Fluorescence Lifetime Imaging Microscopy Microscopy [FLIM]

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The structure of GreenGFluorescentProtein(GFP) and its fluorescent centre•Originally from jellyfish•Gene can be expressed in any organism•Fluorescently label a specific proteinfluorophoreprotected by thebarrel structure40 Å30 Å
Page 1 and 2: Fluorescence Lifetime ImagingMicros
Page 5: Gated Fluorescence Imaging1.210.80.
Page 9 and 10: TCSPC FLIM
Page 11 and 12: • Drug non toxic inabsence of lig
Page 14: Busal cell C
Page 18 and 19: V-49-4 4 cells• longer lived comp
Page 21 and 22: PDT Experiment: Drug + laser (avera
Page 23 and 24: Fig. 6 One-photon effect on SK-OV-3
Page 28 and 29: Imaging Local RefractiveIndex
Page 30: Fluorescence Lifetime, τ, ofGFP va
Page 33 and 34: 3D FLIM of MHC-GFP2.8nsMicroscopy o
Page 36 and 37: Fluorescence lifetime of GFP-tagged
Page 38 and 39: KIR’s‣ KIR phosphorylation afte
Page 40 and 41: Fluorescence Resonance EnergyTransf
Page 42 and 43: Imaging KIR2DL1 phosphorylation byF
Page 44 and 45: Anisotropy reports tumbling of mole
Page 46 and 47: A schematic of the polarization-res
Page 48 and 49: Time-resolved fluorescence anisotro
Page 50 and 51: a) r(t 1 ) b) θ ∼ η c)0.410ns 5
Page 52 and 53: Rotational relaxation in Live Cells
Page 54 and 55: Figure 1Emission intensity / arb. u
Page 56 and 57: Fluorescence quantum yield, ΦFluor
Page 58 and 59: Figure 5(a)(b)Figure 6(a)(b)2200 ps
Page 60: Acknowledgements‣ Paul French, Ph

Fluorescence Lifetime Imaging Microscopy Microscopy [FLIM]

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