Fluorescence Lifetime Imaging Microscopy Microscopy [FLIM]

vertical excitationhorizontal excitationa)L-formatI ⊥I ⊥I ||I ⊥b)collinearI ⊥I || I ||Fig. 4: The polarization of the fluorescence emission for different set-ups. (a) The geometry of anL-format set-up for horizontal and vertical excitation. Note that for horizontal polarization of theexcitation beam, the emission polarization is perpendicular for both horizontal and verticalemission polarizer settings, which allows the G-factor to be determined as the ratio of bothmeasured intensities. (b) The geometry for a collinear set-up where the rotation of the polarizationof the excitation also results in a rotation of the polarization of the emission.I ⊥