Cardiac Regeneration and Cell Therapy - Herzzentrum Uniklinik Koeln
Cardiac Regeneration and Cell Therapy - Herzzentrum Uniklinik Koeln
Cardiac Regeneration and Cell Therapy - Herzzentrum Uniklinik Koeln
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Speakers <strong>and</strong> Chairpersons<br />
(in alphabetical order)<br />
Atta Behfar<br />
Mayo Clinic Center for Regenerative Medicine<br />
Rochester, MN, USA<br />
Jolanta Chmielowiec<br />
Cardiovascular Research Center, Massachusetts General Hospital<br />
Boston, MA, USA<br />
Yeong-Hoon Choi<br />
<strong>Uniklinik</strong>en Cologne, Dept. of Cardiothoracic Surgery<br />
Cologne, Germany<br />
Douglas B. Cowan<br />
Children’s Hospital Boston <strong>and</strong> Harvard Medical School, Dept. of Anesthesiology<br />
Boston, MA, USA<br />
Frank Edenhofer<br />
Institute of Reconstructive Neurobiology, Life & Brain Center<br />
University of Bonn, Bonn, Germany<br />
Bernd Fleischmann<br />
Institute for Physiology I, Life & Brain Center,<br />
University of Bonn, Bonn, Germany<br />
Clifford Folmes<br />
Mayo Clinic Center for Regenerative Medicine<br />
Rochester, MN, USA<br />
Wolfgang Franz<br />
Ludwig-Maximilians University, Dept. of Cardiology<br />
Munich, Germany<br />
Lior Gepstein<br />
Technion - Israel Institute of Technology, Rappaport Institute<br />
Dept. of Physiology <strong>and</strong> Biophysics, Haifa, Israel<br />
Marie-Jose Goumans<br />
Leiden University Medical Center<br />
Leiden, Netherl<strong>and</strong>s<br />
Kaomei Guan<br />
Heart Center, University of Göttingen<br />
Göttingen, Germany<br />
Roger J. Hajjar<br />
The Mount Sinai Medical Center<br />
New York, NY, USA<br />
Marcel Halbach<br />
<strong>Uniklinik</strong>en Cologne, Dept. of Cardiology<br />
Cologne, Germany<br />
Kevin Healy<br />
University of California, Dept. of Bioengineering<br />
Berkeley, CA, USA<br />
Stefan Herzig<br />
<strong>Uniklinik</strong>en Cologne, Dept. of Pharmacology<br />
Cologne, Germany<br />
Jürgen Hescheler<br />
Institute for Neurophysiology, University of Cologne<br />
Cologne, Germany<br />
Masaki Ieda<br />
Keio University School of Medicine<br />
Tokyo, Japan<br />
Szuszanna Izsvak<br />
Max Delbrück Center for Molecular Medicine<br />
Berlin, Germany<br />
Stefan Janssens<br />
Vesalius Research Center, Katholic University of Leuven<br />
Leuven, Belgium<br />
Markus Khalil<br />
<strong>Uniklinik</strong>en Cologne, Dept. of Pediatric Cardiology<br />
Cologne, Germany<br />
Benjamin Krausgrill<br />
<strong>Uniklinik</strong>en Cologne, Dept. of Cardiology<br />
Cologne, Germany<br />
Linda van Laake<br />
Heart Lung Center Utrecht<br />
Utrecht, Netherl<strong>and</strong>s<br />
Dennis Ladage<br />
<strong>Uniklinik</strong>en Cologne, Dept. of Cardiology<br />
Cologne, Germany<br />
Jonathan Leor<br />
Sheba Medical Center, Neufeld <strong>Cardiac</strong> Research Institute, Tel-Aviv University<br />
Tel Aviv, Israel<br />
John Listello<br />
Sigma-Aldrich, St.Louis, USA<br />
Christina Mauritz<br />
LEBAO - Leibniz Research Laboratories for Biotechnology <strong>and</strong> Artificial Organs,<br />
Hannover Medical School, Hannover, Germany<br />
Alex<strong>and</strong>er Meissner<br />
Broad Institute, Harvard Stem <strong>Cell</strong> Institute <strong>and</strong> Department of Stem <strong>Cell</strong> <strong>and</strong> Regenerative<br />
Biology, Harvard University, Cambridge, MA, USA<br />
Marc Mercola<br />
Burnham Institute for Medical Research<br />
La Jolla, CA, USA<br />
Antoon F. Moorman<br />
Heart Failure Research Center<br />
Amsterdam, Netherl<strong>and</strong>s<br />
Martin Morad<br />
Medical University of South Carolina, <strong>Cell</strong> Biology <strong>and</strong> Anatomy<br />
Charleston, SC, USA<br />
Christine Mummery<br />
Leiden University Medical Centre<br />
Leiden, Netherl<strong>and</strong>s<br />
