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Basics of analysis• Screening• Confirmation• Separation• Detection• Quantification
Assay Methods• Spot & spectrochemical• Immunoassays• Non-competitive• Competitive• Enzyme-multiplied immunoassay (EMIT)• Magnetic microparticle chemiluminescentcompetitive immunosassay• Microparticle capture immunoassay
Page 2 and 3: Special Thanks!• Howard Greller
Page 4 and 5: Laboratories• Clinical Laboratory
Page 7: Magic Answer Box?• Low volume, of
Page 11: Testing“You’re fired, Jack. The
Page 14 and 15: Spectrochemical• Sophisticated sp
Page 16 and 17: Spectrochemical• Older tests wait
Page 18 and 19: Spectrochemical• Improve Selectiv
Page 20 and 21: Immunoassays• Non-competitive•
Page 26: Competitive“Inverse” assay
Page 30 and 31: Immunoassays• Nonisotopic immunoa
Page 32 and 33: EMITGoldfranks 9th edition
Page 34 and 35: ChromatographySeparation and Detect
Page 36 and 37: Thin LayerChromatography• Extract
Page 38 and 39: Thin LayerChromatography• Fast, e
Page 40 and 41: High-PerformanceLiquid Chromatograp
Page 42 and 43: High-PerformanceLiquid Chromatograp
Page 44 and 45: Gas Chromatography• Similar to HP
Page 46 and 47: Gas Chromatography• Substances ha
Page 48 and 49: Mass Spectrometry
Page 50 and 51: LC/MS/MS• High sensitivity and sp
Page 52 and 53: Relative ComparisonMethod Speed Cos
Page 54 and 55: Drug Screens
Page 56 and 57: Cocaine• Highly specific• Benzo
Page 58 and 59:
The “second handsmoking” defens
Page 60 and 61:
Opioids• Morphine, codeine,heroin
Page 62 and 63:
ChallengesDextromethorphan• Dextr
Page 64 and 65:
PCP• Phencyclidine• False Posit
Page 66 and 67:
Amphetamines• Amphetamine assay p
Page 68 and 69:
TCA Challenges• Cross react with
Page 70 and 71:
Adulteration• 2004: First mandato
Page 72 and 73:
In Vivo Adulteration“Ingest prior
Page 74 and 75:
In Vitro Adulteration“Add after u
Page 76 and 77:
Urine SubstitutionOntario Smith
Page 78 and 79:
CholinesterasesButrylcholinesterase
Page 80 and 81:
Anion Gap ReliabilityLow Anion Gap
Page 82 and 83:
Forensics• Aid medical / legal in
Page 84 and 85:
Medical Review Officer• Licensed
Page 86 and 87:
Post MortemToxicology
Page 88 and 89:
Specimens• Blood• Reported as
Page 90 and 91:
Other sources
Page 92 and 93:
Legal Ethanol
Page 94 and 95:
The Barman’s Paradox• Legally d
Page 96 and 97:
The Clues1. Wide turns2. Straddling
Page 98 and 99:
What’s in a Drink?• 10 oz beer
Page 100 and 101:
• Specific gravity 0.8 g/ml• Vd
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“Breathanol”• [expired air] s
Page 104:
Photo References1. http://www.offth
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