Special Volume- BIOTECHNOLOGY - Facultatea de Biotehnologii

MINISTRY OF EDUCATION, RESEARCH AND INNOVATIONUNIVERSITY OF AGRONOMICAL SCIENCES ANDVETERINARY MEDICINE BUCHARESTPROGRAM ANDABSTRACTS2 nd International Symposium on New Researches inBiotechnology- Special Volume-BIOTECHNOLOGYISSN 1224-7774BUCHAREST2009

FOREWORDThe remarkable progress in biotechnology research would not be possiblewithout the communication and dissemination of the results first of all in theacademic environment.In this respect, the International Symposium organised in Bucharest by theFaculty of Biotechnology, at the University of Agronomical Sciences andVeterinary Medicine Bucharest, offered a great opportunity for discussing latestfindings on the topic to researchers, professors, lecturers, and biotechnologyspecialists from various institutions.Moreover, the symposium offered the opportunity to communicate anddiscuss current subjects in biotechnology, from different points of view and to formor consolidate partnerships for future technological R&D projects.The scientific manifestation drawn the participation of academics from theFaculty of Biotechnology and from other Romanian universities and researchinstitutes, together with well-known specialists from different countries, likeAustria, Bulgaria, France, Germany, Moldavia, Serbia.The symposium has been organised in five sections consisting of oralpresentations and posters, as follows: Section Agricultural Biotechnologies,Section Biotechnologies in Veterinary Medicine, Section Food Biotechnologies,Section Industrial and Environmental Biotechnologies, and Section Food Safety.The symposium proceedings are published in English, in the present volume.As I mentioned also in the foreword for the symposium, I would like to wishgood luck in their research activities in this wonderful and interesting field ofbiotechnology, optimism, health and personal achievements to all the participants.Furthermore, I wish for many more funding opportunities for the researchprojects in this field, for more and better project partnerships, and for moreattention to this field and to its researchers from the policy makers at national,European and international levels.Bucharest,November 2009Professor Petru NiculitaDean of the Faculty of Biotechnology3

ORGANIZERS:FACULTY OF BIOTECHNOLOGY& CENTER OF MICROBIAL BIOTECHNOLOGY BIOTEHGENScientific CommitteeHuub Lelieveld, GHI Association and EFFoST Executive CommitteeProf. Dr. Petru Niculita, Dean, Faculty of Biotechnology, USAMV BucharestProf. Dr. Peter Raspor, Biotechnical Faculty, University of LjubljanaProf. Dr. Cristina L.M. Silva, ISEKI Food, Catholic University of PortugalProf. Dr. Mona Elena Popa, Faculty of Biotechnology, USAMV BucharestOrganizing Committee - Faculty of Biotechnology, USAMV BucharestLecturer Dr. Florentina MateiProf. Dr. Mona Elena PopaLecturer Dr. Florentina Israel RomingLecturer Dr. Mioara VargaAssistant Dr Mihaela Ghidurus PanagopoulouAssistant Dr. Mira Turtoi4

2 nd International Symposium on “New Research in Biotechnology”19 -20 th November 2009, Bucharest, RomaniaPROGRAMNovember 19 th8.30 – 9.00 Registration9.00 – 9.20 Opening ceremony Prof. Dr. PETRU NICULITADean of the Faculty of Biotechnology,USAMV Bucharest, RomaniaSection I: Agricultural BiotechnologyChairman: Conf. Dr Ana ROSU, Faculty of Biotechnology, USAMV Bucharest, RomaniaVice-chairman: Lecturer Dr Silvana DANAILA, Faculty of Biotechnology, USAMV Bucharest,Romania9.20 – 9.40 EX SITU CONSERVATION THROUGHBIOTECHNOLOGICAL METHODSFOR ALL RARE AND ENDANGEREDDIANTHUS TAXA FROM ROMANIA9.40 – 10.00 THE INFLUENCE OF SAMPLESINCUBATION ON DETECTION OFPLRV AND THE EFFECT OFEXTRACTION BUFFER’S ADDITIVESON THE DETECTION OF POTATOVIRUSES Y, A , X AND S BY ELISATECHNIQUE10.00 – 10.20 COMPARATIVE STUDIES OFDYNAMICS AND STIMULATION OFALKALOID BIOSYNTHESIS INLEUCOJUM AESTIVUM L. CLONESIN VITROCRISTEA VICTORIA“Babes-Bolyai” University,“Alexandru Borza” Botanical Garden,Cluj-Napoca, RomaniaBADARAU CARMEN LILIANANational Institute of Research andDevelopment for Potato and SugarBeet Braşov, RomaniaSTANILOVA MARINAInstitute of Botany, BulgarianAcademy of Sciences, Bulgaria10.20 – 11.00 Coffee break & Poster viewing11.00 – 11.20 POTATO VIRUS Y (PVY)PURIFICATION AND ACHIEVEMENTOF ANTISERA FOR ELISAIDENTIFICATION OF INFECTEDPLANTS11.20 – 11.40 MORPHOLOGICAL ALTERATIONSIN TRANSGENIC PLANTS OFEUSTOMA GRANDIFLORUMOBTAINED BY TRANSFORMATIONWITH AGROBACTERIUMRHIZOGENESBADARAU CARMEN LILIANANational Institute of Research andDevelopment for Potato and SugarBeet Braşov, RomaniaPOPA GABRIELAUSAMV Bucharest,Faculty of BiotechnologyBucharest, Romania5

Section II: Biotechnology in Veterinary Medicine& Section IV: Industrial and Envinronmental BiotechnologyChairman: Prof. Dr. Stefana JURCOANE, Faculty of Biotechnology, USAMV Bucharest, RomaniaVice-chairman: Lecturer Dr. Florentina MATEI, Faculty of Biotechnology, USAMV Bucharest,Romania11.40 – 12.00 STUDIES ON DAIRY PRODUCTSENRICHMENT IN PHYTOSTEROLS12.00-12.20 METABOLIC PROFILES ANDHEALTH STATUS OF DAIRY COWSKEPT UNDER FREE AND TIE STALLSYSTEMS12.20 – 12.40 PHYTOSTEROL EMULSIONS FORFUNCTIONAL FOODS.PREPARATION AND STABILITY12.40 – 13.00 FOOD BIOTECHNOLOGY FOREDIBLE MUSHROOMS PRODUCINGBY USING A MODULAR ROBOTICSYSTEMPASCAL SVETLANA LIVIAFaculty of Food Science andEngineering, Department ofBiochemistry, Galati, RomaniaKIROVSKI DANIJELAFaculty of Veterinary Medicine,University of Belgrade, Belgrade,SerbiaPASCAL SVETLANA LIVIAFaculty of Food Science andEngineering, Department ofBiochemistry, Galati, RomaniaPETRE MARIANUniversity of Pitesti,Faculty of SciencesRomania13.00 – 14.30 LUNCH14.30 – 14.50 CONSERVATION OF WINE YEASTBIODIVERSITY IN THE CONTEXT OFAUTOCHTONOUS WINEPRODUCTION14.50 – 15.10 BIOTECHNOLOGY OF MEDICINALMUSHROOMS CULTIVATION BYSUBMERGED FERMENTATION OFCEREAL BY-PRODUCTSSection III: Food BiotechnologyMATEI FLORENTINAUSAMV Bucharest,Faculty of BiotechnologyBucharest, RomaniaPETRE MARIANUniversity of Pitesti,Faculty of SciencesRomaniaChairman: Conf. Dr Amalia MITELUT, Faculty of Biotechnology, USAMV Bucharest, RomaniaVice-chairman: Assistant Dr Mihaela GHIDURUS PANAGOPOULOU, Faculty of Biotechnology,USAMV Bucharest, Romania15.10 – 15.50 THE POWER OFMICROENCAPSULATION; ANOVERVIEW OF THE APPLICATIONSIN FOODS15.50 – 16.10 MICROSATELLITE MARKERS FORSACCHAROMYCES SP.CHARACTERIZATION: INTERESTFOR PHYLOGENY, BIOLOGY ANDTECHNOLOGY6BORDA DANIELAFaculty of Food Science andEngineeringUniversity Dunarea de Jos, Galati,RomaniaLEGRAS JEAN-LUCUMR 1131 – SVQV équipe MSV -INRA Université de Strasbourg,France

16.10 – 16.30 CONSUMER ATTITUDES TO NOVELFOOD TECHNOLOGIES IN EUROPE16.30 – 17.00 Coffee break17.00 – 18.30 Poster session19.30 – 21.30 DinnerNovember 20 thSection V: Food SafetyPOPA ALEXANDRAUSAMV Bucharest,Faculty of BiotechnologyBucharest, RomaniaChairman: Prof. Dr. Mona Elena POPA, Faculty of Biotechnology, USAMV Bucharest, RomaniaVice-chairman: Lecturer Dr. Florentina ISRAEL-ROMING, Faculty of Biotechnology, USAMVBucharest, Romania9.00 – 9.20 AN OVERVIEW OF ANALYZINGMETHODS USED INESTABLISHING FOODTRACEABILITY9.20 – 9.40 CASE STUDY REGARDING MILKQUALITY AND SAFETY9.40 – 10.00 APPLICATIONS OF WOODCOMPOSITE BIO-BASEDMATERIALS IN FOOD PACKAGINGSTERIAN ADRIANAUSAMV Bucharest,Faculty of BiotechnologyBucharest, RomaniaTURTOI MIRAUSAMV Bucharest,Faculty of BiotechnologyBucharest, RomaniaMOLDOVAN LAURENTIUSC RODAX IMPEX SRBucharest, Romania10.00 – 10.20 FLEXIBLE AUTOMATICEQUIPMENT FOR FOODPACKAGING ON PROCESSINGLINE FOR FOOD SAFETY GROWTHPANTEA GABRIELASC RODAX IMPEX SRBucharest, Romania10.20 – 11.00 Coffee break & Poster viewing11.00 – 11.10 COMPARATIVE STUDYREGARDING NITRATE RETENTIONIN SOME LEAFY AND ROOTYVEGETABLES11.10 – 11.30 EVIDENCE OF NATURALOCCURANCE OF MYCOTOXINS INCORN CULTIVATED IN ROMANIA11.30 – 11.50 RESEARCH FUNDINGOPPORTUNITIES11.50 – 12.10 Closing ceremony12.10 – 13.30 LunchPANAGOPOULOU GHIDURUSMIHAELA ELENAUSAMV Bucharest,Faculty of BiotechnologyBucharest, RomaniaISRAEL-ROMING FLORENTINAUSAMV Bucharest,Faculty of BiotechnologyBucharest, RomaniaPOPA MONA ELENAUSAMV Bucharest,Faculty of BiotechnologyBucharest, Romania7

2 nd International Symposium on New Research in BiotechnologyNovember 19-20 th 2009, Bucharest, RomaniaORAL PRESENTATIONS8

O I.1 EX SITU CONSERVATION THROUGHBIOTECHNOLOGICAL METHODS FOR ALL RARE ANDENDANGERED DIANTHUS TAXA FROM ROMANIAVICTORIA CRISTEA 1 , JARDA LILIANA 1 , IRINA HOLOBIUC 2 ,MAGDALENA PALADA 3 , ADELA HALMAGYI 41 Alexandru Borza Botanical Garden, Babes-Bolyai University, 42 Republicii str., 400015, Cluj-Napoca, Romania2 Inst. of Biology , 296 Independentei spl., 0600031, Bucharest, Romania3 Forest Research and Management Institute,1 Biscaia str, 335900, Simeria, Romania4 Biological Research Institute, 48 Republicii str., 400015, Cluj-Napoca, RomaniaEx situ conservation by the means of in vitro culture biotechnology was employed in thecase of all Rumanian endangered or endemic taxa belonging to Dianthus genus aiming to realize anational collection. Plant material was collected from the Natura 2000 sites or from other protectedareas. For each taxon we selected at least three distinct populations, each of one comprising three tofive individuals. The plant taxa we have studied are: D. callizonus, D. giganteus ssp. banaticus, D.glacialis ssp. gelidus, D. henteri, D. pratensis ssp. racovitzae, D. dobrogensis, D.spiculifolius, D.tenuifolius and D. nardiformis. We have successfully achieved the in vitro culture for all selectedtaxa, hence, in a first stage obtaining a multiplication rate of five to thirty five new plantlets/explant.In vitro micropropagated material was then crioconserved, and the regeneration was monitored.Genetic analyses using biochemical and molecular markers are in progress. If by these geneticanalyses somaclonal variations are not rendering evident, the in vitro micropropagated plantmaterial will be employed for repopulations in critical conditions. As a conclusion, we may afford tosustain the idea that in vitro cultures and a national collection currently represent the mainbiotechnology aiming for biodiversity conservation.Supported by 31-008/2007 PN2 project (Romanian Ministry of Education and Research)9

O.I.3 COMPARATIVE STUDIES OF DYNAMICS ANDSTIMULATION OF ALKALOID BIOSYNTHESIS IN LEUCOJUMAESTIVUM CLONES IN VITROM. STANILOVA 1∗ , E. MOLLE 2 , Y. BOGDANOVA 1 , B. PANDOVA 3 , S. YANEV 31 Institute of Botany, Bulgarian Academy of Sciences, Sofia, Bulgaria2 University of Forestry, Faculty of Ecology and Landscape Architecture, Sofia, Bulgaria3 Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia, BulgariaKey words: galanthamine, lycorine, genotype, tyrosine, phenylalanineShoot-clumps of twelve Leucojum aestivum in vitro clones of different origin and with specificalkaloid profiles were studied comparatively. The biosynthesis was stimulated by some alkaloidprecursors: casein hydrolysate, and/or amino acids - tyrosine and phenylalanine - added in the MSbased nutrient media supplemented with BAP and NAA. The effects of the treatments wereconcerned by alkaloid content and profile changes based on the previously observed dynamics.∗ Corresponding author: M. Stanilova, E-mail: maris@bio.bas.bgO.I.4 POTATO VIRUS Y PURIFICATION AND ACHIEVEMENT OFANTISERA FOR IDENTIFICATION OF INFECTED PLANTS BYELISA TECHNIQUE1 COJOCARU N., 1 CARMEN LILIANA BĂDĂRĂU, 2 MIHAELA DOLOIU1 National Institut of Research and Development for Potato and Sugar Beet Braşov2 Inspectoratul Teritorial pentru Certificarea Semintei si a Materialului Saditor BrasovKey words: antiserum, PVY, ELISAMultiplication of potato virus Y was made on tobacco plants. The leaves were harvested 3-4 weeksafter inoculation.In the event of potato virus Y, for homogenization of tobacco leaves, we used bufferwith phosphat-citrat (McIlvaine) 0,18 mol/l, pH 7, surplus DIECA and thioglycolat Na, and afterhomogenization was added Triton X-100. The virus purification was done through two differentialcentrifugation cycles followed by centrifugation on sucrose gradient (10-14%) and next on cessiumchloride. For rabbit immunization were gave 2 injections/rabbit with purified virus, surplus Freundadjuvant.Maximum concentration of immune antiserum was 1:8192 for Y antisera. From respectiveantisera, through purification was separated fraction G of immunoglobulin (IgG) and to realized theconjugate, antibodies were marked with alkaline phosphatase. Optimum dilution for IgG andconjugate also was 1:1500.11

