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Paramecium - Deutsche Gesellschaft für Protozoologie / German ...

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Niche separation and coexistence of chrysophytes: The<br />

Alpine Lake Gradient Analysis (ALGA)<br />

Jens Boenigk<br />

Institut <strong>für</strong> Limnologie der Österreichischen Akademie der Wissenschaften<br />

We currently investigate microeukaryotes with a focus on chrysomonad<br />

flagellates in an alpine gradient comprising 40 lakes. The analysis<br />

comprises morphological surveys based on light and electron microscopy<br />

as well as molecular surveys. We further isolated more than<br />

100 chrysophyte strains from these lakes. For these strains we investigate<br />

molecular signatures as well as the ecophysiological adaptation.<br />

Resting stages, i.e. cysts, are analysed from material captured in sediment<br />

traps and linked to the vegetative cells by means of molecular<br />

methods. Both, chrysomonad cysts as well as vegetative cells are analysed<br />

on a seasonal basis for selected lakes. Aspects of the microdiversity<br />

and population dynamics are covered by cultivation-independent<br />

molecular methods. Our main goal is to gain insights in the<br />

coexistence, niche differentiation and distribution pattern of the target<br />

organisms by a holistic approach combing molecular, morphological,<br />

ecophysiological and palaeolimnological data. We will present an<br />

overview on the current activities and first results.<br />

jens.boenigk@oeaw.ac.at<br />

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