i. Brain research2.27. To investigate how <strong>the</strong> cells in <strong>the</strong> brain process information, researchers surgically implanted electrodesinto <strong>the</strong> brains of two macaque monkeys. Researchers required <strong>the</strong> monkeys to sit still for hours in restraintchairs where <strong>the</strong>ir heads were fixed into place. Their brain activity and eye movements were monitored as <strong>the</strong>ywere made to stare at colour pictures of animals, nature scenes, patterns, and everyday objects presented on acomputer screen. They were given juice-drop rewards throughout <strong>the</strong> procedure in order to keep <strong>the</strong>m working.They had been deliberately deprived of water to make <strong>the</strong>m thirsty prior to each experimental session (Oram,2011). University of St Andrews, Scotland, UK.2.28. To find out whe<strong>the</strong>r childhood stress leads to an increased risk of developing psychiatric disorders inadulthood, researchers subjected baby rats to a sequence of stressful tests. On <strong>the</strong> first day (when <strong>the</strong> rats weretwenty-five days old), <strong>the</strong> rats were forced to swim in a water-filled tank from which <strong>the</strong>y could not escape forten minutes. The next day, <strong>the</strong>y were pushed into narrow plastic tubes to restrain <strong>the</strong>m for three periods of thirtyminutes each. On <strong>the</strong> final day, <strong>the</strong>y were placed in chambers where <strong>the</strong>y were given electric foot shocks everythirty seconds for three minutes. When <strong>the</strong> rats became adults, <strong>the</strong>y were subjected to various behavioural testsand assessed for signs of anxiety (Brydges et al., 2012). University of Edinburgh, Scotland, UK.j. Medical research2.29. So that researchers could investigate changes in heart wall stress and stretching associated with heartdisease, mix breed dogs were anaes<strong>the</strong>tised before undergoing open-chest surgery where part of <strong>the</strong>ir heartwas stretched by 22 per cent for six hours by a stretching device that was sewed directly onto <strong>the</strong>ir heart muscle.The dogs in one group were injected with a blood pressure–lowering drug before <strong>the</strong> stretch and again threehours after <strong>the</strong> stretch. The dogs were <strong>the</strong>n killed by electrical stimulation of <strong>the</strong> heart before <strong>the</strong>ir hearts weredissected. The experiment was funded by <strong>the</strong> British Heart Foundation and <strong>the</strong> NIHR Biomedical Research Centrein <strong>the</strong> UK but was probably conducted in <strong>the</strong> US (Hussain et al., 2010).2.30. In a study supported by Breast Cancer Research Scotland, breast tissue excised from twenty human patientswith breast cancer was surgically implanted under <strong>the</strong> skin of mice (six to twelve mice per patient). Four daysafter surgery, <strong>the</strong> mice were placed in chambers where <strong>the</strong>y were subjected to four hours of radiation, so thatresearchers could study <strong>the</strong> radiation’s effect on cancerous breast tissue. The mice were <strong>the</strong>n killed four hoursafter radiation exposure so that <strong>the</strong> xenografted breast tissue could be harvested (Coates et al., 2010). Universityof Dundee, Scotland, UK.k. Drug testing2.31. To test a potential drug treatment for lazy eye in humans, researchers anaes<strong>the</strong>tised fourteen kittens (twentyto twenty-six days old) in order to sew one of <strong>the</strong>ir eyes shut. The kittens were left in that state for two monthsbefore <strong>the</strong>y were subjected to brain surgery at <strong>the</strong> age of three months. The kittens were anaes<strong>the</strong>tised, andeach kitten’s eye was reopened. Both eyes were covered with a contact lens to help <strong>the</strong> eyes focus on a computerscreen placed fifty centimetres away from <strong>the</strong> kittens’ faces. The kittens were injected with a neuromuscularblocking agent (hereafter ‘NBA’) so that <strong>the</strong>ir eyes would not move. NBAs pose additional risks to animals because<strong>the</strong>y cause paralysis and prevent animals from indicating to researchers by movement or vocalisation that <strong>the</strong>yare actually coming round from <strong>the</strong> anaes<strong>the</strong>tic. The kittens’ scalps were cut to expose <strong>the</strong>ir skulls so that a pieceof skull could be removed to expose <strong>the</strong>ir brains. The researchers <strong>the</strong>n