O.II.1 METABOLIC PROFILES AND HEALTH STATUS OF DAIRYCOWS KEPT UNDER FREE AND TIE STALL SYSTEMSDANIJELA KIROVSKI*, IVAN VUJANAC*, HOREA ŠAMANC*, NATALIJAFRATRIĆ*, DRAGAN GVOZDIĆ*, ŽELJKO SLADOJEVIĆ # , SLAVČA HRISTOV a2*Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia#Veterinary station „Veterina system Sladojević”, Gradište, Bosnia and Herzegovina, Republic of SrpskaªFaculty of Agriculture, University of Belgrade, Belgrade, SerbiaCorresponding author: dani@vet.bg.ac.rsKey words: dairy cows, metabolic status, free and tie stall systemsThe aim of this study was to examine the difference in metabolic profiles and health statusin dairy cows kept under free and tie stall systems. A total of ten cows from farm A (free stall system)and ten cows from farm B (tie stall system) were chosen for this study. Blood samples were taken 15days before, as well as 15, 60 and 150 after parturition. Body condition scoring was determinateduring dry period, puerperium, early and late lactation period. Health status was observedcontinuously during peripartal period (3 weeks before to 3 weeks after calving). The followingparameters were measured in blood serum: total protein, albumin, urea, glucose, BHBA, totalbilirubin and total calcium. Our results related to metabolic profiles indicated that cows on farm Bhad significantly higher total protein and albumin concentrations in all examined periods, whichindicate on severe dehydration in cows, kept under tie stall system. Additionally, there weresignificantly more cases of pododermatitis, mastitis, distocia and retention secundina in dairy cowson farm B. In conclusion, cows kept under tie stall system are in greater risk of diseases then cows infree stall system.13

O.III.1 THE POWER OF MICROENCAPSULATION: ANOVERVIEW OF THE APPLICATIONS IN FOODSFaculty of Food Science and Engineering, University Dunarea de Jos, GalatiBORDA DANIELAMicroencapsulation is the science of entrapping bioactive compounds referred to as the encapsulantor the core and delivering them into small particles called microcapsules. Encapsulation holds ahuge technological potential and it already has a high number of applications within the foods, butalso pharmaceuticals, chemical, cosmetic and printed industries.In food products fats, oils, aroma compound and oleoresins, vitamins, minerals, colorants, enzymes,bacteria,nutraceutical and antimicrobial substances have been microencapsulated.Understanding the complexity of microencapsulation and the diversity of applications requires agood knowledge of the mechanisms by which compounds are encapsulated and released but also thephysico – chemical properties of the compounds.There are greater demands being made on theintegrity of the microcapsules to provide controlled delivery of the core material at a specificsite/target and at a desired time.A review of the current methods and applications in biotechnology is necessary to foresee the furtherdirections of microencapsulation as a way to add value and improve the quality of existing foodproducts.O.III.2 MICROSATELLITE MARKERS FOR SACCHAROMYCES SP.CHARACTERIZATION: INTEREST FOR PHYLOGENY,BIOLOGY AND TECHNOLOGYUMR 1131 – SVQV équipe MSV - INRA Université de Strasbourg, FranceLEGRAS JL, MASNEUF-POMAREDE IRecently, microsatellite typing has been applied successfully to the characterization of S.cerevisiaestrains. This technique offers an unprecedented discrimination method and revealed unexpectedphylogenic features but also enabled us to explain some technological properties. However, therecent detection of other Saccharomyces or interspecific (S. cerevisiae*S. kudriavzevii or S.cerevisiae*S. uvarum) hybrids implies the need for complementary techniques. In order to fill thisgap, we have developed two sets of microsatellite markers for S. uvarum or S. kudriavzevii. Thesemarkers enable us to offer a powerful characterization at strains level of the parental species andtherefore achieve a complete characterization of their interspecific hybrids. These techniques permitalso to compare the phylogeny obtained from strains of the three species and to compare somebiological features for each species. This approach is an essential step towards the reconstruction oftailored hybrid strains with desired properties.14

O.III.3 CONSUMER ATTITUDES TO NOVEL FOODTECHNOLOGIES IN EUROPEUniversity of Agronomic Sciences and Veterinary Medicine Bucharest, RomaniaCorresponding author: alexandrapopa1@gmail.comALEXANDRA POPAKey words: consumer acceptance, novel food technologies, risk communicationIn recent years, several food technologies emerged giving the opportunity for new productdevelopment. These novel food products faced different levels of consumer acceptance, especially inEurope. Because of several food scares in the past two decades, European consumers became morecautious about their food choices, in terms of food safety and health. Thus, launching novel foodswith genetically modified organisms was a failure on this market because of negative consumeracceptance. In the same time, functional foods – like the probiotic yoghurt – were successful. Thecase of GM food and of the other novel foods is discussed in this paper. It is concluded that riskcommunication and sound food policies- like educational programmes are crucial for the futuresuccess of emerging food technologies, like nutrigenomics, food irradiation or nanotechnology.O.IV.1 STUDIES ON DAIRY PRODUCTS ENRICHMENT INPHYTOSTEROLSLIVIA S. PASCAL, RODICA SEGAL∗ Dept. of Biochemistry, SIA Galati, Romania, e-mail: livia.pascal@yahoo.comKey words: phytosterol, functional foods, yogurt, viscosity, syneresis.Phytosterols help in reducing the blood level of the cholesterol contained in LDL due to their similarstructure with cholesterol, and thereby the consumption of functional foods with phytosterols can bean important factor in the reduction of risk for cardiovascular diseases. Phytosterols enjoy the statusof GRAS compounds (Generally Recogniyed As Safe) recognized by the American Authority Food andDrug Administration (FDA). Functional dairy products with phytosterols are currently available inmore than 20 countries, including 13 EU countries. Since increased interest is focused on theincorporation of phytosterols in dairy-based products, the objective of the present study were toinvestigate the incorporation of phytosterol emulsions obtained from sunflower oil deodorizerdistillate, into milk and yogurt. To achieve this purpose, milk with different fat content (0.1%, 1.5%and 3.5%) were analyzed for its behavior. The dairy products were evaluated in terms of acidity, pH,viscosity, taste, odour, color and the syneresis phenomen.15

O.IV.2 PHYTOSTEROL EMULSIONS FOR FUNCTIONAL FOODS.PREPARATION AND STABILITYLIVIA S. PASCAL ∗ , RODICA SEGAL ∗ , STEFAN DIMA ∗∗ , OANA CONSTANTIN ∗∗∗Key words: phytosterol emulsion, ultrasound method, crystallization, emulsion stabilityRecently, more and more researches highlight the role of phytosterols on decreasing total serumcholesterol and LDL cholesterol. In accordance with Food and Drug Administration, a dalily intakeof 1,3 g of phytosterols induce a reduction of cholesterol up to 15%. Because phytosterols areinsoluble in water and slightly soluble in oils, their biodisponibility can be increased only byinclusion in the oil phase of an oil/water emulsion, which can represent a premix for functional foods.In our researches it was established the optimum conditions for the stability of emulsions and aninclusion up to 35% of phytosterols. Emulsions were made by ultrasound method, with differentvegetable oils as organic phase saturated in phytosterols, Tween 20 as emulsifiant (HLB=16.7) andas crystallization inhibitors were used lecithin and tristearin. The stability of the emulsions wasevaluated by using arabic gum and sodium caseinate, in aqueous solution of 2%, at different pHvalues (5.7 and 4.6). The microscope analysis showed that most phytosterol quantity is solubilized orin microcrystal shape in the oil phase of the prepared emulsions. The prepared emulsions were usedas premixes for dairy functional foods.∗ Dept. of Biochemistry, SIA Galati, Romania, e-mail: livia.pascal@yahoo.com∗∗ Dept. of Colloid chemistry, Faculty of Sciences, Galati, Romania∗∗∗ Dept. of Biotechnology, SIA Galati, Romania16

O.IV.3 FOOD BIOTECHNOLOGY FOR EDIBLE MUSHROOMSPRODUCING BY USING A MODULAR ROBOTIC SYSTEMMARIAN PETRE 1 , ALEXANDRU TEODORESCU 1 , ADRIAN NICOLESCU 2 ,MARCEL DOBRE 2 , DANIELA GIOSANU 11 University of Pitesti, Faculty of Sciences2 “Politehnica” University of Bucharest, Faculty of Applied SciencesKey words: food biotechnology, edible mushrooms, modular robotic system, winery wastes, vinewastesThe main reasons to valorise all the specific wastes of vineyard ecosystems and wine producingindustry were focused on setting up a food biotechnological procedure to be applied by using amodular robotic system in order to get protein food in the shape of fruit bodies and composts to beused as protein feed. The experiments were carried out by screening the optimal biotechnology ofrecycling the winery and vine wastes by using them as growing substrata for edible and medicinalmushrooms and protecting in this way the natural environment. In this respect, the new biotechnologyof solid state cultivation of edible and medicinal mushrooms Lentinula edodes and Pleurotusostreatus was applied by using a modular robotic system that provides the following fully automaticoperations: sterilization of composts, inoculation in aseptic chamber by controlled injection devicecontaining liquid mycelia as inoculum, incubation as well as mushroom fruit bodies formation inspecial growing chambers with controlled atmosphere and the picking up of edible and medicinalmushroom fruit bodies. All these operations must be carried out in a completely aseptic zone in whichno human operator has any access during the whole mushroom cultivation in continuous flow up tothe culture cycle end. By applying these fully automatic procedures, all physical and chemical factorsthat could influence the fungal biomass production as well as fruit bodies formation of L. edodes andP. ostreatus mushroom species could be strictly lead and controlled in order to get ecological foodproducts.17

O.IV.4 CONSERVATION OF WINE YEAST BIODIVERSITY INTHE CONTEXT OF AUTOCHTHONOUS WINE PRODUCTIONFLORENTINA MATEI 1 , AUREL CIUBUCA 2 , ELENA BRINDUSE 31 USAMV Bucharest, Faculty of Biotechnology, Romania2 SCDVV Bujoru, Galati, Romania3 ICDVV Valea Calugareasca, Prahova, RomaniaKey words: wine yeast, biodiversity, isolation, conservation, autochthonous productsBiodiversity is one of a main subject on European level researches of the useful microflora inagricultural and food industry. Although these researching themes which are approaching thissubject have a profound regional character that is watching the typicality of some products (Cognacand Champagne-France, , France, Spain, Hungary), it can observed a tendency to integrate researchat European level for some biodiversity elements (for example the spreading of yeast along theDanube or inside the Balcanic space).Previous works proved that local wine yeast perform better under winemaking conditions on localgrape varieties than the commercial strains. The aim is to obtain local wines with local strains inorder to keep the authenticity of the products and finally to be part of the market.More than 400 yeast strains have been isolated in the past two years from consecrated vineyards(Dealu Mare-Valea Calugareasca, dealurile Bujorului andIasi from Moldova county, Minis Maderat-Est of Romania). All these strains have been identified by rapid physiological tests API 20CBiomerieux, and 70 of them have been kept as Saccharomyces species to be tested under winemakingconditions.This work belongs to the project PNCDI II 51-065 DIVINMOL supported by the Romanian Ministryof Education, Research and Innovation.18

O.V.1 AN OVERVIEW OF ANALYZING METHODS USED INESTABLISHING FOOD TRACEABILITYADRIANA STERIAN, MIHAELA GEICU, MONA POPA *Keywords: traceability, food safety, nuclear magnetic resonance, isotope ratio mass spectrometry,near infrared reflectance spectroscopyTo give consumers a guarantee on animal nutrition it requires the existence of reliable methods forthe characterization of meat and milk.For the analysis of meat, milk and animal tissues origin, potential markers for feed can be used(biomarkers of plants, markers of geographic origin of milk and meat). Diet and animal habitatsinfluences the isotopic composition of water and fat entering the composition in animal products ortissue. The animal nutrition determines the composition of fat and in particular the ratio ofunsaturated and saturated fatty acids. Using nuclear magnetic resonance (NMR) to identify the typeof milk in nutrition, the relative ratio of saturated, unsaturated and polyunsaturated fatty acids canbe determinate. Another method of determining the type of food and geographical origin is the IRMS(isotope ratio mass spectrometry).Traces of stable isotopes in animal tissues or products reflects thecomposition of stable isotopes of the feed, modified by the animal's metabolism. It has been shownrecently that using NIRS (near infrared reflectance spectroscopy) to spectrally characterize meat,the difference between beef samples originating from two types of feeding system can be made (feedbased only on grazing versus feeding based on silos of corn).As traceability refers to all processes down to origins, it is necessary to obtain information onanimal identification, geographic and native origin, which are often important considerations in thequality labeling and in food adulteration prevention.20

