Fluorescence Lifetime Imaging Microscopy Microscopy [FLIM]

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KIR’s‣ KIR phosphorylation after engagement ofMHC class 1 protein on target cells isdifficult to detect biochemically [maybeonly small fraction of KIR’s phosphorylatedat any given time]‣ KIR’s and corresponding MHC Class 1proteins cluster at immunological synapse‣ Study by imaging KIR phosphorylation atthe NK/ cell IS using FRET/<strong>FLIM</strong>
<strong>FLIM</strong> of FRET to probe NK cell inhibitorysignallingKIR2DL1
Page 1 and 2: Fluorescence Lifetime ImagingMicros
Page 5: Gated Fluorescence Imaging1.210.80.
Page 9 and 10: TCSPC FLIM
Page 11 and 12: • Drug non toxic inabsence of lig
Page 14: Busal cell C
Page 18 and 19: V-49-4 4 cells• longer lived comp
Page 21 and 22: PDT Experiment: Drug + laser (avera
Page 23 and 24: Fig. 6 One-photon effect on SK-OV-3
Page 27 and 28: The structure of GreenGFluorescentP
Page 29 and 30: Strickler-Berg formula×−92 ∫1
Page 32 and 33: The Immune Synapse• Facilitates i
Page 34: The Missing Self Hypothesis
Page 37: NK Cells‣ Activation regulated by
Page 41 and 42: Phosphorylation of the NK cell inhi
Page 43 and 44: Summary‣ FLIM is a robust techniq
Page 45 and 46: vertical excitationhorizontal excit
Page 47 and 48: Molecular tumbling measured bytime-
Page 49 and 50: θ =ηVkT=> molecular radius of flu
Page 51 and 52: methanolethyleneglycolmethanolethyl
Page 53 and 54: Scheme 1O(1)NB F 2NO(2)NB F 2N
Page 55 and 56: Figure 4τ -1 / ns -10.360.340.320.
Page 57 and 58: Probes in cells‣ SL-OVOV-3 3 cell
Page 59 and 60: Figure 90.250.20Anisotropy, r0.150.

Fluorescence Lifetime Imaging Microscopy Microscopy [FLIM]

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