SINGLE RP-HPLC METHOD FOR THE ... - Ijsidonline.info

Hari Kishan Reddy Ganthi et al., IJSID, 2012, 2 (4), 471-490Dilute the 10 mL of above Aceclofenac stock solution to 200 mL with mobile phase and mix well. (Concentration ofAceclofenac is about 100 µg/mL).Preparation of test solution: For 100/ 500 / 500 mg Tablets:Accurately weigh not less than 20 tablets and determine the average weight. Crush the tablets to fine powder. Weighaccurately the powder equivalent to 500 mg of Paracetamol into a 200 mL volumetric flask, add to it 20 ml ofacetonitrile and sonicate to disperse the content. Add to it 130 ml of mobile phase and sonicate to dissolve for 15 minuteswith intermittent shaking. Allow the solution cool to room temperature. Dilute to the volume with mobile phase and mix.Filter the solution through 0.45µ nylon membrane filter. Further dilute 5 mL of the supernatant solution to 25 mL withdiluent.Preparation of test solution: For 100/ 325 / 250 mg Tablets:Accurately weigh not less than 20 tablets and determine the average weight. Crush the tablets to fine powder. Weighaccurately the powder equivalent to 325 mg of Paracetamol into a 200 mL volumetric flask, add to it 20 ml ofacetonitrile and sonicate to disperse the content. Add to it 130 ml of mobile phase and sonicate to dissolve for 15 minuteswith intermittent shaking. Allow the solution cool to room temperature. Dilute to the volume with mobile phase and mix.Filter the solution through 0.45µ nylon membrane filter. Further dilute 5 mL of the supernatant solution to 25 mL withdiluent.Preparation of test solution: For 100/ 500 / 15 mg Tablets:Accurately weigh not less than 20 tablets and determine the average weight. Crush the tablets to fine powder. Weighaccurately the powder equivalent to 500 mg of Paracetamol into a 200 mL volumetric flask, add to it 20 ml ofacetonitrile and sonicate to disperse the content. Add to it 130 ml of mobile phase and sonicate to dissolve for 15 minuteswith intermittent shaking. Allow the solution cool to room temperature. Dilute to the volume with mobile phase and mix.Filter the solution through 0.45µ nylon membrane filter. Further dilute 5 mL of the supernatant solution to 25 mL withdiluent.Prepare the test solutions in duplicate.Procedure:Equilibrate the column with mobile phase for sufficient time until stable baseline is obtained. Inject blank, standard andsample preparation filter through 0.45µ nylon filter. Inject blank (diluent) in single, standard preparation in fivereplicates, and each test preparation into the chromatographic system and record the chromatograms. Inject standardpreparation as a bracketing after every six injections of test preparations. Evaluate the system suitability parameters fromthe standard chromatograms. The order of elution is Paracetamol, Chlorzoxazone and Aceclofenac.Calculation: % content= Test solution area x Std. Dilution factor x Std. PotencyStandard solution area x Test dilution factorRESULTS AND DISCUSSIONSystem suitability:Standard solution was prepared as per the proposed test method and injected into the HPLC system. The results of thesystem suitability assessment for initial validation study parameters were tabulated in Table 1.International Journal of Science Innovations and Discoveries, Volume 2, Issue 4, July-August 2012474