Simultaneous Analysis of Glycerides and Free Fatty Acids in Palm Oil

Corona ® Charged Aerosol DetectorApplication noteTime %A %B Flow Rate0.00 100 0 1.55.00 100 0 1.511.00 96 4 1.524.00 37 63 1.535.00 32 68 1.545.00 18 82 1.958.00 15 85 1.960.00 5 95 1.563.00 100 0 1.565.00 100 0 1.5Table 1. Gradient Conditions.Results and DiscussionReproducibility of the method was tested by comparingfour different injections of the palm oil sample at 74µgcolumn load. The peak areas for an individual peak aswell as groups of peaks were observed and the resultsare shown in Table 2. The peak retention times exhibitedless than 1% variation over the gradient run for allsamples observed. Four overlays of the palm oil areshown in Figure 1. The chromatograms for the fourstandards run are illustrated in Figures 2A and 2B. Thecorrelation is done using a polynomial fit and was greaterthen >0.999 for all points (Figure 3). The LOD of the was