O.V.2 CASE STUDY REGARDING MILK QUALITY AND SAFETYMIRA TURTOI, MIHAELA GHIDURUS, PETRU NICULITA, MONA POPAEnsuring an acceptable level of food quality and safety is necessary to protect consumersand facilitate trade. These objectives can be achieved by implementing and monitoring qualityassurance throughout the total food chain when necessary and possible. Anyone involved in the foodchain, from farmer to consumer, has the responsibility to maintain safe food taking necessaryprecautions so that they are protected from hazards responsible of food borne diseases. These actionswill prevent and reduce food losses important especially for areas where food security is threatened.The research presented in this paper is aiming to establish the way ecological farms affectthe quality and safety of cow's milk. Having set this target samples of cow's milk from organic farmswere examined compared with those derived from conventional farms from the physico-chemical andmicrobiological point of view: pH, titrated acidity, content of dry soluble substance, total aerobicplate count and the number of E. coli/coliforms.The results showed that from the physico-chemical point of view, the indicators of milksamples from the ecological farms registered increased values when compared with milk samplesfrom the conventional farm, while from the microbiological point of view, the microbial load of milksamples from the ecological farms was lower when compared with milk samples from theconventional farm.O.V.3 APPLICATIONS OF WOOD COMPOSITE BIO-BASEDMATERIALS IN FOOD PACKAGING* SC RODAX IMPEX SRL Bucharest, RomaniaL. MOLDOVAN*, G. PANTEA*, M. BECEA*, D. GHEORGHIU*Key words: food packaging, bio-based materials, biodegradable materialsIn the last decades, food packaging research efforts were mainly devoted to barrier materials (newpolymers, complex and multilayer materials). Changes in retailing practices such as globalisedmarkets resulting in longer distribution distances present major challenges to the food packagingindustry and act as driving forces for the development of new and improved packaging concepts thatextend shelf life while maintaining and monitoring food safety and quality. Simultaneously and apartfrom initiative to reduce the weight of conventional materials, development of new sustainable,recyclable and/or biodegradable packaging materials also contributes to increasing the overallpresence of eco-friendly packaging solutions across the food chain. Forest resources and compositematerials will be part of this dynamic future. A variety of bio-polymers have been extracted frombiomass, synthesized from bio-derived monomers, or produced from microorganisms. Bio-basedplastic applications are currently targeted towards single-use, disposable, short-life packagingmaterials, coatings for paper, etc. Products of renewable resources having low cost and suitableproperties for packaging applications are going to make them the most preferable material in thenear future.21

O.V.4 FLEXIBLE AUTOMATIC EQUIPMENT FOR FOODPACKAGING ON PROCESSING LINE FOR FOOD SAFETYGROWTHL. MOLDOVAN*, D.CIOBOATA*, D. GHEORGHIU*, G. PANTEA*, N.HOISESCU** SC RODAX IMPEX SRL Bucharest, RomaniaKeywords: food safety, packaging method, packaging equipment, vacuum packagingSafe foods represents a consumer right who has to be conscious that his health and his own safe isprotected. The development and the diversification of the products and of the g 3 oods consumption,necessitate the development and the improvement of the activities in the field of techniques and of theeconomy of the packaging. In the last decade the most important additional function of the packagingmethod is the extension of the shelf life of the food products .Usually the food products are packed inplastic bags. The plastic bag which prior to packaging has been vacuumated, ensures that the packedproduct will have a pleasant appearance and its shelf life will be longer. By thermo-skrinking thepackage, takes the product shape. This packaging line is designed to provide great flexibility (to beeasy to adjust in accordance with the production demands) and to be properly placed in productionlines. Both, vacuum-packaging equipment and the thermo-shrinking by dipping equipment, have asimple construction and a semiautomatic working cycle.O.V.5 COMPARATIVE STUDY REGARDING NITRATERETENTION IS SOME LEAFY AND ROOTY VEGETABLESMIHAELA GHIDURUS, AMALIA MITELUT, MIRA TURTOI,PETRU NICULITA, MONA POPAUniversity of Agronomy and Veterinary Medicine Bucharest -Faculty of BiotechnologyKey words: leafy, rooty, vegetables, nitrate, nitrate, fertilizationThe aim of the study was to determine the potential activity of nitrate accumulation in someleafy and rooty vegetables grown in open field conditions by means of an enzymatic method. In thisregard the following vegetables species were grown: spinach, lettuce, carrots and onions, which werefertilized in the field with 5 doses of fertilizer resulting in the same number of experimental variantsfor each species.The nitrate and nitrite content of the analyzed vegetables varied considerably. The highestvalue of nitrate was retained by the leafy vegetables especially lettuce, however the results obtainedshowed that the majority of analyzed lettuce samples had nitrate values placed within the EUlegislation for Summer Lettuce (3500 mg/kg fresh), implying that the fertilization dose of 100 kg Nper hectare in open field may be the maximum treatment quantity.22

O.V.6 EVIDENCE OF NATURAL OCCURRANCE OFMYCOTOXINS IN CORN CULTIVATED IN ROMANIAISRAEL-ROMING FLORENTINA, BOICU SIMONA, LUTA GABRIELA, BALANDANIELA, GHERGHINA EVELINA, DECIU GEORGIANA, SIMION VASILICAUniversity of Agronomical Sciences and Veterinary Medicine Bucharest, Faculty of Biotechnology / Centre forApplied Biochemistry and Biotechnology BIOTEHNOLKey words: mycotoxins, ochratoxin A, deoxynivalenol, maizeA total of 125 maize samples were analyzed for determination of deoxynivalenl and ochratoxin A 25maize hybrids were cultivated in five regions of Romania using the same agronomic practices. Bothdeterminations were made by HPLC methods with immunoaffinity clean up. The limit of detection(LOD) was 20 µg/kg for deoxynivalenol and 30 ng/kg for ochratoxin A. Deoxynivalenol results werepositive for 31 samples and ochratoxin A results were positive for 29 samples. Only one sampleexceeded deoxynivalenol limit for food, but not for feed. Regarding ochratoxin A , there are 4 sampleswith levels higher than 5 ppb, making them improper for human consumption.23

2 nd International Symposium on New Research in BiotechnologyNovember 19 – 20 th 2009, Bucharest, RomaniaPOSTER PRESENTATIONS24

SECTION I: AGRICULTURAL BIOTECHNOLOGYI.1 THE REACTIVITY OF FRAGARIA X ANANASSA DUCH. REGENERANTS TOWARDS THE INVITRO TREATMENT WITH FUNGIC ELICITORSCOGĂLNICEANU GINA, BREZEANU AURELIA, MITOI MONICA,HELEPCIUC FLORENTA, MATEI MIRELA, MATEI SORINI.2 RESEARCH ON CORRELATIONS BETWEEN THE QUALITY PARAMETERS OF SOMEVARIETIES OF WHITE WINES OBTAINED AT THE OSTROV VINEYARD CENTERCULEA RODICA- ELENA, TAMBA-BEREHOIU RADIANA,POPA NICOLAE-CIPRIAN, POPESCU STELAI.3 MICROMULTIPLICATION RESEARCH REGARDING POTATO PRODUCTION FROM FOURROMANIAN VARIETIES SUITABLE FOR SUSTAINABLE AGRICULTURE (ASTRAL,CHRISTIAN,ROCLAS AND MAGIC)S. DANAILA-GUIDEA,P. NICULITA,N. CHIRU,A. ROSUI.4 THE HYDROMINERAL TREATMENT FOR PLANTS FOR ACHIEVING NUTRITIONALQUALITY AND HIGHER PRODUCTIONEVELINA GHERGHINA, GABRIELA LUTA, FLORENTINA ISRAEL – ROMING,DANA BALANI.5 SOMATIC EMBRYOGENESIS INDUCTION IN PRESENCE OF MODERATE OSMOTICSTRESS, SYNTHETIC SEEDS PRODUCTION IN RARE DIANTHUS SPECIES FROM ROMANIANFLORA AS TOOL FOR EX SITU CONSERVATIONHOLOBIUC I., BREZEANU AURELIA, BLINDU R.I.6 IMPACT OF CRYOPRESERVATION ON BIOCHEMICAL PARAMETERS OF IN VITROCULTURED HYPERICUM RUMELIACUM BOISS.K.DANOVA,M.URBANOVA,M.SKYBA,E.CHELAROVA,M.STEFANOVA,D.KOLEVA,V.KAPCHINA-TOTEVAI.7 MICROPROPAGATION AND BIOLOGICAL ACTIVITY OF LEONURUS CARDIACA L.M. DIMITROVA, M.HRISTOVA, D. DRAGOLOVA, V. KAPCHINA-TOTEVAI.8 INFLUENCE OF HYDRIC STRESS ON SOME VALUABLE BIOCOMPOUNDS IN CEREALSGRAINSGABRIELA LUTA, EVELINA GHERGHINA, FLORENTINA ISRAEL-ROMING,DANIELA BALAN, GEORGE MUSATI.9 INVESTIGATIONS OF CHAENOMELES JAPONICA (THUNB.) LINDL. IN BULGARIATEODORA MIHOVA-CHAVDAROVA, PENKA MONDESHKA,VIOLETA KONDAKOVAI.10 PRODUCTION OF POTATO MINITUBERS THROUGH HYDROPONIC TECHNIQUEANDREEA NISTOR, NICOLETA CHIRU, DIANA KARACSONYI,GHEORGHE CAMPEANU, NICOLAE EUGEN ATANASIUI.11 THE ANALYSE OF DIFFERENT POTATO VARIETIES ON PRE-BASIC SEED PRODUCTIONPROCESS, IN GREENHOUSE, USSING MINITUBERSANDREEA NISTOR, NICOLETA CHIRU, DIANA KARACSONYI,GHEORGHE CAMPEANU, NICOLAE EUGEN ATANASIUI.12 PRELIMINARY RESULTS CONCERNING THE GRAPEVINE BACTERIAL CANCERAURELIA PODOSU, MARIA PAMFIL, GH. MIHU, ILEANA STOIAN,LACRAMIORA MIRON,I.13 MARIOARA BOSOI, IONICA BOSOI INFLUENCE OF SOIL MOISTURE AND SEEDTREATMENT ON GERMINATION AND INITIAL DEVELOPMENT OF MANGOLDSVILEN RAYKOVI.14 SPATIALITY AND DURABILITY OF TILLAGE INFLUENCE ON THE BULK DENSITY OFLEACHED SMOLNITSAIVAN DIMITROV, SVILEN RAYKOV25

I.15 PTR-MS DETECTION OF NEPETALACTONE IN SHOOT CULTURES OF THREE NEPETASPECIES GROWN UNDER DIFFERENT CARBOHYDRATE SOURCEJ. NESTOROVIĆ, D. MIŠIĆ, B. ŠILER, S. ŽIVKOVIĆ, A. STOJIĆ,M. PERIŠIĆ. D. GRUBIŠIĆI.16 INFLUENCE OF SOME ABIOTIC FACTORS ON PLEUROTUS SPP. MYCELIAL GROWTHZAGREAN VALENTIN, CAMPEANU GHEORGHE, NEGULESCU PETRE,ATANASIU NICOLAEI.17 IN VITRO PROPAGATION OF BULGARIAN LINE ECHINACEA PURPUREA L.TS.DZANFEZOVA, A.DZURMANSKI, ZH.YORDANOVA, V.KAPCHINA-TOTEVAI.18. INFLUENCE OF CRYOPRESERVATION ON THE BASIC PHYSIOLOGICAL INDECES INPROPAGATED IN VITRO ORTHOSIPHON STAMINEUS BENTHZH.YORDANOVA, M.URBANOVA, E.CHELAROVA, TS.GANEVA.,D.KOLEVA, V.KAPCHINA-TOTEVAI.19 THE EFFECT OF NACL AND SUGARS ON SECOIRIDOID GLUCOSIDES PRODUCTION INROOT CULTURES OF TWO CENTAURIUM SPECIES (FAM. GENTIANACEAE)D. MIŠIĆ, B. ŠILER, J. NESTOROVIĆ, D. GRUBIŠIĆSECTION III: FOOD BIOTECHNOLOGYIII.1 MODIFFICATIONS OF THE TARTARIC COMPOUNDS SOLUBILITY DURINGMALOLACTIC FERMENTATION OF SOME RED AND WHITE WINESODĂGERIU G., BĂLĂNUŢĂ A., NEACŞU I., ZAMFIR I. C., COTEA V.V.,RUSU E.,VACARCIUC L.,MORARU IIII.2 STUDY OF COLOR EVOLUTION DURING SHELF LIFE OF APPLE PUREE TREATEDWITH PULSED ELECTRIC FIELDMIHAELA GEICU, MONA POPA, AMALIA MITELUT, PETRU NICULITA,RADU CRAMARIUCIII.3 THE CHARACTERIZATION OF THE CU, ZN, CO, CD AND FE CONTENT OFLACTOBACILLUS AND BIFIDOBACTERIUM STRAINS USED FOR PROBIOTIC PRODUCTIONANA DESPINA IONESCU, CASARICA ANGELA, ELENA BOCA,RAMONA IONELA COJOCARU, RUSU NICOLETA, DOBRE NICOLETA,IONICA ILEANA ADRIAN VAMANU, EMANUEL VAMANUIII.4 TECHNIQUES OF INCREASING MARKETING PERFORMANCE BY OPTIMIZING THESUPPLY CHAIN STRUCTUREMARGARIT GABRIELA,VISAN LUMINITA, EPURE ALEXANDRUIII.5 EIGHT CELL DISRUPTION METHODS FOR THE OBTAINING OF SPENT BREWER ’ SYEAST CELL WALLMARINESCU GINA, STOICESCU ANTONETAIII.6 CORRELATION OF ANTIOXIDANT PROPERTIES OF SOME VEGETAL EXTRACTSOBTAINED FROM HYPERICUM PERFORATUM L., EVALUATED BY CHEMILUMINESCENCEAND LIPID PEROXIDATION ASSAYCORNELIA NICHITA, GEORGETA NEAGU, VIRGINIA VULTURESCU,RADU ALBULESCU, NICOLETA BADEA, IONICA ILEANAIII.7 ANTIOXIDATIVE CHARACTERISTICS OF CALENDULA OFFICINALIS L. EXTRACTSDETERMINATED BY CHEMILUMINESCENCE TEHNIQUECORNELIA NICHITA, GEORGETA NEAGU, VIRGINIA VULTURESCU, RADU ALBULESCU,MARIA GIURGINCA, DOBRE NICOLETAIII.8 PRELIMINARY CHARACTERISTICS OF MYOFIBRILLAR PROTEINS OBTAINED FOREDIBLE BYOFILMS REALISATIONFLORICEL CERCEL, LIVIA PATRAŞCU, PETRU ALEXE, MARIANA STROIU26

