LSM 510 Laser Scanning Microscope

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INTRODUCTION TO LASER SCANNING MICROSCOPY Performance Features of the LSM 510 LSM 510 (3) Transmitted-light illumination a) The illuminator support contains a security circuit, which activates a shutter preventing laser light from reaching the stand when the support is moved to back. A complementary shutter built-in the stand prevents laser light from reaching the eye pieces during scanning mode. b) The illuminator support is equipped with a rotatable polarizer. The Axiovert description contains the adjustment for DIC mode during conventional observation. For scanning transmitted light DIC mode the polarizer in the transmitted light support works like an analyzer and must be adjusted in such a manner, that direct laser light will be blocked. The conventional analyzer slider in the stand is not allowed be located in the beam path because of the laser light already is polarized. c) On the illuminator support as an option there is mounted a LSM 5 software controlled switching mirror fully motorized. Alternatively the light is directed to the LSM 5 T-light detector or enables conventional transmitted-light observation. d) The focusing screen for conventional transmitted-light is located in a support in front of the halogen lamp housing. e) Further information to halogen lamp and condensers you will find in the Axiovert operating manual. (4) Reflected light fluorescence All Axiovert fluorescence accessories exceptional the reflector slider can be used. Further information you will find in the Axiovert operation manual. (5) Imaging optics Optovar sliders are not usable. The analyzer for conventional DIC mode will be operated from the right side and is located just below the nosepiece. Use of sliders with auxiliary objects (473704/14) is not possible. (6) Photo equipment The stand doesn´t have an integrated SLR-port, but microscope cameras, as described in the Axiovert operation manual, can be used. (7) TV adaption The TV port aside and the tubes can be used as described in the Axiovert operation manual. The TV interface side port or base port can only be used with TV adapters 44. 3-10 B 40-051 e 07/98
INTRODUCTION TO LASER SCANNING MICROSCOPY LSM 510 Performance Features of the LSM 510 3.4.3 Computer hardware and software The LSM 510 is controlled through a standard high-end Pentium PC. Linking with the electronic control system is via an ultrafast SCSi interface. The PC comes with the 32-bit WINDOWS NT 4.0 operating system. The instrument is fully motorized, permitting fast change-over between methods as well as automatic operation. Parameters once set or complex examination sequences once established can be saved and reproduced; this way, complete application programs can be loaded and executed by pushbutton control. The software of the LSM 510 has two levels. On the simple operator interface level, a result will be achieved after a few prompts; graphical prompting of the user in conjunction with automatic setting of many parameters is an ideal tool for daily routine jobs. The expert level offers perfect facilities for individual settings of functions and parameters. Conversion of the light signals into a digital image is effected by means of four 12-bit A/D converters, each of which can generate 4096 brightness levels. The software provides a enormously wide range of image processing functions, including all standard 2D/3D (stereo, projection) functions same as sophisticated 3D reconstruction capabilities (surface and alpha rendering), digital processing of voxels and 3D measurement functions (surface areas, volumes). As all files and images are recorded in MS Access databases, elegant image database editing is just as easy as transferring the records to other programs. B 40-051 e 07/98 3-11
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OPERATION LSM 510 Image Acquisition
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OPERATION LSM 510 Routine Mode 4.6
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OPERATION LSM 510 Routine Mode 4.6.
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OPERATION LSM 510 Routine Mode 4.6.
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OPERATION LSM 510 Routine Mode �
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OPERATION LSM 510 Routine Mode �
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OPERATION LSM 510 Image Optimizatio
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OPERATION LSM 510 Analysis of Image
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OPERATION LSM 510 Database / Loadin
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OPERATION LSM 510 Macro 4.10 Macro
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OPERATION LSM 510 Macro 4.10.3 Reco
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OPERATION LSM 510 Macro 4.10.5 Edit
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OPERATION LSM 510 Shut-Down Procedu
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OPERATION LSM 510 Shut-Down Procedu
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OPERATION LSM 510 Annex Application
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OPERATION LSM 510 Annex 4.12.2 Filt
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OPERATION LSM 510 Annex Fig. 4-171
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OPERATION LSM 510 Annex 4.12.4.3 Op
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OPERATION LSM 510 Annex � Clickin
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OPERATION LSM 510 Annex 4.12.5 Scan
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OPERATION LSM 510 Annex Moves �
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OPERATION LSM 510 Annex The Current
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OPERATION LSM 510 Annex 4.12.6 Spec
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OPERATION LSM 510 Annex Pin assignm
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OPERATION LSM 510 Annex � Change
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OPERATION LSM 510 Annex To use the
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OPERATION LSM 510 Annex The single
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OPERATION LSM 510 Annex Gain and of
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INTRODUCTION TO LASER SCANNING MICR
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LSM 510 Laser Scanning Microscope

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