Jochen Müller-Ehmsen<br />
<strong>Uniklinik</strong>en Cologne, Dept. of Cardiology<br />
Cologne, Germany<br />
Klaus Neef<br />
<strong>Uniklinik</strong>en Cologne, Dept. of Cardiothoracic Surgery<br />
Cologne, Germany<br />
Barbara Pfüller<br />
Berlin-Br<strong>and</strong>enburg Center for Regenerative <strong>Therapy</strong> (BCRT)<br />
Berlin, Germany<br />
Frank Pillekamp<br />
Heinrich Heine University Düsseldorf, Clinic for Pediatric Cardiology <strong>and</strong> Pulmonology<br />
Düsseldorf, Germany<br />
Michael Roberts<br />
University of Missouri, Dept. of Biochemistry<br />
Columbia, MU, USA<br />
Philip Sasse<br />
Institute for Physiology I, Life & Brain Center, University of Bonn<br />
Bonn, Germany<br />
Tomo Saric<br />
Institute for Neurophysiology, University of Cologne,<br />
Cologne, Germany<br />
Dinender Singla<br />
Burnett School of Biomedical Sciences, University of Central Florida<br />
Orl<strong>and</strong>o, FL, USA<br />
Christof Stamm<br />
Berlin-Br<strong>and</strong>enburg Center for Regenerative <strong>Therapy</strong> (BCRT)<br />
Berlin, Germany<br />
Andre Terzic<br />
Mayo Clinic Center for Regenerative Medicine<br />
Rochester, MN, USA<br />
Gordana Vunjak-Novakovic<br />
Columbia University, Dept. of Biomedical Engineering<br />
New York, NY, USA<br />
Thorsten Wahlers<br />
<strong>Uniklinik</strong>en Cologne, Dept. of Cardiothoracic Surgery<br />
Cologne, Germany<br />
Joseph C. Wu<br />
Institute for Stem <strong>Cell</strong> Biology <strong>and</strong> Regenerative Medicine, Stanford University School of<br />
Medicine, Stanford, CA, USA<br />
Wolfram Zimmermann<br />
University Medical Center, Institute of Pharmacology<br />
Göttingen, Germany<br />
Robert Zweigerdt<br />
LEBAO - Leibniz Research Laboratories for Biotechnology <strong>and</strong> Artificial Organs<br />
Hannover Medical School, Hannover, Germany<br />
Cologne Conference on<br />
<strong>Cardiac</strong> <strong>Regeneration</strong> <strong>and</strong><br />
<strong>Cell</strong> <strong>Therapy</strong><br />
May 13-14, 2011<br />
Cologne, Germany<br />
Hotel Azimut Köln, Hansaring 97<br />
Symposium of the<br />
BMBF-Consortium<br />
“Stem <strong>Cell</strong> <strong>Therapy</strong> of the Heart”<br />
12 CME Points requested<br />
Scientific Coordinators:<br />
Jochen Müller-Ehmsen<br />
Tomo Saric<br />
Yeong-Hoon Choi<br />
Rol<strong>and</strong> Adelmann<br />
Stefan Herzig<br />
RAMSES<br />
Dear Colleagues <strong>and</strong> Friends,<br />
Welcome to the 1 st Cologne Conference on <strong>Cardiac</strong><br />
<strong>Regeneration</strong> <strong>and</strong> <strong>Cell</strong> <strong>Therapy</strong> 2011. Heart failure <strong>and</strong><br />
underlying diseases are the most frequent cause of death in<br />
adults worldwide. In recent years, cell-based tissue repair<br />
approaches have been extensively explored as a promising<br />
alternative to existing pharmacological <strong>and</strong> surgical therapies<br />
for these conditions. Different types of stem cells of adult <strong>and</strong><br />
embryonic origin have been tested in animal models as<br />
c<strong>and</strong>idates for cardiac cell replacement therapy. Adult stem<br />
cells derived from bone marrow were among the first to enter<br />
clinical trials but their therapeutic potency is still a matter of<br />
debate. Embryonic stem cells have been regarded as a<br />
valuable source of therapeutic cells. However, unresolved<br />
ethical concerns, their allogeneic nature <strong>and</strong> variable yield of<br />
differentiated cells seriously hampered their use for human<br />
therapy. With emergence in recent years of autologous <strong>and</strong><br />
ethically less controversial induced pluripotent stem cells, the<br />
hope that they may be more suitable for clinical use increased<br />
dramatically.<br />