III.9 VALORIZATION OF BIODEGRADABLE MATERIALS USING BIOTECHNOLOGYMONA POPAIII.10 SCREENING OF RHODOTORULA STRAINS IN ORDER TO SELECT A GOOD PRODUCERFOR CAROTENOID PIGMENTSGEORGETA RADULESCU, AURORA SALAGEANU, AURELIA CHIRVASE ,EUGENIA MOCANU, IONELA COJOCARU, GABRIELA SAVOIU,FLORENTINA DAMSA, NICOLETA DOBRE, ILEANA IONICAIII.11 METHODS FOR OBTAINING ANTHOCYANINS FROM RASPBERRY PLANTSKAVITHA RAVICHANDRAN, NAY MIN MIN THAW SAW, HEIDI RIEDEL,CAI ZHEN ZHEN, IRYNA SMETANSKAIII.12 STRESS-INDUCED MODIFICATION OF THE LIPID MEMBRANE COMPOSITION OFOENOCOCUS OENI CELLS: CHARACTERIZATION OF THE O. OENI CFA GENE BYHETEROLOGOUS COMPLEMENTATION OF A LACTOCOCCUS LACTIS CREMORIS CFA-DEFICIENT STRAINTHI-MAI-HUONG TO, COSETTE GRANDVALET, RAPHAELLE TOURDOT MARECHALIII.13 IMPACT OF STRESS CONDITIONS ON THE GROWTH OF LACTOBACILLUSACIDOPHILUS IBB 801 AND PRODUCTION OF ACIDOPHILIN 801MEDANA ZAMFIR, SILVIA GROSU-TUDORIII.14 IMMUNOMODULATORY EFFECTS OF A SELECTIVE EXTRACT OBTAINED FROMCRATAEGUS MONOGYNA JACQVIRGINIA VULTURESCU, CORNELIA NICHITA, GEORGETA NEAGU, LUCIANALBULESCU, RADU ALBULESCU, MANUELA MIHALACHESECTION IV: INDUSTRIAL AND ENVIRONMENTAL BIOTECHNOLOGYIV. 1 STUDIES CONCERNING ENZYMATIC ACTIVITY OF ASPERGILLUS NIGER 401 ANDTRICHODERMA REESEI 4122 STRAINS USED IN HYDROLYSIS OF ENERGY CROPSANGHEL LAURA, PETRE STELIAN MATEI, ANAMARIA NICOLAE,CAMELIA DIGUTA, STEFANA JURCOANEIV.2 ISOLATION OF PHOTOTROPHIC AND HETEROTROPHIC BACTERIA WITH POTENTIALFOR GASOLINE CONSUMPTIONARDELEAN I.I, GHITA S., SARCHIZIAN I.IV.3 EPIFLUORESCENT METHOD FOR QUANTIFICATION OF PLANKTONIC MARINEPROKARYOTESARDELEAN I.I, GHITA S., SARCHIZIAN I.IV.4 RESEARCHES CONCERNING INHIBITION OF ACTIVITY OF SOME MATRIXMETALLOPROTEASESDANIELA BALAN, FLORENTINA ISRAEL-ROMING, GABRIELA LUTA,EVELINA GHERGHINAIV.5 IN VITRO PROPAGATION AND XANTHONE CONTENT OF GENTIANELA PRAECOX (A. ETJ. KERN) DOSTALT. BANJANAC, D. KRSTIĆ, M. DEVIĆ, B. VINTERHALTER, D. GRUBIŠIĆIV.6 ALTERATIONS IN ENZYME ACTIVITIES IN LEAVES AFTER EXPOSURE OF WHEATPLANTS TO 900 MHZ ELECTROMAGNETIC FIELDMILENA DIMITROVA, DANIELA DRAGOLOVA, MARGARITA KOUZMANOVAIV.7 DOES 900 MHZ ELECTROMAGNETIC FIELD INDUCE OXIDATIVE STRESS IN WHEATPLANTS?DANIELA DRAGOLOVA, MILENA DIMITROVA, MARGARITA KOUZMANOVA27

IV.8 ADAPTATIVE RESPONSE OF SHEWANELLA PUTREFACIENS AND PSEUDOMONASAERUGINOSA TO TOXIC ORGANIC SOLVENTSMM LAZAROAIEIV.9 BACTERIAL XYLANASE FROM VEGETABLE WASTE PRODUCTSMIHAELA CLAUDIA MARINESCU, GHEORGHE CAMPEANU,CORINA IONESCU, MISU MOSCOVICI, RADU ALBULESCU,ELEONORA GHEORGHIU, RAMONA MARDALE, IONICA ILEANAIV.10 COMPARISON BETWEEN CLASSICAL IDENTIFICATION METHODS FOR YEAST ANDRAPID PHYSIOLOGICAL TESTS API 20-CANA-MARIA NICOLAE, GETUTA NICOLAE, ELENA BRINDUSE, FLORENTINA MATEIIV.11 CONSERVATION METHODS USED FOR YEAST ISOLATED FROM VINEYARDS –LYOPHILISATION ADVANATGESGETUTA NICOLAE, ANA-MARIA NICOLAE, PETRE STELIAN,FLORENTINA MATEI, STEFANA JURCOANEIV.12 GROWTH OF PSEUDOMONAS AERUGINOSA IN PILOT BIOREACTOR AND ITS TESTINGIN THE BIOREMEDIATION OF SOILS TREATED WITH PESTICIDES SUCH ASDINITROPHENOLS.PETRE STELIAN MATEI, ANGHEL LAURA, GETUTA NICOLAE,CAMELIA DIGUTA, JURCOANE STEFANASECTION V: FOOD SAFETYV.1 ROMANIAN ECOLOGICAL FOOD MARKET: AN OVERVIEWMIHAELA DRĂGHICI, ALEXANDRA POPA, MONA POPA, PETRU NICULITA,GEICU MIHAELA, AMALIA MITELUTV.2 COLOUR CHANGES IN MINCED PORK MEAT INOCULATED WITH PSEUDOMONASAERUGINOSA AND TREATED BY OHMIC HEATINGAMALIA MITELUŢ, MIHAELA GEICU, MONA POPA,PETRU NICULITA, RADUCRAMARIUC,IOANA VĂTUIU, DANIELA VĂTUIU, MIRCEA POPESCUV.3 ELECTRONIC NOSE FOR DISCRIMINATION OF ROMANIAN APPLESELENA PRUTEANU, DENISA DUTA, FULVIA HINCU28SECTION VI: MISCELLANEOUSVI.1 IMPROVED PRODUCTION OF BACTERIAL CELLULOSE BY ACETOBACTER XYLINUMUSING DIFFERENT CARBON SOURCESANGELA CASARICA, CORINA IONESCU, GHEORGHIU ELEONORA, MARIANA SOARE,MISU MOSCOVICI, DOBRE ALINA, IONICA ILEANAVI.2 MODERN STUDIES FOR OBTAINING VOLATILES OILS FROM MEDICINAL ANDAROMATIC PLANTS IN ORDER TO REALIZE THE THERAPEUTIC BIOPREPARATESMARIA ICHIM, RAMONA ENACHE,IULIA ADRIANA GRAFU, ADRIANA VISAN, ALICE ARMATUVI.3 STUDIES CONCERNING THE PREPARATION AND THE CHARACTERIZATION OFNAFTIFINE LOADED LIPOSOMESCRISTINA HLEVCA, ELENA PATRUT,MINERVA PANTELI, RASHIT YUKSEL, RODICA GUTAVI.4 THE INFLUENCE OF EXTRACTIVE TECHNIQUES ON RADICAL SCAVENGING ACTIVITY

OF POLYGONUM AVICULARE L. EXTRACTSGEORGETA NEAGU, CORNELIA NICHITA,VIRGINIA VULTURESCU, GEORGETA RADULESCUVI.5 OLIGOPROANTHOCYANIDINS INHIBITS THE GENOTOXICITY OF CICLOPHOSPHAMIDEIN VIVOGEORGETA NEAGU, VIRGINIA VULTURESCU,CORNELIA NICHITA, BEATRICE BURGHELEA, RADU ALBULESCUVI.6 SUCCESSFULLY APPLICATION OF INNOVATION IN TRADITIONAL TECHNOLOGIESE. MARCU, G. NEDITAVI.7 KINETICAL CHARACTERIZATION OF LIPOSOMES WITH ANETHI AETHEROLEUMALINA ORTAN, CRISTINA DINU PIRVUVI.8 THE EFFECT OF FORMULATION PARAMETERS ON THE CHARACTERISTICS OF SOMETRANSFEROSOMES WITH STEROID HORMONESCRISTINA DINU PIRVU, CRISTINA HLEVCA,ALINA ORTAN, ELENA PATRUT, LUIGI SILVESTROVI.9 FEEDBACK MECHANISMS CONNECTED TO CLIMATE CHANGE DYNAMICSR. PASCUVI.10 THE INFLUENCE OF CHEMICAL AND ORGANIC FERTILIZATION ON WHEAT QUALITYMARIA M. SANDRIC, CLAUDIA E. MOSOIU, V. SIMACVI.11 BACTERIAL CELLULOSE MEMBRANES CONTAINING SILVER PARTICLES USED ASANTIBACTERIAL MATERIALSA. STOICA, L. M. DRAGNE, S. JINGA, M. STROESCU, I. JIPA, C. JINGAVI.12 ELECTRONOGRAPHIC HIGHLIGHTING OF THE LUMINISCENCE OF SOMESTRUCTURES FROM NATURERADIANA TAMBA-BEREHOIU, CIPRIAN POPA, STELA POPESCU, RODICA CULEAVI.13 THE DETERMINATION OF BEST MARKETING STRATEGY BY THE PRODUCERS BASEDON CONSUMERS’ BEHAVIOR DURING THE ECONOMIC CRISISMARGARIT GABRIELA, TOMA RADU CRISTIAN, STOENOIU CRISTINAVI.14 UTILISING THE MONOFACTORIAL VARIANCE ANALYSIS TO DETERMINE HOWTEMPERATURE IS INFLUENCING THE MYCELIA GROWTH RATEVARGA M., VIŞAN L.VI.15 LYSOZYM ENZYMATIC ACTIVITY CHANGES IN CONTROL OF MALOLACTICFERMENTATION USED IN ROMANIAN WINESLUMINIŢA VIŞAN, GABRIELA MĂRGĂRIT, DIANA GROPOŞILĂ, MIOARA VARGA29

30SECTION I: AGRICULTURAL BIOTECHNOLOGYP I.1 THE REACTIVITY OF FRAGARIA X ANANASSA DUCH.REGENERANTS TOWARDS THE IN VITRO TREATMENT WITHFUNGIC ELICITORSGINA COGĂLNICEANU*, AURELIA BREZEANU*, MONICA MITOI*,FLORENŢA HELEPCIUC*, MIRELA MATEI**, SORIN MATEI***Institute of Biology, Bucharest** Research Institute for Soil Science and Agrochemistry, BucharestObjective: the goal of the present study was to describe the ultrastructural, structural andbiochemical changes induced in regenerants of Fragaria X Ananassa Duch. by the in vitro treatmentwith fungic elicitorsMaterials and methods:• Biological material: We used regenerants from multiple axilary multiplication on growth media(Murashige-Skoog basal, with Gamborg B 5 vitamins, indolilbutiric acid (IBA) 0.1 mg/l, giberellicacid (GA 3 ) 0.1 mg/l, benzylaminopurina (BAP) 1 mg/l, sucrose 30 g/l and agar 7 g/). Thetemperature in the growth chamber was 24 o C±2 o C, while light (2400 lux) was provided 16hrs/day.• Experimental design: We used seven experimental variants, each with five replicates: M – control(no treatment), M-AD control (treatment with distilled water), E1 - treatment with anatoxinicserum obtained from Botrytis cinerea strains F1 (BcF1), E2 - treatment with anatoxinic serumobtained from microbial strains Bc F1, Bc F7, Bc S1, Bc P2, E3 - treatment with anatoxinicserum from Trichoderma viride strains P 456 (TvP456), TvP1, ThP8 and Penicilliumchrysogenum A2 (Pc A2) , E4 – treatment with anatoxinic serum obtained from strains Bc F1, BcF7, Bc S1, Bc P2, TvP456, TvP1, ThP8, Pc A2. For PD1-E4 and PD2-E1 the treatments weredone with variants of E4 and E1 serum which differed in concentration, temperature or time ofobtaining procedure.• Treatment: we repeated three treatments at 48 hours, with 1 ml extract each. The extract was appliedunder sterile conditions at the leaf level, with an insulin syringe. Sampled were taken 24 hrs afterthe last treatment.• Biological parameters analysed: Electron microscop observations were done on leaf pieces standardpreparated (Mascorro and Bozola, 2007). For light microscopy thick sections (1-2 µm) werestained with 1% toluidine blue in 1% borax (Pickett-Heaps, 1966). We studied antioxidantenzymes in the extract obtained from grinding the plantlets with quartz sand in phosphate buffer0.05 M, pH 7, with 2 mM EDTANa 2 and 4% (w/v) PVP, at 4 0 C. After centrifugation at 15000 rpmfor 15 min, superoxid dismutase (SOD), catalase (CAT) and guaiacolperoxidase (GPX) weremeasured in the supernatant. SOD was measured according to Beauchamp and Fridovich (1971),

CAT according to Beers and Sizer (1952), and GPX according to Chance and Maehly (1955).Total protein concentration was measured according to Bradford (1976).Results:Strawberry regenerats showed a complex response to the treatment with fungic elicitors thatfrequently reached foliar necrosis. Growth and differentiation were correlated with the differenttreatments. The foliar tissue contained both normal cells and stressed cells that occasionally reachednecrosis. The nucleus and nucleolus did not show major changes. The mitochondria had an electronopaquestroma and expanded crista. The plastidia presented major changes, especially at thelamellar level, were: i. tylacoids appear as tubules of varying size, either randomly or paralleldistributed, with an electron-dense content, and ii. disorganized tylacoids present large expansionsthat can form vesicules of the plastidial sacs, with transparent content. Sometimes, ultrastructuralorganization of the chloroplast was almost completely destroyed. Starch, plastoglobuls and plastidialribosoms are absent. Deposition of electron-dense material was observed at the tonoplast, plasmamembrane and cell wall level. Biochemical analysis revealed that the treatments with inactivatedtoxins induced a decrease in SOD activity in all experimental variants, except E1. CAT activityslightly decreased in all experimental variants except in PD2-E4 where is showed a slight increase.When compared with the total protein concentration, CAT activity showed a slight increase, exceptfor E1. Both SOD and CAT are key components in regulating Reactive Oxygen Species (ROS). Moststudies on oxidative stress induced by pathogens or treatments with different elicitors report anincrease in ROS concentration and of the activity of antioxidant enzymes. Several studies havereported a decrease in enzyme activity after pathogen attack (e.g. tomato plants infected with Botritiscinerea, (Malolopsza and Urbanek, 2000), or after treatment with substances that induce resistancelike salicylic acid, 2,6-dichloroizonicotinic acid or benzotriazol (Conrath et al., 1998). GuaiacolPOX activity decreased in all experimental variants except for PD2-E4. A similar response wasreported by Pacolla et al. (2008) in treatments with micotoxin T2 produced by strains from the genusFusarium in tomato plants. In our experiment we report an increase in the activity of some of theanionic isoperoxidases, especially for the variants E2 and E3, but not associated with an increase oftotal POX activity. Overall, electrophoretic analyses of POX and total citosolic proteins showedsignificant differences between experimental variants. The only uniform response to the stressinduced by the different treatments was protein synthesis, which decreased in all experimentalvariants except for E1, in which despite a decrease in the activity of antioxidant enzymes, proteicconcentration increasesThe financial support for this research was provided by the PNCDI-II Contract 31-078/2007.31