cemented a metal recording device into<strong>the</strong>ir skulls and covered it with a glass ‘window’ so that <strong>the</strong>y could see <strong>the</strong> kittens’ brains. A head-restrainingdevice was also glued onto <strong>the</strong>ir skulls to keep <strong>the</strong> head still during recording. Images were <strong>the</strong>n presented on<strong>the</strong> screens to stimulate <strong>the</strong> eyes while recordings were made for two hours. The test substance was also injectedinto <strong>the</strong>ir brains throughout <strong>the</strong> recording session. The kittens were woken up and <strong>the</strong>n subjected to fur<strong>the</strong>rrecording sessions one and two weeks later. At <strong>the</strong> end of <strong>the</strong> final session, all of <strong>the</strong> kittens were killed, and <strong>the</strong>irbrains were dissected (Vorobyov et al., 2013). University of Cardiff, Wales, UK.2.32. Eleven sheep had tubes surgically implanted into <strong>the</strong>ir spines for <strong>the</strong> administration of a new drug that wasthought to reduce pain sensitivity. Fourteen days after surgery, <strong>the</strong>ir legs were injected with formalin, which slowlydamaged <strong>the</strong> tissues, causing serious pain to <strong>the</strong> animals. Researchers recorded how often <strong>the</strong> sheep flinched12 <strong>Normalising</strong> <strong>the</strong> <strong>Unthinkable</strong>: The Ethics of Using Animals in Research
in pain or how long <strong>the</strong>y held <strong>the</strong>ir legs up because <strong>the</strong>y were unable to put any weight on <strong>the</strong>m. Blunt pins werealso pushed into <strong>the</strong>ir skin with increasing force until <strong>the</strong> sheep lifted up <strong>the</strong>ir legs in pain. Since <strong>the</strong> point of <strong>the</strong>experiment was to assess pain reactions, <strong>the</strong>re was no mention of pain relief during <strong>the</strong> tests, but <strong>the</strong>re was nomention of pain relief afterwards ei<strong>the</strong>r (Dolan et al., 2011). University of Glasgow, Scotland, UK.l. Eating disorders2.33. Researchers have used sheep as a model of human obesity. In one experiment <strong>the</strong>y kept eighteen young(ten-month-old) female sheep on <strong>the</strong>ir own in individual pens. Under surgery, tubes were implanted into <strong>the</strong>irheads so that drugs could be delivered directly into <strong>the</strong>ir brains. Over <strong>the</strong> next forty weeks, some of <strong>the</strong> sheepwere <strong>the</strong>n allowed to consume a high-calorie diet consisting of three times <strong>the</strong> normal amount of food required,while <strong>the</strong> o<strong>the</strong>rs were fed a restricted diet for forty weeks. Members of <strong>the</strong> ‘obese group’ were <strong>the</strong>n ‘put on a diet’and had <strong>the</strong>ir food restricted for sixteen weeks, while <strong>the</strong> o<strong>the</strong>rs were given high-calorie food to fatten <strong>the</strong>m upfor <strong>the</strong> remaining weeks. The sheep were subjected to regular anaes<strong>the</strong>sia so that <strong>the</strong>ir bodies could be scannedby a machine. They also had to undergo repeated blood-sample draws via cannulas in <strong>the</strong>ir jugulars as well asinsulin injections directly into <strong>the</strong>ir brains. At <strong>the</strong> end of this experiment, all of <strong>the</strong> sheep were killed, and <strong>the</strong>ir fatwas taken off <strong>the</strong>ir bodies and weighed (Adam et al., 2012). University of Aberdeen, Scotland.2.34. In an attempt to mimic anorexia, researchers fed mice severely restricted diets and encouraged <strong>the</strong>m toexercise, leading to severe weight loss. Mice were kept on an increasingly restricted feeding schedule for threemonths until <strong>the</strong>y were being given access to food for only two hours per day. Mice whose bodyweight droppedbelow 70 per cent of <strong>the</strong> normal bodyweight were killed. In one experiment, mice were kept in cages with runningwheels, where <strong>the</strong>y were encouraged to exercise excessively. In ano<strong>the</strong>r experiment, a chemical derived fromcannabis was injected into <strong>the</strong> animals daily to see whe<strong>the</strong>r it would increase <strong>the</strong>ir appetite. Some of <strong>the</strong> micehad to be killed due to <strong>the</strong> severe weight loss (Lewis and Brett, 2010). University of Strathclyde, Scotland, UK.2.35. Two conclusions can be drawn from <strong>the</strong>se examples and from <strong>the</strong> numbers and uses worldwide. First, <strong>the</strong>range of uses to which animals are subjected is very wide, and second, all biological categories of animals areutilised. Almost all of <strong>the</strong>se animals are sentient. Indeed, sentiency is a precondition of <strong>the</strong> need for a licence toexperiment under UK law and <strong>the</strong> current EU Directive. These sentient beings experience suffering, distress, harm,and death, as illustrated by <strong>the</strong> preceding examples.2.36. Since <strong>the</strong>se experiments cause not only physical and/or psychological harm but also death, it follows that<strong>the</strong>y require strong moral justification. We shall explore whe<strong>the</strong>r such justification is available to researchers, butfirst we shall look briefly at <strong>the</strong> history of <strong>the</strong> debate.3. The old debate3.1. Animal experimentation has been practised since <strong>the</strong> time of <strong>the</strong> Greeks, and possibly even before (Westacott,1949; Cohen and Loew, 1984). But it was only in <strong>the</strong> nineteenth century that experimentation began to be officiallyregulated through legislation. Since passage of <strong>the</strong> Cruelty to Animals Act 1876 in <strong>the</strong> UK, <strong>the</strong>re has been a long,and frequently acrimonious, debate between supporters and opponents of <strong>the</strong> practice. This old debate provideslessons for us and provides impetus for a new assessment. Numerous aspects of <strong>the</strong> old debate have effectivelyblocked progress in <strong>the</strong> discussion. Here we centre on four major aspects.Four blocks to ethical discussion3.2. The first block is nomenclature. The words ‘vivisection’ (meaning <strong>the</strong> practice of cutting an animal while alive)and ‘antivivisection’ (opposition to such a practice) became <strong>the</strong> standard terms of <strong>the</strong> pre- and post-1876 debate.In context, when <strong>the</strong> first uses of animals were preponderantly of this kind, <strong>the</strong> terms had some relevance.But <strong>the</strong> use of animals, as already shown, now widely exceeds such strictly invasive techniques. As such, soleconcentration on <strong>the</strong>se outdated terms now obscures <strong>the</strong> nature of <strong>the</strong> debate, which encompasses a myriad offactors, including <strong>the</strong> antecedent conditions of actual use (capture, rearing, transport, handling, and conditions ofA <strong>Report</strong> by <strong>the</strong> Working Group of <strong>the</strong> Oxford Centre for Animal Ethics13
- Page 4 and 5: 8. The failure of control 47Inspect
- Page 11: modified them to possess three muta
- Page 15 and 16: entirely absent from the notion of
- Page 19 and 20: esponses from animal models will be
- Page 21 and 22: 4.18. Genetically engineered ‘ani
- Page 23 and 24: 5. The changing ethical paradigm5.1
- Page 25 and 26: 5.15. It would be a mistake to mini
- Page 27 and 28: the moral realm to include obligati
- Page 29 and 30: 5.44. The fact that it is impossibl
- Page 31 and 32: 5.58. Briefly summarised, then, the
- Page 33 and 34: [intrinsically] wrong might neverth
- Page 36 and 37: 6.20. The second paragraph states t
- Page 38 and 39: The challenge, indeed the requireme
- Page 40 and 41: crisis situation in which one has t
- Page 42 and 43: as the ‘arbitrary favouring of on
- Page 44 and 45: 7.13. Thus, one primary obstacle fa
- Page 46 and 47: 7.26. Work on moral disengagement (
- Page 48 and 49: 8. The failure of control8.1. Despi
- Page 50: to-day care of the protected animal
- Page 53 and 54: 8.21. As one commentator made clear
- Page 55 and 56: 8.35. We shall address the issues o
- Page 57 and 58: 8.49. This disinclination to adopt
- Page 59 and 60: • There were appalling conditions
- Page 61 and 62: Harlan UK (1998-1999)9.13. In 1998-
- Page 63 and 64:
9.27. The BUAV called for an inquir
- Page 65 and 66:
• The Home Office report acknowle
- Page 67 and 68:
9.45. The ASC report concluded that
- Page 69 and 70:
10.8. Cannot animal research be jus
- Page 71 and 72:
11.2. This normalisation flies in t
- Page 73 and 74:
A Report by the Working Group of th
- Page 75 and 76:
Ascione, F. R. and Arkow, P. eds. 1
- Page 77 and 78:
H. Hammock, B. D. Beam, K. G. Chiam
- Page 79 and 80:
Garrett, J. R. 2012. The ethics of
- Page 81 and 82:
Available at: http://www.lifeissues
- Page 83 and 84:
Mitchell, L. R. 2012. Nonhumans and
- Page 85 and 86:
Russell, W. M. S. and Burch, R. L.
- Page 87:
A Report by the Working Group of th