LSM 510 Laser Scanning Microscope

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2.8 Microscopes LSM 510 - SETUP REQUIREMENTS Laser Specifications LSM 510 Inverted Axiovert 100 M BP or SP Upright Axioplan 2 MOT All Zeiss ICS objectives and accessories can be accommodated. Z motor DC servomotor, opto-electronically coded Least Z interval: 100 nm HRZ-200 Galvanometer-driven precision focusing stage Max. travel 200 µm; resolution 6 nm; accuracy 40 nm Allows continuous Z-scan at up to 10 Hz 2.9 Scanning Module 2 individually driven galvanometric scanners Scanning speed Up to 2.6 frames/sec (512 x 512 pixels) Field resolution Max. 2048 x 2048 pixels (individually adjustable for each axis) Field of view 10 x 10 mm² with a 1.25x objective Zoom 1x ... 8x, continuous control Channels Up to 4 channels simultaneously 4 confocal reflection/fluorescence channels (PMT) 1 transmitted light channel (PMT) 1 reference monitor diode Cooled PMTs (option, forthcoming) Fiber-optic adaptation of external detectors (option, forthcoming) Dynamic range 12-bit DAC for each detection channel Pinholes 4 individual variable pinholes (for each confocal channel) Computer controlled automatic alignment 2-10 B 40-051 e 07/98
LSM 510 - SETUP REQUIREMENTS LSM 510 Microscopes / Scanning Module 2.10 Laser Module VIS Single-mode polarization preserving fiber Laser beam attenuation for all lasers by VIS-AOTF HeNe laser (543 nm, 1 mW) HeNe laser (633 nm, 5 mW) Ar laser (458, 488, 514 nm, 25 mW) Ar laser (488 nm, 15 mW) ArKr laser (488, 568 nm, 30 mW) Fuses and automatic circuit breakers for 230 V: G-type fuse 5 x 20 mm; slow-blow 3.15 A / H / 250 V, acc. to IEC 127 2 circuit breakers; C 10 A for 110 V: G-type fuse 5 x 20 mm; slow-blow 3.15 A / H / 250 V, acc. to IEC 127 Circuit breaker; B 25 A Circuit breaker; C 25 A Circuit breaker; B 16 A Circuit breaker; B 10 A 2.11 Laser Module UV Single-mode polarization preserving fiber Laser beam attenuation for all lasers by UV-AOTF Ar laser (351, 364 nm, 80 mW) B 40-051 e 07/98 2-11
Page 1 and 2: I LSM 510 Laser Scanning Microscope
Page 3 and 4: LSM 510 INTRODUCTION Developed in C
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Page 7 and 8: LSM 510 INTRODUCTION Kodak XLS 8650
Page 9 and 10: LSM 510 1 2 3 4 5 Notes on Device S
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Page 35 and 36: INTRODUCTION TO LASER SCANNING MICR
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OPERATION LSM 510 Overview of Menu
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OPERATION LSM 510 Image Acquisition
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OPERATION LSM 510 Routine Mode 4.6
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OPERATION LSM 510 Routine Mode 4.6.
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OPERATION LSM 510 Routine Mode 4.6.
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OPERATION LSM 510 Routine Mode �
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OPERATION LSM 510 Routine Mode �
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OPERATION LSM 510 Image Optimizatio
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OPERATION LSM 510 Analysis of Image
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OPERATION LSM 510 Database / Loadin
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OPERATION LSM 510 Macro 4.10 Macro
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OPERATION LSM 510 Macro 4.10.3 Reco
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OPERATION LSM 510 Macro 4.10.5 Edit
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OPERATION LSM 510 Shut-Down Procedu
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OPERATION LSM 510 Shut-Down Procedu
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OPERATION LSM 510 Annex Application
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OPERATION LSM 510 Annex 4.12.2 Filt
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OPERATION LSM 510 Annex Fig. 4-171
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OPERATION LSM 510 Annex 4.12.4.3 Op
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OPERATION LSM 510 Annex � Clickin
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OPERATION LSM 510 Annex 4.12.5 Scan
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OPERATION LSM 510 Annex Moves �
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OPERATION LSM 510 Annex The Current
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OPERATION LSM 510 Annex 4.12.6 Spec
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OPERATION LSM 510 Annex Pin assignm
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OPERATION LSM 510 Annex � Change
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OPERATION LSM 510 Annex To use the
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OPERATION LSM 510 Annex The single
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OPERATION LSM 510 Annex Gain and of
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INTRODUCTION TO LASER SCANNING MICR
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LSM 510 Laser Scanning Microscope

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