P.I.2 RESEARCH ON CORRELATIONS BETWEEN THE QUALITYPARAMETERS OF SOME VARIETIES OF WHITE WINESOBTAINED AT THE OSTROV VINEYARD CENTERCULEA RODICA- ELENA*, TAMBA-BEREHOIU RADIANA*,POPA NICOLAE-CIPRIAN**, POPESCU STELA** University of Agricultural Sciences and Veterinary Medicine Bucharest** S.C. FARINSAN S.A., GiurgiuKey words: white wines, quality parameters, correlation coefficients, regressions94 samples of white unbottled wine have been analyzed. The samples were derived fromthree varieties of grapes (White Feteasca, Blanc Sauvignon, Italian Riesling) grown in SC Ostrov S.A.The following quality parameters were determined for each sample: d 20 20,, alcohol (% vol), total dryextract (mg/l), free sugar (g/l), unreducing extract (g/l), total acidity (g/l C 4 H 6 O 6 ), free SO 2 (mg/l) andtotal SO 2 (mg/l). Statistical analysis showed that the best regressors for the studied white wines arethe parameters Free sugar and Total dry extract. These regressors are involved in 2 of the 3significant correlations between the quality parameters that are common to all analyzed varieties,namely: total dry extract - free sugar, total dry extract – unreducing extract and free sugar - alcoholcontent. For each of the three studied varieties we established a number of specific correlations.Thus, for the variety of White Feteasca wine, Total acidity was correlated with the Density parameter(r = 0.45 **), Total dry extract (r = 0.57 ***) and Dry unreducing extract (r = 0.49 **). For the wineof the Italian Riesling variety, Alcohol content was correlated with Total dry extract (r = 0.58 ***),with Unreducing extract (r = 0.47 **) and with Free sugar (r = 0.58 * **). The Blanc Sauvignonvariety was characterized by correlations of the indices related to wine sulphitation, with otherparameters. Thus, Free SO 2 correlated with the percentage of Alcohol (r = 0.38 *) and Total SO 2with the Total dry extract (r = 0.82 ***) and with the Unreducing extract (r = 0.53 **).32

P.I.3 MICROMULTIPLICATION RESEARCH REGARDINGPOTATO PRODUCTION FROM FOUR ROMANIAN VARIETIESSUITABLE FOR SUSTAINABLE AGRICULTURE (ASTRAL,CHRISTIAN, ROCLAS AND MAGIC)S. DANAILA-GUIDEA*, P.NICULITA*, N. CHIRU**, A. ROSU*, I.M. STANCIU*.* USAMV, Faculty of Biotechnology - Bucharest, Romania** INCDCSZ - Brasov, RomaniaKey words: potato cultivars, in vitro multiplication, sustainable agriculture.Producing healthy biological material represents a laborious activity because in eachcultivation year the tuber can be infected with viruses, mycoplasma and bacteria. Using microtubersproduced in vitro from varieties suitable for sustainable agriculture, can be obtained aphytosanitary healthy cloned material, a natural product, without chemical residues, a cleanfoodstuff able to satisfy increasing consumers and EU standards requirements(4,5,7).Our experiment was concentrated on elaborating a rapid and efficient micromultiplicationprotocol for potato obtained from 4 varieties suitable for sustainable agriculture(ASTRAL,CHRISTIAN, ROCLAS and MAGIC), using micro tubers obtained in vitro by an single nodexplant method. A total number of 7 experimental variants containing of phytohormons formulawere tested. It has been noticed that the variant number 7 (V7C= MS (1962)+2,25 mg /lBAP+ 0,18mg./l IAA, 8 gr./l agar+ 30 gr./l zaharoza) assured a high morphogenetic response.The utilization of these method will open new possibilities of understanding themechanisms by which artificial cultivation conditions will influence vegetal entities and represents atotally reproducible guide mark for all who are interested in obtaining planting material forpotatoes from varieties suitable for sustainable agriculture(8).33

P.I.4 THE HYDROMINERAL TREATMENT FOR PLANTSFOR ACHIEVING NUTRITIONAL QUALITYAND HIGHER PRODUCTIONEVELINA GHERGHINA*, GABRIELA LUTA*,FLORENTINA ISRAEL – ROMING*, DANA BALAN*Key words: mineral substances, biochemical constituents, hydro mineral treatments, superiornutritional qualities, higher production.Plants, the green gold of mankind, represent the main food source for both human and animal,being at the same time the row material in some branches of the light industry. Fallowing thisidea, plants must be studied and their value must be enhanced by complex means, depending onthe constituting biochemical compounds, on the nutritional and technological potential that isprovided.Accompanying the obtaining of a new plant form of higher quality (lines, hybrids, sorts) itis necessary to apply the latest biotechnologies that imply real progress so that a superiornutritional quality of the plant is permitted, with low energy consumption and a great economicefficiency.Each part of a vegetable mechanism has its own specific job that contributes tometabolically processes, yet ensuring the plants functionality as a great whole. In carrying outworldwide "the green revolution” it is necessary to know the biochemical mechanisms that lieat the basis of biological phenomena in vegetable organisms, with the aim of influencing anddirecting, according to plant’s nature and the aimed goal, the biochemical processes in orderto increase output of photosynthesis, enlargement of biomass, increasing the amount ofproteins, glucide vitamins and other active substances.The presence of water and mineral substances in all biological systems pleads for theindispensable character of these compounds in live organisms. Plants contribute in asignificant part in the circuit of mineral substances in nature. Thanks to the capacity of mineralsubstances to form numerous compounds with organic substances, they have contributed, in asubstantial way, to the enrichment and diversity of biochemical constituents in vegetableorganisms.These reasons being given, the research regarding hydro mineral treatments in guidingthe biocompounds accumulation metabolisms for plants with the aim of obtaining products androw materials in the food industry with superior nutritional qualities, represents research ofgreat present interest in what concerns the quality of the alimentation and also from theeconomic perspective.34

P.I.5 SOMATIC EMBRYOGENESIS INDUCTION INPRESENCE OF MODERATE OSMOTIC STRESS, SYNTHETICSEEDS PRODUCTION IN RARE DIANTHUS SPECIES FROMROMANIAN FLORA AS TOOL FOR EX SITU CONSERVATIONInstitute of Biology. Romanian Academy, BucharestE-mail:iriholo@ibiol.roHOLOBIUC I., BREZEANU AURELIA, BLINDU R.Effective Conservation programs involve the achievement of complementary approachessuch as in situ and ex situ strategies (Sarasan et al., 2006).The integration of different strategies is almost always necessary, the biotechnology usehaving benefits for the conservative purpose (Benson E., 1999, Bunn and Tan, 2002).Generally, based on plant cells totipotency capacity, in vitro tissues cultures conducted toplant regeneration through two main developmental processes: organogenesis and somaticembryogenesis.Somatic embryos are living structures similar to zygotic embryos, with bipolar structure andwithout vascular connection with the explant tissues.ES represents the ideal way for clonally mass propagation and to preserve plant material onmedium term, long-term or for synthetic seeds production.Another important advantage of SE is that somatic embryos are more genetic stablecomparing with the shoots. Somatic embryogenesis occurred generally as an effect of hormonessignals which determines in a single cell or cells group, the dedifferentiation and the capacity toactively divide.In the case of direct somatic embryogenesis, the differentiated tissues of the explants changetheir status; the embryogenic competence is induced through growth factors signals.Besides of the large scale propagation, somatic embryos can be used for production ofsynthetic seeds through the encapsulation in alginate.Synthetic seeds are defined as "encapsulated somatic embryos” which functionally mimic seeds andcan develop into seedlings under sterile conditions". The direct delivery of encapsulated material cansave many subcultures to obtain plants and also eliminate the difficult stage of acclimatization of invitro plants.In our experiment, we studied the effect of mannitol added in the culture media primary used asgrowth retardant for medium term cultures.We developed a protocol for somatic embryogenesis induction in moderate osmotic stress conditionas a tool to propagate and to obtain synthetic seeds in several Dianthus rare species from RomanianFlora.The somatic embryogenesis process was proved and characterized at cytological andhistological level.In several Dianthus rare species from Romanian Flora: Dianthus tenuifolius Schur., Dianthuscallizonus Schott et Kotschy, Dianthus spiculifolius Schur., Dianthus glacialis ssp. gelidus Schott,Nyman et Kotschy , Dianthus nardiformis Janka, Dianthus superbus ssp. superbus was established aprotocol for medium term- maintenace for conservative purpose based on the use of mannitol asgrowth retardant.The regeneration methodology in these species was previously established and improved(Holobiuc & Blîndu, 2006, 2009), these species have a good in vitro reactivity and rapid growth rate,for medium term conservation was efficiently used a slow growth method.35

To detect the alterations occured at the cellular level after osmotic stress exposure, cytologicalinvestigations were made.Electron microscopy analyses were performed in order to detect the effect of mannitol atcytological level in Dianthus glacialis ssp.gelidus regeneratives cultures in presence of a moderatelevel of this osmolite (0.32 M) after different intervals of time (1 month, 2 months, 6 months, 1 year,2 years of cultures).For somatic embryos developmental stages analyses semi-fine sections and histologicalsamples were done. The analysis of fresh material was made at 100X, 256X, 640 X.Somatic embryogenesis were also analysed in fresh material starting to the second months ofculture and continuing after several months using the light microscopy.Synthetic seeds production was made using detached Dianthus mini regenerants derived fromsomatic embryos induced on 0.32 and 0.49 M mannitol added medium, having about 1-2 mm heightand developed apical and basal meristem.This kind of plant material can be used for ex situ preservation on medium or long-termperiod or for direct sowing.P.I.6 IMPACT OF CRYOPRESERVATION ON BIOCHEMICALPARAMETERS OF IN VITRO CULTURED HYPERICUMRUMELIACUM BOISSK. DANOVA 1 , M. URBANOVÁ 3 , M. SKYBA 3 , E. ČELÁROVÁ 3 , M. STEFANOVA 2 , D.KOLEVA, V. KAPCHINA-TOTEVA 1*Key words: Hypericum rumeliacum, in vitro culture, cryopreservation, secondary metabolites,physiological status, histological structureThe Balkan endemic Hypericum rumeliacum Boiss. was subjected to cryopreservation experiments inorder to assess the impact of cryo-storage on its biochemical and physiological characteristics.Comparison of the levels of phenolic and flavonoid compounds, some physiological parameters andhistological characteristic of photosynthetic apparatus of regenerated H. rumeliacum aftercryopreservation in comparison with the unfrozen controls shows restoration of the biosyntheticcapacity and physiological status of regenerants in comparison to their control plants.1 Department of Plant Physiology, Faculty of Biology, St. Kl. Ohridski Sofia University, 8 Dragan Tzankov blvd,Sofia 1164, Bulgaria * e-mail: veneta@biofac.uni-sofia.bg, k_danova@abv.bg2 Department of Botany, Faculty of Biology, St. Kl. Ohridski Sofia University, 8 Dragan Tzankov blvd, Sofia 1164,Bulgaria3 Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Slovakia36

P.I.7 MICROPROPAGATION AND BIOLOGICAL ACTIVITY OFLEONURUS CARDIACAM. DIMITROVA 1 , M. HRISTOVA 1 , D. DRAGOLOVA 1 , V. KAPCHINA-TOTEVA 4*Key words: micropropagation, phenolic substances, biological activityLeonurus cardiaca . was successfully propagated in vitro on MS medium. Influence of differentconcentrations (0,1-1,0 mg/l)of benzyladenine and Indole-3-butyric acid stimulated micropropogationand callus genesis. Low concentrations stimulated the number of shoots and explants while higherconcentrations stimulated callus genesis. The pharmacological effects of Leonurus cardiaca l. havemainly been attributed to the polyphenolic contents. The quantity of polyphenolic substances wasstudied in relation to the growth regulators applied. Antioxidant and antibacterial activity of in vivoand in vitro propagated plants was evaluated.P.I.8 INFLUENCE OF HYDRIC STRESS ON SOME VALUABLEBIOCOMPOUNDS IN CEREALS GRAINSGABRIELA LUTA, EVELINA GHERGHINA, FLORENTINA ISRAEL-ROMING,DANIELA BALAN, GEORGE MUSATFaculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine59 Marasti Bd., Bucharest, RomaniaDrought stress is a worldwide production constraint for cereal crops, so that became a seriousproblem in that regions where maize, wheat, barley and other small-grained cereals are part of thestaple diets.The purpose of the present work was to evaluate the effect of the hydric stress on the quality of themaize yield. Therefore some biochemical compounds were analyzed in grains of maize cultivatedunder different watering conditions. Twenty-five maize hybrids cultivated in two agriculturalRomanian regions were subjected to optimal and stress treatment of watering during the growthperiod. Content in dry matter, in proteins, starch and carotene were measured postharvest in thegrains of maize using proper biochemical methods.As a result, it was noticed that the exposure to the hydric stress caused a significant decrease of thedry matter accumulation to all the studied maize hybrids. Also, the hydric stress induced a decreaseof content in starch, but an increase of the content in proteins and in carotene was registered. Somedifferences between the maize hybrids were noticed.37

P.I. 9 INVESTIGATIONS OF CHAENOMELES JAPONICA(THUNB.) LINDL. IN BULGARIATEODORA MIHOVA*, PENKA MONDESHKA * AND VIOLETA KONDAKOVA*** Research Institute of Mountain Stockbreeding and Agriculture – Troyan, str. “V. Levsky” 281,Troyan 5600, Bulgaria** AgroBioInstitute, Blvd. “Dragan Tsankov”8, Sofia 1164, BulgariaKey words: Chaenomeles, in vitroThe characterization of genetic resources of Chaenomeles japonica (Thunb.) Lindl, spread in theregion of Central Balkans, aims to assess its complex of biological, biochemical, biotechnology andmetabolic properties.The fruits of this plant species are characterized by a rich content of biologically active substanceshaving greater importance to human health and food industry. Determination of metabolicparameters of the spectrum depending on the environmental area, the method of generation andstorage of fruit is defined as self-direction at the Research Institute of Mountain Stockbreeding andAgriculture – Troyan.P.I.10 PRODUCTION OF POTATO MINITUBERS THROUGHHYDROPONIC TECHNIQUEANDREEA NISTOR, NICOLETA CHIRU, DIANA KARACSONYI*,GHEORGHE CAMPEANU, NICOLAE EUGEN ATANASIU***Department of vegetal tissue cultureNational Institute of Research and Development for Potato and Sugar Beet Brasov, ROMANIA** University of Agronomical Sciences and Veterinary Medicine – Bucharestnistorandreea06@gmail.comKey words: biotechnology, nutritive solution, minitubers, substrate, acclimatizationIn recent years, hydroponics has proven to be a very successful strategy for the productionof pre-base seed potatoes. Hydroponics methods facilitate the adequate supply of nutrients to plants.We used as mineral substrates: perlite and expanded clay granules. In this work, we present thebehavior of different potato varieties (Ostara, Roclas, Chistian) on 2009, on hydroponic system andwe presented a comparison of plant behavior on perlite and expanded clay granules. The best resultswere registered at semi-early variety- Christian, followed by early variety Ostara.38

