prvi kongres hrvatskih znanstvenika iz domovine i inozemstva - HATZ

PRVI KONGRES HRVATSKIH ZNANSTVENIKA IZ DOMOVINE I INOZEMSTVAZagreb - Vukovar, 15. - 19. studenog 2004.P - 32CORRELATION OF BIOMETRIC PARAMETERS WITHMETALLOTHIONEIN AND CYTOSOLIC METAL LEVELS (Zn, Cu, Cd)IN LIVER, KIDNEY AND BRAIN OF Mullus surmuletus AND Lizaaurata FROM THE EASTERN ADRIATIC SEAFilipović, Vlatka; Raspor, BiserkaRuñer Bošković Institute, Zagreb, CroatiaThe present study was initiated as the collaborative project with Norwegian Institute forWater Research to define the fluctuations of metallothionein (MT) and metal levels inliver, kidney and brain of two wild fish species, representative for the Eastern AdriaticSea. The studied marine area (Kastela Bay) is affected by industrial and domesticwastewater inputs. Fish tissues of Mullus surmuletus (striped red mullet), benthic speciesand Liza aurata (golden gray mullet), pelagic species, were homogenised and centrifugedat 50000xg to get cytosolic fraction (S50) which was appropriately diluted, heat-treatedand analysed on MT and metal (Zn, Cu, Cd) content. MTs were analysed by differentialpulse polarography and metals by flame atomic absorption spectrometry. The resultsshow species and tissue dependence. Highest MT and metal levels are determined inliver of Liza aurata (0.39± ; ; 0.06 milligram MT/millilitre S50 ; 6.46± ; ; 3.45 microgramCu/millilitre S50) and in kidney of Mullus surmuletus (0.57± ; ; 0.15 milligramMT/millilitre S50 ; 1.13 ; ; 0.50 microgram Zn/millilitre S50). Our results support the role ofliver and kidney as main detoxification organs. Correlation between metals and MTs isstatistically significant for essential metals, especially Cu (0.69) in Liza aurata and Zn(0.95) in Mullus surmuletus suggesting that all metal binding sites of MT are not occupiedyet. Total length, body weight and age are highly correlated biometric parameters withMT and soluble metal levels, which show increasing trend in liver of both species,indicating possible accumulation during life stage. These results emphasise theimportance of considering additional factors, such as biometric parameters, in the surveyof endogenous and exogenous factors, which are involved in cellular effects of metals.Presented on:PRIMO 12Tampa, Florida, SAD, 09.-13.05.2003.Principal Investigator : Biserka RasporProject No. 009813034