THE OSTIA VENAE HEPATICAE AND THE RETHROHEPATIC ...
THE OSTIA VENAE HEPATICAE AND THE RETHROHEPATIC ...
THE OSTIA VENAE HEPATICAE AND THE RETHROHEPATIC ...
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264 Faculty of Medicine Siriraj Hospital<br />
UTILITY OF GELATIN PARTICLE INDIRECT AGGLUTINATION TEST<br />
FOR SERODIAGNOSIS OF TOXOPLASMA GONDII IGG ANTIBODIES<br />
DETECTION OF TOXOPLASMA GONDII IGG ANTIBODIES<br />
Vanna Mahakittikun 1 , Sirichit Wongkamchai 1 , Sumalee Pogprasart 2 , Virach Junnu 1 , Dorn<br />
Watthanakulpanich 3<br />
1 Department of Parasitology, 2 Department of Clinical Pathology, Faculty of Medicine Siriraj<br />
Hospital, 3 Department of helminthology, Faculty of Tropical Medicine, Mahidol University,<br />
Bangkok 10700, Thailand.<br />
Key words : gelatin particle indirect agglutination test, GPAT, toxoplasmosis<br />
The antibody response against Toxoplasma gondii was evaluated by gelatin particle<br />
indirect agglutination test (GPAT) in 296 sera. The test was compared with p-30 ELISA for the ability to<br />
detect Toxoplasma specific IgG antibodies. Gelatin particle, an antigen carrier was sensitized with soluble<br />
antigen from sonicated Toxoplasma tachyzoites. The cutoff value of > 1:16 was considered positive. In<br />
296 sera studied, 286 sera (96.62%) gave the same qualitative result in both tests while the remaining 10<br />
sera (3.37%) show discrepancies results. Thirty-three of 37 (89.18%) sera from patients with symptoms<br />
suggestive of toxoplasmosis tested positive by GPAT and ELISA. By Kappa analysis, the correlation<br />
between antibody titer obtained with GPAT and ELISA was statistically significant (k = 0.849, p < 0.001<br />
and r 2 = 0.7068). The GPAT can be easily and rapidly performed without specialized equipment and<br />
likewise seems to be useful for the test of small number of specimens as well as mass screening or where<br />
laboratory facilities are limited.<br />
J Protozool Res 1999; 9(3) (in press)<br />
IMMUNOBLOTTING <strong>AND</strong> ENZYME LINKED-IMMUNOSORBENT<br />
ASSAY FOR DIAGNOSIS OF TOXOPLASMA INFECTION IN HIV<br />
THAI PATIENTS<br />
Sirichit Wongkamchai 1 , Vanna Mahakittikun 1 , Paron Dekumyoy 2 , Jerawan Onrotchanakun 1 .<br />
1 Department of Parasitology, Faculty of Medicine Siriraj Hospital, 2 Department of Parasitology,<br />
Faculty of Tropical Medicine, Mahidol University.<br />
Key words : immunoblot, enzyme-linked immunosorbent assay, Toxoplasmosis and HIV<br />
Toxoplasma gondii has been recognized as an important cause of morbidity and mortality<br />
among immunocompromised persons. The diagnosis of T. gondi infection is most often based on serological<br />
tests results. Serological diagnosis can be limited in AIDS patients because of depressed antibody<br />
responses. Fifty serum samples were used in this study to investigate serological evidence of toxoplasmosis<br />
in HIV positive Thai patients by Platelia kit, the commercial enzyme-linked immunosorbent assay<br />
(356)<br />
(357)
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