However, despite these ground-breaking achievements the<br />
development of new cell-based treatment strategies will<br />
require further research <strong>and</strong> concerted action of different<br />
scientific disciplines ranging from molecular, cell <strong>and</strong><br />
developmental biology, immunology, radiology, surgery,<br />
biotechnology, bioengineering to chemistry, physics, ethics<br />
<strong>and</strong> regulatory affairs. Only in this way it may be possible to<br />
produce sufficient amounts of pure, safe, affordable <strong>and</strong><br />
therapeutically potent stem cell derivatives for the benefit of<br />
patients suffering from heart disease. Future research in this<br />
extremely dynamic <strong>and</strong> multidisciplinary field will determine<br />
whether the regenerative approach will be part of evidence<br />
based cardiac therapy in the future.<br />
The purpose of this conference is to bring together basic<br />
scientists <strong>and</strong> clinicians with different backgrounds <strong>and</strong><br />
expertises in order to exchange ideas <strong>and</strong> discuss new<br />
developments in this important research area. The meeting<br />
focuses on cell-based approaches for cardiac repair <strong>and</strong><br />
issues that must be addressed to allow their clinical<br />
translation. We hope that you will benefit from this conference<br />
<strong>and</strong> wish you a pleasant stay in Cologne.<br />
Yours sincerely,<br />
Tomo Saric Jochen Müller-Ehmsen Yeong-Hoon Choi
Friday, May 13 th , 2011<br />
9:00 – 9:10 Welcome address, M. Paulsson<br />
Associate Dean (Research) of the Medical Faculty<br />
9:10 – 9:20 Welcome address, J. Hescheler<br />
Speaker of the BMBF consortium<br />
Session 1: Embryonic Heart Development<br />
Chairs: C. Mummery, J. Hescheler<br />
9:20 – 9:50 Jolanta Chmielowiec<br />
Chemical screening <strong>and</strong> iPS cell model systems define role of TGF-β<br />
signaling in islet heart progenitors<br />
9:50 – 10:20 Marc Mercola<br />
Natural <strong>and</strong> synthetic inducers of heart differentiation<br />
10:20 – 10:50 Antoon F. Moorman<br />
<strong>Cardiac</strong> growth<br />
Coffee Break 10:50 – 11:20<br />
Session 2: Sources of Stem <strong>Cell</strong>s for <strong>Cardiac</strong> Repair I<br />
Chairs: M. Roberts, T. Saric<br />
11:20 – 11:40 Wolfram Zimmermann<br />
Parthenogenetic stem cells for cardiac repair<br />
11:40 – 12:00 Kaomei Guan<br />
Pluripotent germ line stem cells for cardiac repair<br />
12:00 – 12:20 Dinender Singla<br />
TGF-β2-primed ES cells in cardiac repair <strong>and</strong> regeneration<br />
12:20 – 12:40 Marie-Jose Goumans<br />
Human cardiomyocyte progenitor cells: Maturation <strong>and</strong> functional<br />
impact<br />
12:40 – 13:00 Wolfgang Franz<br />
Forward programming of pluripotent stem cells towards<br />
cardiomoycytes<br />
Lunch Break 13:00 -14:00<br />
Session 3: Sources of Stem <strong>Cell</strong>s for <strong>Cardiac</strong> Repair II<br />
Chairs: D. Singla, J. Müller-Ehmsen<br />
14:00 – 14:20 Christine Mummery<br />
Opportunities <strong>and</strong> challenges in using stem cells for cardiac repair<br />
14:20 – 14:40 Clifford Folmes/Andre Terzic<br />
Metabolic dynamics in nuclear reprogramming<br />
14:40 – 15:00 Michael Roberts<br />
iPS cells from swine: Potential for cardiac repair in a large animal<br />
model<br />
15:00 – 15:20 Robert Zweigerdt<br />
Scalable differentiation of pluripotent stem cells towards<br />
cardiomyocytes<br />
15:20 – 15:40 Masaki Ieda<br />
A new method for cardiac regeneration: Direct conversion of<br />
fibroblasts into cardiomyocytes by defined factors<br />
Coffee Break 15:40 – 16:10<br />
Session 4: Properties of Stem <strong>Cell</strong> Derived Cardiomyocytes<br />
Chairs: B. Fleischmann, S. Herzig<br />
16:10 – 16:25 Marcel Halbach<br />
