PEG 400 at Low Levels by HPLC-CAD
PEG 400 at Low Levels by HPLC-CAD
PEG 400 at Low Levels by HPLC-CAD
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Corona TM Charged Aerosol Detector<strong>PEG</strong> <strong>400</strong> <strong>at</strong> low levelsApplic<strong>at</strong>ion noteThe need for universal <strong>HPLC</strong> detection inanalytical labor<strong>at</strong>ories is widespread.While several detection technologies(e.g., low wavelength UV, refractive index,evapor<strong>at</strong>ive light sc<strong>at</strong>tering, chemiluminescentnitrogen detectors) are currentlybeing used, there is significant room forimprovement in performance characteristicssuch as sensitivity, dynamic range,consistency of response factors andgradient or solvent comp<strong>at</strong>ibility.Response1.00.90.80.70.60.50.40.31.00.90.80.70.60.50.40.3Volts0.20.2To help address the many challenges ofuniversal detection, ESA has developedthe Corona <strong>CAD</strong> TM detector. This noveltechnology offers many benefits toanalytical scientists including:• High Sensitivity - <strong>Low</strong> ng limits ofdetection.• Consistent Response Factors - Responsemagnitude does not significantly dependon analyte properties (e.g. molarabsorbtivity, proton affinity).• Broad and Useful Dynamic Range - 4orders of magnitude (ng - µg quantities).• Excellent Reproducibility - Typically lessthan 2% RSD.• Broad Applicability - Can be used witha wide variety of <strong>HPLC</strong> conditions tomeasure virtually any nonvol<strong>at</strong>ile analyteincluding proteins, lipids, carbohydr<strong>at</strong>esand small molecules.• Ease of Use - Easy setup. Uses minimalbench space and requires only gas inputpressure and signal output range to beset.0.10.0-0.1-0.10 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15MinutesFigure 1. <strong>PEG</strong> <strong>400</strong> stock diluted 1:1000 (v/v) in mobile phase.(Gain range = 50pA full scale).This applic<strong>at</strong>ion note describes the use of the Corona <strong>CAD</strong> forthe measurement of polyethylene glycol (<strong>PEG</strong>) <strong>400</strong> (Figure 1).The method has excellent sensitivity (Figure 2) and a dynamicrange th<strong>at</strong> covers ng to µg levels. This is an example ofdetection of analytes th<strong>at</strong> possess only a weak chromophore.Corona parametersGas: 35psi via nitrogen gener<strong>at</strong>orFilter: noneRange: variable<strong>HPLC</strong> Parameters:Mobile Phase: 65:35; w<strong>at</strong>er:methanolFlow R<strong>at</strong>e: 0.5mL/minColumn: MD150, 3.2 x 150mm; 3µmColumn Temper<strong>at</strong>ure: ambientInjection Volume: 10µLSample prepar<strong>at</strong>ionStock solution (Sigma - P3265) was diluted in mobile phase.0.10.0
The CoronaTMCharged Aerosol Detector1.01.0ConclusionsResponse0.90.80.70.60.50.40.90.80.70.60.50.4The Corona <strong>CAD</strong> provides universal detection of nonvol<strong>at</strong>ileanalytes with response independent ofchemical properties, a wide dynamic response range,high sensitivity and good precision. Thesecharacteristics, along with reliability and simpleoper<strong>at</strong>ion, make this a superior detector for a widerange of <strong>HPLC</strong> analyses.0.30.30.20.10.0-0.1-0.10 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15MinutesFigure 2. <strong>PEG</strong> <strong>400</strong> stock diluted 1:100,000 (v/v) inmobile phase. (Gain range = 1pA full scale).0.20.10.0For more inform<strong>at</strong>ion about this applic<strong>at</strong>ion, theCorona <strong>CAD</strong>, or charged aerosol detection visitwww.coronacad.com. We are interested in your opinionsand are available to answer any questions youmay have: please contact a technical represent<strong>at</strong>ive <strong>at</strong>978.250.7082, or if e-mail is more convenient, sendyour questions to coronacad@esainc.com.Ordering inform<strong>at</strong>ionDescriptionCoronaThermal Organizer ModuleNitrogen gener<strong>at</strong>or 70-6003Pump, model 582 70-4050Autosampler, model 542 70-4152Qu<strong>at</strong>ernary low pressure gradientand degasser 70-5260Elite software including PC 70-5073Column, MD150 70-0636Part Number70-6350 (100/120V)70-6351 (230/240V)70-5499TAESA, Inc. • 22 Alpha RoadChelmsford, MA 01824-4171U.S.A.Tel: (978) 250-7000Fax: (978) 250-7090www.esainc.comwww.coronacad.comESA Analytical • Brook FarmDorton, AylesburyBuckinghamshireHP18 9NHEnglandTel: 44 (0)1844 239381www.esainc.comESA, Inc. is an ISO 9001 and 13485 Certified CompanyCorona TM and <strong>CAD</strong> TM are trademarks of ESA, Inc.ESA ®is a registered trademark of ESA, Inc.The Corona <strong>CAD</strong> is covered <strong>by</strong> multiple p<strong>at</strong>ents70-6521IA-1