Burt, Dave - The Roslin Institute - University of Edinburgh
Burt, Dave - The Roslin Institute - University of Edinburgh
Burt, Dave - The Roslin Institute - University of Edinburgh
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Sequencing By Synthesis3’ 5’Cycle 1: Add sequencing reagentsFirst base incorporatedRemove unincorporated basesATCAGTCTGCTACGADetect signalDeblock and defluorPPP Base FluorGTCAGTACCCGATCGACycle 2-n: Add sequencing reagents and repeat• All four labeled nucleotides in one reaction• High accuracy• Base-by-base sequencingT5’16