Burt, Dave - The Roslin Institute - University of Edinburgh

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SNP Genotyping Panels• 3K• 6K• 20K• 42K• 60K• 600K192 samples/run125 million genotypes/run38
Final Panel Selection• 600K panel to be selected based on– Call rate <strong>of</strong> markers– Mendelian inheritance (MI)– Minimum allele frequency (MAF)– Linkage disequilibrium (LD)– Prediction <strong>of</strong> SNP effects on coding sequence39
Page 1 and 2: Next Generation SequencingCurrent S
Page 3 and 4: First Generation SequencingFrederic
Page 5 and 6: Genetic Maps• Genetic markers (e.
Page 7 and 8: QTL Mapping• QTL not mapped preci
Page 9 and 10: Genomic Tools• Expressed sequence
Page 11 and 12: Genomic Tools• Expressed sequence
Page 13 and 14: Sequencing TechnologiesNGS technolo
Page 15 and 16: Clonal Single Molecule ArraysAttach
Page 17 and 18: Base Calling from Raw DataT G C T A
Page 19 and 20: Applications19
Page 21 and 22: Avian GenomesFlightSmall genomeUniq
Page 23 and 24: Computational Pipeline forEnsembl/C
Page 25 and 26: Changes in gene family size along e
Page 27 and 28: Compare Rates of EvolutionSelection
Page 29 and 30: Rates Mammals > Birdsmovement of B
Page 31 and 32: Applications31
Page 33 and 34: Genome Wide Selection• Genotype 1
Page 35 and 36: % of SNPsNumber of SNPs/cMNumber of
Page 37: Annotation of SNPs%5045403530252015
Page 41 and 42: Applications of SNP Panel• Genomi
Page 43 and 44: AcknowledgementsFundingBBSRC/Defra
Page 45 and 46: RNA-Seq45
Page 47 and 48: Gene Models: TEF47
Page 49 and 50: Experimental Design• 3 spleen sam
Page 51 and 52: Differential Gene ExpressionGene Sy
Page 53 and 54: Enrichment Analysis: MicroarrayMicr
Page 55 and 56: Advantages of RNA-Seq55
Page 57 and 58: AcknowledgementsFundingBiotechnolog
Page 59: Applications59
Page 62 and 63: MeDIP-Seq: NPAS462
Page 64 and 65: Sequencing TechnologiesNGS technolo
Page 66: Sequencing TechnologiesNGS technolo

Burt, Dave - The Roslin Institute - University of Edinburgh

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