Integration <strong>and</strong> maturation of cells<br />
16:25 – 16:40 Tomo Saric<br />
Transcriptional profiles of ES <strong>and</strong> iPS cell derived cardiomyocytes<br />
16:40 – 17:05 Martin Morad<br />
Maturation of calcium h<strong>and</strong>ling in cardiomyocytes during development<br />
17:05 – 17:30 Lior Gepstein<br />
Transplantation of a tissue-engineered human vascularized cardiac<br />
muscle<br />
17:30 – 17:55 Linda van Laake<br />
Comparison of murine ES <strong>and</strong> iPS cell-derived cardiomyocytes<br />
17:55 – 18:10 Markus Khalil<br />
Contractile behavior of native murine ventricular tissue <strong>and</strong><br />
cardiomyocytes derived from ES or iPS cells<br />
19:30 Dinner<br />
Saturday, May 14 th , 2011<br />
Session 5: (Epi)genetic Modifications of Therapeutic <strong>Cell</strong>s<br />
Chairs: M. Mercola, J. C. Wu<br />
9:00 – 9:25 Alex<strong>and</strong>er Meissner<br />
Epigenetic modifications in pluripotent <strong>and</strong> differentiated cells<br />
9:25 – 09:50 Frank Edenhofer<br />
Engineering cell-permeant FLP recombinase for tightly controlled<br />
inducible <strong>and</strong> reversible overexpression in ES cells<br />
09:50 – 10:15 John Listello<br />
Zinc finger nuclease (ZFN)-Engineering of iPS cells for cardiac<br />
specific lineage tags<br />
10:15 – 10:40 Szuszanna Izsvak<br />
Transposons as tools for genetic modification of stem cell derivatives<br />
Coffee Break 10:40 – 11:10<br />
Session 6: Tissue Engineering / <strong>Cell</strong> Delivery<br />
Chairs: W. Zimmermann, Y.-H. Choi<br />
11:10 – 11:35 Gordana Vunjak-Novakovic<br />
Scaffold stiffness <strong>and</strong> functional properties of 3D cardiac constructs<br />
11:35 – 12:00 Doug Cowan<br />
<strong>Cell</strong>-based therapy for pediatric heart block<br />
12:00 – 12:25 Kevin Healy<br />
Two-photon-initiated polymerization for creation of 3D scaffolds for<br />
tissue engineering<br />
12:25 – 12:50 Jonathan Leor<br />
Biomaterials <strong>and</strong> the efficacy of cell therapy<br />
12:50 – 13:00 Benjamin Krausgrill<br />
In vivo tissue engineering<br />
Lunch Break 13:00 – 14:00<br />
Session 7: In vivo Imaging of Grafted <strong>Cell</strong>s/Large Animal Models<br />
Chairs: G. Vunjak-Novakovic, F. Pillekamp<br />
14:00 – 14:15 Klaus Neef<br />
Serial in vivo cell tracking <strong>and</strong> evaluation of cell therapeutic effects by<br />
MRI<br />
14:15 – 14:40 Joseph C. Wu<br />
Pluripotent stem cells: biological insights from molecular imaging<br />
14:40 – 15:05 Philip Sasse<br />
Optogenetic control of heart muscle in vitro <strong>and</strong> in vivo<br />
15:05 – 15:20 Dennis Ladage<br />
Pre-clinical large animal studies on cardiac regeneration<br />
Coffee Break 15:20 – 15:45<br />
Session 8: Pre-Clinical <strong>and</strong> Clinical Approaches to <strong>Cardiac</strong><br />
<strong>Regeneration</strong><br />
Chairs: A. Terzic, T. Wahlers<br />
15:45 – 16:10 Roger J. Hajjar<br />
In vivo myocardial distribution of multipotent progenitor cells following<br />
intracoronary delivery in a swine model of myocardial infarction<br />
16:10 – 16:30 Christof Stamm<br />
Clinical studies on cardiac repair with adult stem cells - the surgeon’s<br />
view<br />
16:30 – 16:50 Stefan Janssens<br />
Clinical studies on cardiac repair with adult stem cells - the<br />
cardiologist’s view<br />
16:50 – 17:15 Atta Behfar/Andre Terzic<br />
Lineage-specified cardiopoietic stem cell therapy for heart failure<br />
17:15 – 17:35 Christina Mauritz<br />
iPS cell-derived Flk-1 progenitor cells engraft, differentiate <strong>and</strong><br />
improve heart function in a mouse model of acute myocardial<br />
infarction<br />
17:35 – 18:00 Barbara Pfüller<br />
Obstacles to meet in the bench-to-bedside translation<br />
18:00 – 18:10 Concluding Remarks
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