Curriculum vitae - Universität Heidelberg

CURRICULUM VITAE
Dr. Vytaute Starkuviene-Erfle
VIROQUANT/ BIOQUANT
Ruprecht-Karls Universität Heidelberg
Im Neuenheimer Feld 267
Heidelberg 69120
Tel.: 06221-5451259
Fax.: 06221-5451483
Email: vytaute.starkuviene@bioquant.uni-heidelberg.de
Personal details:
Date and place of birth: 6 th March 1970 in Lithuania
Nationality: Lithuanian
Family status: married
Languages: Lithuanian, English, German, Russian
Educational background:
1988-1992 Studies of Biochemistry, University of Vilnius, Lithuania (very good)
1992-1993 Diploma work at the Biotechnology Institute "Fermentas". Project:
"Purification and characterisation of a novel D-hydantoinase from
Bacillus circulans" (very good).

Chronology of employment
2007 September-to date
Junior Group Leader of “Screening of Cellular Networks” at the
VIROQUANT/BIOQUANT, Ruprecht-Karls University of Heidelberg.
2007 January-August
Project officer in the "Cell Biology and Biophysics Unit", in the
laboratory of Dr. R. Pepperkok, European Molecular Biology
Laboratory (EMBL), Heidelberg, Germany. FP6-EU funded project:
Novel post-genomic cell-based screens for drug targeting in membrane
protein disorders.
2001-2006
Postdoctoral fellow in the "Cell Biology and Biophysics Unit", in the
laboratory of Dr. R. Pepperkok, European Molecular Biology
Laboratory (EMBL), Heidelberg, Germany. NGFN funded projects: 1)
Establishment of an automated microscopy-based functional analysis
platform for the human proteome; 2) Novel molecular mechanisms
acting in secretory membrane trafficking.
1998-2001 Doctoral work at the Institute of Microbiology and Genetics, Georg-
August University Göttingen under the supervision of Prof. Dr. H-J.
Fritz. Project: Molecular mechanisms of DNA repair at high
temperatures: Identification and characterisation of thermostable uracil
glycosylases from archaeon Methanobacterium thermoautotrophicum
and bacterium Thermus thermophilus (Magna Cum Laude).
1997-1998 Visiting scientist in the "Biochemical Instrumentation Program", in the
laboratory of Prof. W. Ansorge, European Molecular Biology
Laboratory (EMBL), Heidelberg, Germany. Project: Construction of
novel recombinant thermostable DNA polymerases for PCR and
automated DNA sequencing”.

1995-1997 Research assistant in the Human Genetics Centre, Vilnius, Lithuania.
Project: Establishment of phenotype – genotype relationships in
patients affected by retinitis pigmentosa.
1993-1995 Research assistant in "Protein Structure and Interaction Laboratory",
Biotechnology Institute “Fermentas”, Vilnius, Lithuania. Project:
Optimisation of the synthesis of the optically active amino acids by
enzymatic catalysators.
Professional affiliations
2000 – Deutsche Gessellschaft für DNA-Reparaturforschung
2003 – European Life Scientist Organisation
2005 – Deutsche Gessellschaft für Zellbiologie

LIST OF PUBLICATIONS
Starkuviene V. and Pepperkok R. The Potential of High-content High-throughput
Microscopy in Drug Discovery. Review 2007 British Journal of Pharmacology,
September 152, 62-71.
Starkuviene V. and Pepperkok R. Differential requirements of the ts-O45-G and
procollagen biosynthetic transport. 2007 Traffic, August 8, 1035-1051.
Starkuviene V, Pepperkok R and Erfle H. Transfected cell arrays – an efficient tool
for high-throughput functional analysis. 2007 Expert review of proteomics, August 4,
479-489.
Li X, Kaloyanova D, van Eijk M, Eerland R, van der Goor G, Klumpermann J,
Lottspeich F, Starkuviene V, Wieland F and Helms B. Involvement of a Golgi-
resident GPI-anchored Protein in Maintenance of the Golgi Structure. 2007 Molecular
Biology of the Cell, April 18, 1261-1271.
Starkuviene V, Seitz A, Erfle H and Pepperkok R. Measuring secretory membrane
traffic: a quantitative fluorescence microscopy approach. 2007 Methods of Molecular
Biology, in press.
Bouchet-Marquis Cedric, Starkuviene V and Grabenbauer M. Golgi apparatus
studied in vitreous sections. 2007 J Microscopy, in press.
Starkuviene V*, Liebel U*, Simpson JC, Erfle H, Poustka A, Wiemann S, Pepperkok
R. High content screening microscopy identifies novel proteins with a putative role in
secretory membrane traffic. 2004 Genome Research. October 14: 1948-1956.
Henne A, Bruggemann H, Raasch C, Wiezer A, Hartsch T, Liesegang H, Johann A,
Lienard T, Gohl O, Martinez-Arias R, Jacobi C, Starkuviene V, Schlenczeck S,
Dencker S, Huber R, Klenk HP, Kramer W, Merkl R, Gottschalk G, Fritz HJ. 2004
The genome sequence of the extreme thermophile Thermus thermophilus.
Nature Biotechnology. May 22: 547-553.

Liebel U*, Starkuviene V*, Erfle H, Simpson JC, Poustka A, Wiemann S, Pepperkok
R. 2003 A microscope-based screening platform for large-scale functional protein
analysis in intact cells. FEBS Letters. Nov 20: 394-398.
Starkuviene V and Fritz HJ. (2002) A novel type of uracil-DNA glycosylase
mediating repair of hydrolytic DNA damage in the extremely thermophilic
eubacterium Thermus thermophilus. Nucleic Acids Research. 15: 2097-2102.
Luksa V, Starkuviene V, Starkuviene B, Dagys R. (1997) Purification and
characterization of the D-hydantoinase from Bacillus circulans. Applied Biochemistry
and Biotechnology. 62: 219-231.
* both authors contributed equally to this work.

INVITED TALKS
2007 April European Cystic Fibrosis Society Conference: “Large Scale Functional
Screens to Study Secretory Protein Traffic”.
2006 April European Cystic Fibrosis Society and Physiological Society Joint
Conference: Functional Genomics: “RNAi and cDNA over-expression
screens for cystic fibrosis”.
2006 March 29 th Annual Meeting of the German Society for Cell Biology:
“Differential regulation of ts-O45-G and PC I biosynthetic transport”.
2003 September ELSO 2003: “Functional screening assays to identify new proteins
involved in the secretory membrane traffic”.