P.I.11 THE ANALYSE OF DIFFERENT POTATO VARIETIES ONPRE-BASIC SEED PRODUCTION PROCESS, INGREENHOUSE, USSING MINITUBERSANDREEA NISTOR, NICOLETA CHIRU, DIANA KARACSONYI*,GHEORGHE CAMPEANU, NICOLAE EUGEN ATANASIU*** Department of vegetal tissue cultureNational Institute of Research and Development for Potato and Sugar Beet Brasov, ROMANIA** University of Agronomical Sciences and Veterinary Medicine – Bucharestnistorandreea06@gmail.comKey words: plantlets, cuttings, minitubers yield, variety, Solanum tuberosum L.The aim of the research was to determine performance of plantlets derived from “in vitro”propagated plantlets of five potato cultivars/three years.Minitubers represent a way to produce the pre-basic material, by transplanting the vitroplants (witha length of 5-7 mm) on protected environment (greenhouse, tunnels “insect proof”). The density ofplants/m 2 may vary 125 – 200 – 250 on a substrate with peat and manure (1:1). The minitubers are abiological source which is helpful in case of necessity, on initiation of a new culture “in vitro”, or forclones conservation. Minitubers production may vary in function by density and cultivar, from 2 to 4-5 minituber/plant.P.1.12 PRELIMINARY RESULTS CONCERNING THE GRAPEVINEBACTERIAL CANCER*S.C.D.V.V. ODOBESTI** I.N.C.C.F. BUCURESTIAURELIA PODOSU*, MARIA PAMFIL**, GH. MIHU*, ILEANA STOIAN*,LACRAMIORA MIRON*, MARIOARA BOSOI*, IONICA BOSOI*Key words: Grapevine, bacterial cancer, biopreparates, bactericide action, bacterial cancerThe grapevine bacterial cancer, due to its particular aspect, has been known for a longtime, and it was described for the first time in Germany, in the year 1822 by P. Horter and in 1853 inFrance by E. Fabre and F. Dunal, without its aetiology being known (Faivre-Amiot 1984). In 1910Hedgcock determined the bacterial nature of the B.Tumefaciens pathogen. The bacterial cancercaused by Agrobacterium radiobacter pv.tumefaciens (Smith et Townsend), has been known to affecta signifficant number of plants ever since the Antiquity, leading to signifficant damage. In ourcountry, the grapevine cancer due to Agrobacterium tumefaciens has been reported in the year 1929in the Arges county by Traian Savulescu. The disease is spread all throughout vineyards andviticultural centres in our country, and it has been signaled as affecting sensitive varieties, especiallyin the areas which constantly record minimum temperatures over their limit of endurance to frost.Currently, studies are being conducted concerning an integrated and rational control of thebacterian cancer of the grapevine, a higher biological protection with the end purpose of elliminatingtoxic and pollutant pesticides. In the attempts to limit and prevent of tumorgenesis given byAgrobacterium tumefaciens in the grapevine, the efficacy certain biological products based onstrands of Bacillus subtilis, made at I.N.C.D.C.F. Bucureşti, has been tested.39

P.I.13 INFLUENCE OF SOIL MOISTURE AND SEED TREATMENTON GERMINATION AND INITIAL DEVELOPMENT OF MANGOLDShumen University “Ep. Konstantin Preslavski”SVILEN RAYKOVKey words: seed, mangold, soil moisture, overall polish humidityUnder controlled conditions in 2006 was conducted an experiment with seed treated in different waysin levels of soil moisture, to establish their influence on germination and initial development of theseed. The results show that adequate soil moisture for this purpose is 70-90% of the OPH / overallpolish humidity. Here the time of germination is reduced by 5-12 days, which is of great importancefor agricultural production.At this humidity the plants grow and develop faster.The higher soil moisture - 80-90% of the OPH does not lead to etiolation of plants.P.I.14 SPATIALITY AND DURABILITY OF TILLAGE INFLUENCE ONTHE BULK DENSITY OF LEACHED SMOLNITSAShumen University “Ep. Konstantin Preslavski”IVAN DIMITROV, SVILEN RAJKOVIn a field experiment was studied the influence of the tillage systems in the rotation on thebulk density of Leached Smolnitsa (Haplic Vertisols, FAO). From the study was established that thepacking effect of the soil tillage machines is distributed along the whole plough layer, but the mostsignificant it is in the zone located immediately under the tillage depth. With the increase of the landuse period tendencies of overpacking are manifested. The main tillage of the spring crops and thetillage before sowing of the winter cereals are the basic regulators of the bulk density changes, but ina lesser degree the additional tillage influences also the spatiality and the durability of changes in thevalues of the bulk density.40

P.I.15 PTR-MS DETECTION OF NEPETALACTONE IN SHOOTCULTURES OF THREE NEPETA SPECIES GROWN UNDERDIFFERENT CARBOHYDRATE SOURCEJ. NESTOROVIĆ *5 , D. MIŠIĆ * , B. ŠILER * , S. ŽIVKOVIĆ * ,A. STOJIĆ ** , M. PERIŠIĆ ** D. GRUBIŠIĆ ** Institute for Biological Research "Siniša Stanković", Bulevar Despota Stefana 142, 11060 Belgrade, Serbia** Institute of Physics, Pregrevica 118, 11000 Belgrade, Serbia( * jasmina.nestorovic@ibiss.bg.ac.yu)Key words: PTR-MS, nepetalactone, Nepeta rtanjensis, Nepeta nervosa, Nepeta sibirica, NepetanervosaProton Transfer Reaction – Mass Spectrometry (PTR-MS) offers the possibility of sensitive volatileorganic compound (VOCs) detection without sample preparation or chromatography and is thereforea suitable tool to track the dynamics of VOCs emission. PTR-MS has wide application in medicineand environmental research. Monitoring of VOCs emission could be used in the control of food, andexamples will be shown on the ripening/aging process of fruit, on VOC emissions from coffee, and onthe quality control of meat.In the present study we describe the possibilities of PTR-MS use in the detection of volatilecompounds under in vitro conditions. The identification of nepetalactone in the atmosphere of glassjars, in which shoot cultures of three Nepeta species were grown, was performed. The effect ofdifferent carbohydrate source in culture medium on the accumulation of nepetalactone in shootcultures of N.rtanjensis, N. sibirica and N.nervosa was investigated. Furthermore, the release of thesemonoterpenoid lactones from the surface of shoot cultures was also examined.P.I.16 INFLUENCE OF TEMPERATURE AND PH ON MYCELIALGROWTH OF SOME PLEUROTUS OSTREATUS STRAINSA.V. ZAGREAN 1 , GH. P. NEGULESCU 2 , GH. CAMPEANU 2 , N. ATANASIU 21)SC MYCO-Z TECHNOLOGY SRL2)THE UNIVERSITY OF AGRONOMIC SCIENCES AND VETERINARY MEDICINE, BUCHARESTKey words: Pleurotus ostreatus, mycelial growth, culture media, pH, temperature.Five strains/isolates of Pleurotus ostreatus were examined for in vitro mycelial growth on three typesof nutritive media: malt extract-agar (MEA), potato dextrose-agar (PDA) and wheat extract-agar(WEA). The influence of medium type and pH (5.5, 6.0, 6.5, 7.0) on the mycelial growth at differenttemperatures (18, 20, 22, 24, 26, 28, 30°C) was studied. The results of the regression analysis appliedto obtained data showed that the optimal mycelial growth rates at 7 th day of incubation wererecorded between 26°C and 30°C for all media, strains and pH values.The multispore culture 421/Iand the commercial strain P80 provided the fastest mycelial growth on PDA adjusted to pH 6.541

P.I.17 IN VITRO PROPAGATION OF BULGARIAN LINE ECHINACEAPURPUREA L.TS. DZANFEZOVA 6 , A.DZURMANSKI 7 , ZH.YORDANOVA 1 , V.KAPCHINA-TOTEVA 1∗Key words: secondary metabolites, in vitro propagation, antioxidant activityEchinacea purpurea L. is one of the most common medicinal plants used as an immunostimulant.Echinacea, better known as purple coneflower, has received a global attention because of itsincreasing medicinal value. The production of new varieties and higher quality products fromEchinacea spp. requires a greater understanding of the regulation of plant growth and the productionof specific secondary metabolites. The current studies were designed to generate in vitro propagationprotocols for selected Bulgarian line E. purpurea L. Seeds are an important explants for establishingof genetic stable in vitro cultures. Different methods for seed sterilization including surfacesterilization with Domestos have been adopted. For in vitro germination of Echinacea seeds halfbasal MS media components are sufficient. The influence of different concentrations of plant growthregulators on in vitro propagation of Bulgarian line of E. purpurea, antioxidant activity and phenolscontent of different extracts was investigated.6 Department of Plant Physiology, Faculty of Biology, St. Kl. Ohridsk,i Sofia University, 8 Dragan Tzankov blvd,Sofia 1164, Bulgaria7 Institute of Roses, Essential and Medcinal Cultures, Osvobozhdenie 49 blvd., 6100, Kazanlak, Bulgaria42

P.I.18 INFLUENCE OF CRYOPRESERVATION ON THE BASICPHYSIOLOGICAL INDECES IN PROPAGATED IN VITROORTHOSIPHON STAMINEUS BENTHZH. P. YORDANOVA 8 , M. URBANOVA 9 , E. CHELAROVA 2 , TS. GANEVA 10 , D.KOLEVA 3 , V. M. KAPCHINA-TOTEVA 1∗Key words: micropropagation, cryopreservation, phenolic substances, stress markersInterest in the physiological role of bioactive compounds present in plants has increased dramaticallyover the last decade. Orthosiphon stamineus Benth is a very popular and widely used medical plant inSouth-East Asia, because of its broad range contents of bioactive compounds. Cryopreservation is analternative method for the long-term conservation of plant genetic resource. Establishment of suitableprotocols for in vitro propagation and long term conservation gives a reason for development of highproductive line in laboratory conditions and extraction of valuable secondary metabolites. In thepresent study we did not establish any considerable changes in the morphological, histological andphysiological status of plants before and after cryopreservation. We determined higher phenoliccontent by comparison with ex vitro adapted plants, which presumes suitable protocol forcryopreservation of Orthosiphon stamineus.P.I.19 THE EFFECT OF NaCl AND SUGARS ON SECOIRIDOIDGLUCOSIDES PRODUCTION IN ROOT CULTURES OF TWOCENTAURIUM SPECIES (FAM. GENTIANACEAE)D. MIŠIĆ 11* , B. ŠILER * , J. NESTOROVIĆ * , D. GRUBIŠIĆ *Key words: spiked centaury, yellow centaury, secoiridoid glucosides, NaCl, sugarsYellow centaury (Centaurium. maritimum (L.) Fritsch), and spiked centaury (Centaurium. maritimum(L.) Fritsch) from fam. Gentianaceae inhabit various saline soils of Western Europe andMediterranean region. Like other Centaurium species, they are a rich source of secoiridoidglucosides, among which the most important are swertiamarin, sweroside and gentiopicrine. Theycould be used as a substitute of C. erythraea which is officially listed in the pharmacopoeias of manycountries. Furthermore, on the basis of their ecological demands for saline soils, they could besuccessfully cultivated in the coastal regions and various arid areas.Root cultures are a good alternative for the large scale production of secoiridoid glucosides, whichhave a great application in pharmaceutical and food industries. Here we present our resultsregarding the effect of NaCl and sugars on secoiridoid glucosides production and accumulation inroot cultures of spiked and yellow centaury.8 Department of Plant Physiology, Faculty of Biology, St. Kl. Ohridski Sofia University, 8 Dragan Tzankov blvd,Sofia 1164, Bulgaria9 Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Slovakia10 Department of Botany, Faculty of Biology, St. Kl. Ohridski Sofia University, 8 Dragan Tzankov blvd, Sofia1164, Bulgaria∗ corresponding author e-mail: veneta@biofac.uni-sofia.bg* Institute for Biological Research “Sinisa Stankovic”, Boulevar despota Stefana 142, 11000 Belgrade, Serbiadmisic@ibiss.bg.ac.rs43

SECTION III: FOOD BIOTECHNOLOGYP.III.1 MODIFFICATIONS OF THE TARTARIC COMPOUNDSSOLUBILITY DURING MALOLACTIC FERMENTATION OFSOMERED AND WHITE WINES1 ODĂGERIU G., 2 BĂLĂNUŢĂ A., 1 NEACŞU I., 1 ZAMFIR I. C.,3 COTEA V. V., 4 RUSU E., 5 VACARCIUC L., 3 MORARU I.,1 Research Centre for Oenology – Iaşi Branch of Romanian Academy, odageriu@yahoo.com2 Technical University of Republic of Moldova, e-mail: balanutaanatol@mail.md3 University of Agricultural Sciences and Veterinary Medicine of Iaşi4 Scientifical-Practical Institute of Horticulture and Alimentary Technologies, Republic of Moldova5 State Agrarian University from Republic of MoldovaKey words: red and white wines, malolactic bacteria, malolactic fermentation, tartaric compoundssolubility, saturation temperatures, tartrates excessThis scientific paper presents data regarding the tartaric compounds solubility (acidpotassium tartrate-KHT and neutral calcium tartrate-CaT) during the malolactic fermentation ofsome red and white wines concomitantly with evolution of total, volatile and real acidity (pH), as wellas of some composition characteristics: tartaric, malic, lactic and citric acids, potassium andcalcium. The trials were conducted on seven different wines from the harvest of year 2007: two whitewines obtained from Rkaţiteli and Zghihară varieties and five red varieties (Burgund mare, Băbeascăneagră, Cabernet Sauvignon, Merlot and Fetească neagră). We have investigated some winesaccording to their pH values (3.085, 3.135, 3.264, 3.352, 3.422, 3.470, 3.505) and total acidity (9.96,8.84, 7.88, 7.56, 7.25, 6.94, 6.63 g/L C 4 H 6 O 6 ). The investigations were carried out in laboratoryconditions, during 40 days. For starting the malolactic fermentation, at the end of wine alcoholicfermentation (after 7-15 days), selected malolactic bacteria (SMB) from the Biolact aclimateé(Oenococcus oeni class) assortment were added in wines as leaven. Tartaric compounds solubilitywas evaluated based on concentration products (P C and P CT ), solubility products (K ST and K S ), KHTand CaT excess at – 4 °C and of theoretical saturation temperatures (T TS ) of KHT and CaT. Duringthe malolactic fermentation we found modifications of tartaric compounds solubility, due to pHvalues increase. Generaly, increase of concentration values of acid tartrat and neutral tartrat ionstakes place, that induce higher values of concentration products, with a direct implication in increaseof theoretical saturation temperatures. Excess of KHT and that of CaT, calculated for temperature of-4 ºC (refrigeration temperature of wines) had a different evolution: that of potassium acid tartratdiminshed (as normally), and that of calcium neutral tartrat increased, contrary to a normalevolution of this in similar processes. Obtained data can be useful for specialists in oenology practicefor a more rigurously control of wines during the malolactic fermentation and afterwards.44

