Fluorescence Lifetime Imaging Microscopy Microscopy [FLIM]

Fig. 7 Example confocal images of TPE-PDT using porphyrin dimer sensitiser P 2 C 2 -NMeI on SK-OV-3 cells; only the centralsquare region, indicated by the white box (230×230 µm), was irradiated. The combined transmission and fluorescence imagesare shown, all nuclei are stained with Hoechst 33258 (blue) and cells with compromised plasma membranes are co-stained withSytox orange (magenta). The central region was irradiated with 920 nm (300 fs, 90 MHz, 6.8 mW: (a) 60 scans, (b) 100 scansand (c) 320 scans.