Conformation Sensitive Capillary Electrophoresis Conformation ...

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Batch exampleFrag 1 Frag 2Plate 1FFrag 5Frag 1Frag 3Frag 3 Frag 4Pool 1Plate 2VPool 2Frag 5 Frag 6Plate 3NFrag 4Frag 2 Frag 6• All PCRs to work at one annealing temperature (61ºC)• Any fragment can be labeled in any colour

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Page 1: Conformation Sensitive Capillary El
Page 6 and 7: Universally tagged PCRM13F = tgt aa
Page 8 and 9: Assay Design: Standardised primerop
Page 10 and 11: Assay Design: Standardised primerop
Page 12 and 13: Primer optimisationAim:clean trace
Page 14 and 15: US1FBRCA2 Ex23GS1GS2[GS2] fmol/µl
Page 16 and 17: Optimisation•Experience gained ov
Page 18 and 19: Post PCR processing• Dilution ~1:
Page 20 and 21: Special cases Compound heterozyg
Page 22 and 23: Sequential loading
Page 24 and 25: Loss of resolutionPrimary injection
Page 26 and 27: Validation•Scoring system for CSC
Page 28 and 29: Conclusions• We have developed an

Conformation Sensitive Capillary Electrophoresis Conformation ...

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