P.III.2 STUDY OF COLOR EVOLUTION DURING SHELF LIFE OFAPPLE PUREE TREATED WITH PULSED ELECTRIC FIELDMIHAELA GEICU * , MONA POPA * , AMALIA MITELUŢ * ,PETRU NICULIŢĂ * , RADU CRAMARIUC *** Faculty of Biotechnology, Dept. of Industrial Biotechnologies, USAMV Bucharest, Romania, e-mail:mihaela_geicu@yahoo.com** Electro-technologies Research Center – Bucharest, Romania, e-mail: raducramariuc@yahoo.comKey words: pulsed electric field treatment, color changes, shelf life, apple puree.Nowadays, the consumers demand high quality and safety products with “fresh like” taste.Thermal pasteurization is quite efficient in preventing microbial spoilage of food products but theapplied heat may also cause undesirable biochemical and nutritious changes which may affectoverall quality of the final product. High voltage pulsed electric fields (PEF) treatment, which is analternative method of pasteurization that do not include aggressive heating, have been investigated inthis study, in order to obtain a safe product for human consumption, but with sensory attributessimilar to the untreated minimally processed fruits. PEF treatment may inactivate micro-organismswith only a small increase in temperature, simultaneously providing consumers with safe, nutritious,and fresh-like quality foods. PEF treatment is conducted at ambient temperature for a short time (inmicroseconds), and energy lost due to heating of foods is minimized.In this study the apple puree, prepared from Golden Delicious fruits, was pasteurized usinga pulsed electric field (PEF) treatment and stored at chilling temperature, at about 4 o C. During theshelf life of apple puree treated with PEF, color was measured using MiniScan XE Plus (Hunter Lab,USA) spectrocolorimeter. The color parameters were expressed as CIE Lab values, respectively a* -green to red, b* - blue to yellow and L* - black – white indices. This color indicators, also known astristimulus values, and the reflectance at specific wavelengths, between 400 -700 nm, have been usedto express color data.45

P.III.3 THE CHARACTERIZATION OF THE CU, ZN, CO, CD ANDFE CONTENT OF LACTOBACILLUS AND BIFIDOBACTERIUMSTRAINS USED FOR PROBIOTIC PRODUCTIONANA DESPINA IONESCU*, CASARICA ANGELA*, ELENA BOCA*, EUGENIAMOCANU*, RAMONA IONELA COJOCARU*, RUSU NICOLETA*, DOBRENICOLETA*, IONICA ILEANA*, ADRIAN VAMANU**, EMANUEL VAMANU*** National Chemical-Pharmaceutichal for Research and Development Institute,** USAMV-BucharestKey words: Lactobacillus strains, trace elements, probiotic productionThis paper presents the biotechnological studies concerning the characterizationof the trace elements from the biomass of Lactobacillus and Bifidobacterium strains used forprobiotic production . A set of chemical and microbiological analyses was established, in orderto obtain a high rate of cellular multiplication .The Lactobacillus and Bifidobacterium strains are known as importantcomponents of the human intestinal microflora. Such microorganisms have an importantcontribution in keeping an adequate hygiene and in increasing the immunity, having in the sametime, an anti-tumor effect.In order to obtain high yields of bacterial biomass with some selected strains ofLactobacillus and Bifidobacterium , various cultivating conditions such as the composition ofmedia were studied, and the purpose of this experiments was to verify the valuable traceelements' presence. The copper, zinc, cobalt, cadmium and iron total concentrations weredetermined by A Perkin Elmer Elan DRC-e inductively coupled plasma spectrometer ICP-MS.The preinoculum medium contained malt extract, the inoculum and production media, one of thefollowing: glucose, peptone or yeast extract.The obtained results were compared to the international recommended data, inorder to establish the possibilities of using these strains for the human treatment .In conclusion, from the trace elements controlled during our studies, the mostinfluential factor showed to be the iron, which can explain the important effect of these bacterialstrains on the human health.46

P.III.4 THE TECHNIQUES OF MARKETING PERFORMANCEINCREASE BY OPTIMIZING THE SUPPLY CHAIN STRUCTURE1 USAMV BucharestG. MARGARIT 1 , L. VISAN 1 , C. RADU 1 , A. EPURE 1Key words: marketing, performance, supply chain, direct distribution, optimizationIn the modern trends of marketing mix, there are preferred more and more the shortdistribution channels and the forms of direct sales while self-supplying is expanding. This tendency isa result of a large share of the total expenditure distribution in the total selling price becausesometimes these products get to the consumers at inaccessible prices, even though the manufacturerpractices competitive prices.In reality, besides the product circulation between producers and consumers, thedistribution process contains more economic flows as: transactions (negotiation flow), informationflow, promotional flow, product flow.The multi-channel marketing provides for the manufacturers three advantages: bettercoverage on the market, lower distribution costs, higher "personalization" of the sales process.The selection of the channels is set up by a thorough analysis of the market, being in fact,the process that determinates the distribution channels. This analysis carried out in the marketingdepartment, starts with the final consumer and ends up with the manufacturer, because distributionchannels dependent on the consumer demand and preferences. The "customer" factor refers to theconsumers’ number and their territorial distribution, the buying habits, the purchasing averagevolume and the reaction to different methods of value.The selection of the distribution channel type (long, short or straight) is an importantdecision and it is considered the foundation of the distribution policy. This depends on the type of theproduct, commercial usage, local buying habits, the amount and frequency of sales, the profitabilitydistribution organization.P.III.5 EIGHT CELL DISRUPTION METHODSFOR THE OBTAINING OF SPENT BREWER ’ S YEAST CELL WALLMARINESCU GINA*, STOICESCU ANTONETA*** ,,Elena- Doamna” Galati, Food Industry College, Domneasca Street 169**„Dunarea de Jos” University Galati, Food Science and Engineering Faculty Domneasca Street 111Key words: Cell wall, spent yeast, autolysis, disruption, ball grindingThe work presents eight yeast disruption methods for the obtaining of spent brewer’s yeastcell wall: plasmolysis, sonication, thermolysis, chemical treatment, freezing- defreezing, crushing inthe mortar with sand, ball milling and autolysis. The efficiency of the methods was established bymicroscopic examination, the cells viability assay and soluble protein content assay fromsupernatants that result by yeast biomass centrifugation. It has been demonstrated that autolysisleads to the highest efficiency. It has been drawn a comparison between yeast disruption methods fortwo spent yeast generations and an elaborate study of mechanical breakage with balls mill, too.47

P.III.6 CORRELATION OF ANTIOXIDANT PROPERTIES OFSOME VEGETAL EXTRACTS OBTAINED FROM HYPERICUMPERFORATUM L., EVALUATED BY CHEMILUMINESCENCEAND LIPID PEROXIDATION ASSAYCORNELIA NICHITA*, GEORGETA NEAGU-CARAENE * ,VIRGINIA VULTURESCU*, CRISTINA BAZDOACA*,RADU ALBULESCU*, NICOLETA BADEA**, ILEANA IONICA** National Institute for Chemical-Pharmaceutical Research and Development, Bucharest,Romania , e-mail: cornelianichita@yahoo.com** University POLITECHNICA of Bucharest, Romania.Key words: chemiluminescence, antioxidant activity, Hypericum perforatum L., pharmacologicaltestsThe importance of the vegetal antioxidants is justified by their action over the free radicals(FR). Numerous scientific studies have emphasized that the excessive production of oxygenreactive species (ROS) or FR is involved in human diseases pathogenesis. By the increasedreactivity of the molecules, the FR may determine multiple biological disturbances of somesub-layers such as proteins, lipoproteins, nucleic acids, glucides, polyunsaturated fat acids.Furthermore, FR role as secondary messengers in inducing molecular processes was alsodemonstrated.This study presents the evaluation of antioxidant activity of some vegetal selective extractsobtained from Hypericum perforatum L . Antioxidant properties was studied using thechemiluminescence technique and ex vivo lipid peroxidation assay. A correlation betweenantioxidant activity and phenolic/flavonoidic content was observed for all the selectiveextracts, and a parallel behaviour with the antioxidant activity determinated by ex vivolipid peroxidation assay and in vitro chemiluminescence tests was also noted.The results obtained recommending the selective vegetal extracts from Hypericumperforatum L. species for prophylactic and therapeutic purposes.48

P.III.7 ANTIOXIDATIVE CHARACTERISTICS OF CALENDULAOFFICINALIS L. EXTRACTS DETERMINATED BYCHEMILUMINESCENCE TEHNIQUECORNELIA NICHITA*, GEORGETA NEAGU * , CRISTINA BAZDOACA*,VIRGINIA VULTURESCU*, RADU ALBULESCU*,MARIA GIURGINCA**, NICOLETA DOBRE** National Institute for Chemical-Pharmaceutical Research and Development, Bucharest,Romania , e-mail: cornelianichita@yahoo.com** University POLITECHNICA of Bucharest, Romania.Key words: chemiluminescence, antioxidant activity, vegetal extracts, Calendula officinalis L.Antioxidants play an important role in preventing or alleviating chronic diseases byreducing the oxidative damage to cellular components caused by reactive oxygen species(ROS). Many investigations have correlated the pharmacodynamic properties of some plantextracts with their antioxidant activity and the capacity to defend the organism againstoxidative stress. Among the plants of interest, the species Calendula officinalis L. is alsosituated, whose chemical composition is rich in saponosides, carotenoids, resins, essentialoil, flavonoids and polyphenols components.The work investigates through chemiluminescence tehnique the antioxidant characteristicsof some vegetal extracts obtained from Calendula officinalis L.Experimental data emphasized the structural complexity and high antioxidative capacity.The dependence of the antioxidant activity from the content in carotenoids and flavonoidiccompounds has been revealed.49

P.III.8 PRELIMINARY CHARACTERISTICS OF MYOFIBRILLARPROTEINS OBTAINED FOR EDIBLE BYOFILMS REALISATIONFLORICEL CERCEL*, LIVIA PATRAŞCU*,PETRU ALEXE**, MARIANA STROIU****- PHD STUDENT**- PROFESSOR PHD***- RESEARCH ENGINEERUNIVERSITY „DUNAREA DE JOS” GALATI, FOOD SCIENCE AND ENGENEERING FACULTYKey words: myofibrillar proteins, protein functional properties, edible biofilms.There were obtained myofibrillar proteins by extraction with KCl and EDTA, after a previouscomparation with other extraction methods.Myofibrillar proteins were characterised in the lyophilized form for a better conservation andrepeatability. Lyophilized myofibrillar proteins properties were compared with other proteinsproperties involved in edible biofilm formation.Also from obtained liophilized myofibrillar proteins were realised biofilms.This study was performed within a doctoral program regarding myofibrillar protein characterisationin different sistems.P.III.9 VALORIZATION OF BIODEGRADABLE MATERIALSUSING BIOTECHNOLOGYMONA POPA 11 University of Agronomic Sciences and Veterinary Medicine Bucharest,Faculty of Biotechnology, RomaniaKey words: biodegradable composites, protein biomass, animal fodder.The big amount of plastic disposable being produced nowadays makes it imperative to identifyalternative procedures for making them biodegradable and to identify valorization techniques whichcan lead to useful compounds obtaining.This paper is a review which describes the innovative uses of consumed biodegradable waste as rawmaterials for protein biomass production that can be used as animal fodder. Particularly, compositematerials based on PLA, ECOFLEX and wood flour will be used as substrate for microbial growthand biomass formation.These new biodegradable composites would appear to offer an attractive low-cost material for fodderproduction and in saving money.This study is made in the frame of FORBIOPLAST EU project Forest Resource Sustainabilitythrough Biobased Composite Development, Project no. 212239 – FP7- KBBE.50

P.III.10 SCREENING OF RHODOTORULA STRAINS IN ORDER TOSELECT A GOOD PRODUCER FOR CAROTENOID PIGMENTSGEORGETA RADULESCU 1 , AURORA SALAGEANU 2 , AURELIA CHIRVASE 3 ,EUGENIA MOCANU 1 , IONELA COJOCARU 1 , GABRIELA SAVOIU 1 ,FLORENTINA DAMSA 1 , NICOLETA DOBRE 1 , ILEANA IONICA 11 National Institute for Chemical-Pharmaceutical Research and Development - ICCF, Bucharest,Romania2 National Institute for Research & Development in Microbiology and Immunology "Cantacuzino"Bucharest, Romania3 "Politehnica" University of Bucharest, Bioengineering and Biotechnology Department,Bucharest, RomaniaCarotenoids are a widely distributed group of naturally occuring pigments, essential tomicrobial, plant and animal life. They act as a potent antioxidants and scavengers for reactive singletoxygen and exhibit a protective role against manny diseases.A strong interest in natural pigments obtenance has raised due to the negative assessementof synthetic food dyes by the modern consumers.Many microorganisms, like bacteria, yeasts, fungi and algae are well known to accumulatecarotenoids as intracellular pigments and therefore they are subjected to a great number of studies inorder to select a good pigment producer. This was the purpose of our studies too.Yeasts belonging to the genera Rhodotorula existing into ICCF Microorganisms Collection( WFCC-232) are known to be able to produce carotenoids. The influence of carbon sources (glucose,sucrose, maltose, glycerol) on the biomas formation was studied for : Rhodotorula rubra ICCF 209,Rhodotorula rubra ICCF 210, Rhodotorula rubra ICCF 220 and Rhodotorula sp. ICCF 396 . Thecarotenoid production was investigated by spectrophotometric, thin-layer, and HPLCchromatographic methods applied on mixtures of extracted carotenoids. The concentrations of totalcarotenoids was calculated on the basis of a calibration curve of available comercial standard β-carotene and expressed as µg / g (β-carotene / dry cells weight ).The level of cell growth obtained in our studies and the carotenoid pigments productionwas in good accordance with those published in literature, therefore we are able to start the studiesconcerning the optimisation of cultivation conditions in order to enhance the cells growth and thecarotenoid pigments production.51

