Burt, Dave - The Roslin Institute - University of Edinburgh

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Infectious Bursal Disease• Also known as Gumboro disease• Caused by a Birnavirus (ds RNA)• Usually diagnosed at 3-6 weeks old• Spread through contaminated feed and water• Infects B-cells• Mortality can be up to 90% (usually around 20%)• Symptoms: anorexia, depression, diahorrea, ruffledfeathers, bursal lesions, immuno-suppression• Vaccination program (but different serotypes)48
Experimental Design• 3 spleen samples from control birds (lineBrL)• 3 spleen samples from IBDV-infected birds(4dpi) (line BrL)• Compared Affymetrix whole genomeexpression arrays with RNA-Seq49
Page 1 and 2: Next Generation SequencingCurrent S
Page 3 and 4: First Generation SequencingFrederic
Page 5 and 6: Genetic Maps• Genetic markers (e.
Page 7 and 8: QTL Mapping• QTL not mapped preci
Page 9 and 10: Genomic Tools• Expressed sequence
Page 11 and 12: Genomic Tools• Expressed sequence
Page 13 and 14: Sequencing TechnologiesNGS technolo
Page 15 and 16: Clonal Single Molecule ArraysAttach
Page 17 and 18: Base Calling from Raw DataT G C T A
Page 19 and 20: Applications19
Page 21 and 22: Avian GenomesFlightSmall genomeUniq
Page 23 and 24: Computational Pipeline forEnsembl/C
Page 25 and 26: Changes in gene family size along e
Page 27 and 28: Compare Rates of EvolutionSelection
Page 29 and 30: Rates Mammals > Birdsmovement of B
Page 31 and 32: Applications31
Page 33 and 34: Genome Wide Selection• Genotype 1
Page 35 and 36: % of SNPsNumber of SNPs/cMNumber of
Page 37 and 38: Annotation of SNPs%5045403530252015
Page 39 and 40: Final Panel Selection• 600K panel
Page 41 and 42: Applications of SNP Panel• Genomi
Page 43 and 44: AcknowledgementsFundingBBSRC/Defra
Page 45 and 46: RNA-Seq45
Page 47: Gene Models: TEF47
Page 51 and 52: Differential Gene ExpressionGene Sy
Page 53 and 54: Enrichment Analysis: MicroarrayMicr
Page 55 and 56: Advantages of RNA-Seq55
Page 57 and 58: AcknowledgementsFundingBiotechnolog
Page 59: Applications59
Page 62 and 63: MeDIP-Seq: NPAS462
Page 64 and 65: Sequencing TechnologiesNGS technolo
Page 66: Sequencing TechnologiesNGS technolo

Burt, Dave - The Roslin Institute - University of Edinburgh

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