PEG 400 by UHPLC with corona ultra

The Corona ® ultraCharged Aerosol DetectorResponse (peak area)3000002500002000001500001000005000001.11 min 1.34 min 1.56 min 1.76 min 1.94 min 2.09 min 2.24 min 2.37 min 2.49 min 2.60 minR 2 = 0.9945R 2 = 0.9925R 2 = 0.9944R 2 = 0.9949R 2 = 0.9965R 2 = 0.9965R 2 = 0.9992R 2 = 0.9991R 2 = 0.9998R 2 = 0.9990 500 1000 1500 2000 2500Total Mass on Column (ng)Figure 4. Response Curves for 18 ng to 2 μg Total Mass (oncolumn).ConclusionSensitive methods for the analysis of PEG400 can be obtained by either HPLC orUHPLC with Charged Aerosol Detection.The transfer of the method from HPLC toUHPLC offered a four-fold decrease inanalysis time and solvent consumption.The ability to detect low nanogramquantities of PEG 400 with limits ofdetection