22467814Average 1051778 5600698 2644943 13044334 3603632 22494050SD 993.4749 8438.0317 8539.324 20088.1667 3586.7515 32895.1346% RSD 0.1 0.2 0.3 0.2 0.1 0.1Accuracy:An accuracy study was conducted by spiking the known amount of analytes in the equivalent weight of placebo. Accuracystudy was conducted in triplicate at three different levels, (80%, 100% and 120% of target concentration). The sampleswere analyzed as per the proposed test procedure and the % recovery for each spike level was calculated. The precision ateach spike level was also established.The results are tabulated in Table 9, 10 and 11.International Journal of Science Innovations and Discoveries, Volume 2, Issue 4, July-August 2012477