P.III.11 METHODS FOR OBTAINING ANTHOCYANINS FROMRASPBERRY PLANTSKAVITHA RAVICHANDRAN, NAY MIN MIN THAW SAW, HEIDI RIEDEL,CAI ZHEN ZHEN, IRYNA SMETANSKADepartment of Methods in Food Biotechnology, Institute of Food Technology and Food Chemistry, BerlinUniversity of Technology, Königin-Luise Str 22, 14195 Berlin.Key words: Anthocyanins, Raspberries, Pulsed electric fields (PEF), High pressure (HP).Anthocyanins are characterized by health- promoting properties and can be used aspharmaceutical, nutraceuticals and food additives. High concentration of anthocyanins inraspberries gives the great benefit. Our research concerns the development of methods for receivinganthocyanins from raspberries. Raspberries in aqueous solution are treated with pulsed electricfields (PEF), which enhances anthocyanin release. High pressure (HP) treatment at 50 to 100 MPresulted in the immediate increase of anthocyanin extraction. PEF and HP acts as a physical stressfactor for the membrane permeability. There was a significant increase in extraction of anthocyaninsfrom raspberries treated with high pressure at 50MP for 3 minutes and also in treatment with PEF at0 .5 KV/cm 50 pulses52

P.III.12 STRESS-INDUCED MODIFICATION OF THE LIPIDMEMBRANE COMPOSITION OF OENOCOCUS OENI CELLS:CHARACTERIZATION OF THE O. OENI CFA GENE BYHETEROLOGOUS COMPLEMENTATION OF A LACTOCOCCUSLACTIS CREMORIS CFA-DEFICIENT STRAINTHI-MAI-HUONG TO, COSETTE GRANDVALET,RAPHAELLE TOURDOT MARECHAL 11 Laboratoire de Recherche en Vigne et Vin, Institut Jules Guyot, Université de Bourgogne. 1 rueClaude Ladrey, 21078 Dijon Cedex, France.Email : TO Thi Mai Huong : maihuongbk@yahoo.comOenococus oeni (O. oeni) is the lactic acid bacterium mainly responsible for malolactic fermentationin wine-making. Its capacity to tolerate low pH and high ethanol content in wine resides, partly, in itsaptitude to maintain the integrity and the functionality of the plasmic membrane.The Cfa Synthase catalyses the conversion of Unsaturated Fatty Acids (UFA) to Cyclopropane FattyAcids (CFA). This cyclisation is one of the mechanisms involved in the modification of the UFA/CFAratio in the membrane in response to stress. Quantitative Real-Time PCR experiments wereperformed on the cfa gene of O. oeni, which encodes a putative CFA synthase. Results showed anincrease in cfa transcript level when cells were grown in the presence of ethanol or at low pH. Thefunction of the putative cfa gene identified in the genome of the O. oeni ATCC BAA-1163 strain wasinvestigated by means of heterologous complementation of a Lactococcus lactis cfa-deficient strain.Objective:Investigation of the O. oeni cfa gene encoding Cfa synthase:- Function of the cfa gene from O. oeni- Physical role of Cyclopropane Fatty Acids (CFA) in cell adaptationTo improving the biochemical and molecular biological knowledge of stress response in O. OeniMethods:1. Heterologous expression system in L. lactis cremoris MG1363- L.lactis ∆cfa construction by homologous recombination- Construction of the plasmid pGC20 including the O. oeni cfa gene which comes from the vectorpDL278- Transformation in L. lactis and L.lactis ∆cfa2. Analyses et comparisons of Fatty Acid profiles for 4 strains in growth media M17/7, M17/5,M17/7+Tween80 and M17/5+ Tween80 by Gas Chromatography (GC)3. ARNm expression level comparison between the O. oeni cfa gene and the L. lactis cfa gene by RTqPCR4. Viability comparisons of the 4 strains in ethanol shock (17% EtOH) and Acid shock (pH3)Results:1. Analysis by Gas Chromatography of the Fatty Acid membrane composition of the complementedstrain demonstrated the functionality of the O. oeni cfa gene. An increase in cycC19 :0 n-7 amountsand a decrease in C18 :1 n-7 was observed in the L. lactis complemented strain. However, theconversion ratio of UFA into CFA in the complemented strain remains lower than that of the wildtypestrain. The CFA synthase activity of O. oeni is induced by acidity.53

2. In the case ethanol of shock, the mutant is more sensitive than other strains. The complementedstrain recovers ethanol resistance. In the case of acid shock, we noted no significant difference insurvival between the mutant, the parental and the complemented strain.3. Thanks to the high copy number of plasmid pGC20, the transcription level of the O. oeni cfa geneis significantly higher than that of the L. lactis cfa gene (10 times in the case of pH7; 50 times in thecase of pH5). The transcription of the O. oeni cfa gene is induced by acidity (pH5)Conclusions:1. The cfa gene from O. oeni is functional: Expression of the cfa gene from O. oeni restores thesynthesis of CFA in L. lactis membrane; increases at the onset of the stationary phase and in the acidgrowth condition.3. Quantitative Real-Time PCR confirmed the high level of transcription of the O. oeni cfa gene.Hence, the partial complementation could be due to the heterologous expression of the O. oeni cfagene: a low translation level or a reduced enzymatic activity in relation with the host intracellularpH. Therefore, an enzymatic approach to studying CFA Synthase of O. oeni in vitro is beinginvestigated. Towards the same end, the study of the translation level is examined by means of theWestern Blot approach.4. The complementary strain partially recovers their viability after the acid and ethanol shocksReferences:Bligh,E.G. and Dyer,W.J. (1959). A rapid method for total lipid extraction and purification. Can.J.Biochem. Physiol. 37:911-917Chang and Cronan (1999). Membrane cyclopropane fatty acid content is a major factor in acidresistance of Escherichia coli. Mol. Microbiol. 33(2): 249-259.Grogan and Cronan (1986). Characterization of Escherichia coli mutants completely defective insynthesis of cyclopropane fatty acids. J. Bacteriol. 166(3): 872-877.Guianvarc'h, Drujon, et al. (2006). Identification of new inhibitors of E. coli cyclopropane fatty acidsynthase using a colorimetric assay. Biochim Biophys Acta 1764(8): 1381-8.LeBlanc, D., Lee, L.N. and Abu-Al-Jaibat, A. 1992. Molecular, genetic, and functional analysis of thebasic replicon of pVA380-1, a plasmid of oral streptococcal origin, Plasmid 28 , pp. 130–145.Hols, P., Ferain, T., Garmyn, D., Bernard, N., and Delcour, (1994). Use of homologous expressionsecretionsignals and vector-free stable chromosomal integration and engineering of Lactobacillusplantarum for a-amylase and levanase expression. Appl. Environ. Microbiol. 60: 1401- 1413.54

P.III.13 IMPACT OF STRESS CONDITIONS ON THE GROWTH OFLACTOBACILLUS ACIDOPHILUS IBB 801 AND PRODUCTION OFACIDOPHILIN 801MEDANA ZAMFIR, SILVIA GROSU-TUDORInstitute of Biology Bucharest, Splaiul Independentei No. 296, 060031 Bucharest,P.O. Box 56-53, Romania, e-mail: medana.zamfir@ibiol.ro, medana_zamfir@yahoo.comLactic acid bacteria (LAB) are traditionally used as starter cultures for the fermentation of foods andbeverages and therefore, they should resist to adverse conditions encountered in industrial processes(like freeze-drying, low and high temperatures, low pH, osmotic stress etc.). LAB contribute to thetexture, flavour, and aroma development in the food product, but also to spoilage retardation. Thepreservative effect is mainly due to the acidic conditions that these bacteria create in food duringtheir development, but they are also able to produce and excrete inhibitory substances, such asbacteriocins. These are proteinaceous antibacterial compounds that inhibit Gram-positive bacteria,particularly closely related species.Lactobacillus acidophilus IBB 801, a dairy strain with potential probiotic properties producesacidophilin 801, a new bacteriocin, with a quite narrow inhibitory spectrum, including otherLactobacillus strains, but also some Gram-negative bacteria (Escherichia coli Row, Klebsiellapneumoniae K 33 and Salmonella panama 1467).The influence of different stress conditions, such as low and high temperatures, the presence of salt orethanol in the medium, on the growth of Lactobacillus acidophilus IBB801 and the production ofacidophilin 801 was investigated in this study. The strain was able to grow up to 47°C, while highertemperatures were lethal. A slow growth was detected at 24°C, starting after 24h of incubation, andthe strain was able to survive at 10°C more then 48h of incubation. The protein profiles revealed byone dimensional SDS-PAGE showed at least four overexpressed and two repressed bands at lowtemperatures (10°C and 24°C) compared with the profiles at 37°C and 42°C. The bacteriocin activitywas the highest when the producing strain was grown at optimum temperature. However, at 10°C, theinhibitory activity showed a slight increase compared with the one reached at 24°C and 47°C. Thesame increase in activity was observed in the presence of low amounts of salt (5 to 10 g/l). Higherconcentrations of salt or ethanol addition to the growth medium had a negative effect on acidophilinbiosynthesis.55

P.III.14 IMMUNOMODULATORY EFFECTSOF A SELECTIVE EXTRACT OBTAINED FROMCrataegus monogyna Jacq.VIRGINIA VULTURESCU*, CORNELIA NICHITA*, GEORGETA NEAGU*,LUCIAN ALBULESCU**, RADU ALBULESCU*, MANUELA MIHALACHE**** National Institute for Chemical-Pharmaceutical Research and Development, Bucharest, Romania,e-mail: virginiavulturescu@yahoo.com** The„Victor Babes” National Institute, Bucharest, Romania*** „Victor Papilian” Faculty of Medicine, „Lucian Blaga” University of SibiuKey words: immunomodulatory, interleukines, proteomic, Crataegus sp.In this study we investigated the specific and differential expression of IL-2, IL-4, IL-6, IL-8,IL-10, GM-CSF and TNFα in Jurkat cells and whole blood cultures in response to treatment with aselective extract obtained from Crataegus monogyna Jacq. PHA, ConA and LPS were used for thestimulation of the proliferative response.In previous studies we investigated the chemical and antioxidant properties of 9 selectiveextracts obtained from hawthorn. All extracts produced positive responses in the total phenolic andtotal flavonoid assays. The extract designed P25 was consistently amongst the most effective samplein radical scavenging assays and was selected for the in vitro studies. SELDI-TOF-MS (Surface-Enhanced Laser Desorbtion/Ionization-Time-Flight-Mass Spectrometry) and bio-informaticsdatabase systems were subsequently employed for proteomics studies.Proteomic analysis showed increased expression of IL-2 and IL-8, after treatment with theselective extract. The expression of IL-2 is optimum in the presence of Con A and PHA. In thepresence of PHA, IL-2 production showed superior values (72.3 pg/mL) comparative with the control(64,5 pg/mL). The treatment with the selective extract induced the increased of IL-8 expression in thepresence of ConA, with superior values (210,3 pg/mL) to those of the control (178,1 pg/mL). Theproduction of IL-8 is decreased (50.1 pg/mL) in the presence of PHA but comparable with the normalcontrols (57.3 pg/mL). The results indicate the capacity of the selective extract to stimulate thedevelopment of the immune responses in Jurkat cells.The results have shown that the compounds from Crataegus monogyna extracts may conferimmunomodulatory activities. Among these different observations, we belive it is important toevaluate systematically the specific and multiple effects of Crataegus monogyna phytocompunds onhuman immune cells, at cellular and molecular levels.This study provides information on candidate target molecules and molecular signalingmechanisms for future systematic research into the immune-modulatory activities of an importanttraditional medicinal herb and its derived phytocompounds.56

SECTION IV: INDUSTRIAL AND ENVIRONMENTALBIOTECHNOLOGYP.IV.1 STUDIES CONCERNING ENZYMATIC ACTIVITY OFASPERGILLUS NIGER 401 AND TRICHODERMA REESEI 4122STRAINS USED IN HYDROLYSIS OF ENERGY CROPSANGHEL LAURA 1 , PETRE STELIAN MATEI 1 ,ANA MARIA NICOLAE 2 ,CAMELIA DIGUTA 1 , STEFANA JURCOANE 11 Microbial Biotechnological Centre “BIOTEHGEN”, Blvd Marasti, No. 59, Bucharest, ROMANIAE-mail: anghel_laura@yahoo.com.au2 University of Agronomical Sciences and Veterinary Medicine, Bucharest, Romania, StudentLignocellulosic biomass (especially agricultural residues) is known as an excellent carbon source forproduction of microbial enzymes. Several substrates such as wheat straw, maize straw, corn cob, etc.,may be used as carbon sources in the production of enzymes.In the present study, we investigated theefficiency of complex enzyme produced by Aspergillus niger -401 and Trichoderma reesei-4122strains from Microbial Biotechnology Center Collection, on the degradation of energy crops; theproduction of extracellular cellulase, amylase and xilanase in submerged fermentation of selectedstrain has been studied; Maize straw and corn cob uses as inducers or carbon sources increasehydrolytic enzymes production.The production of enzymes by the fungus was stimulated in media containing nitrogen in the form ofprotein and ammonia azotate; good results were obtained for production of cellulase by Aspergillusniger 401 strain and for xilanazes by Trichoderma reesei 4122; the optimum rate between proteinsource(peptone) and inorganic nitrogen source Na NO 3 was 0.5/0.3, respectively.57

P.IV.2 ISOLATION OF OXYGENIC PHOTOTROPHIC AND OXICHETEROTROPHIC BACTERIA WITH POTENTIAL FORGASOLINE CONSUMPTION1 Institute of Biology, 060031 Bucharest; 2 Ovidius University, Constanţa 9004703 Constanta Maritime University, 900663 ConstantaARDELEAN I.I 1, 2 , GHITA S. 2, 3 , SARCHIZIAN I. 2Key words: bacteria, autotrophy, heterotrophy, gasoline, microcosms, applicationsIn this paper we present our results concerning the isolation of phototrophic and heterotrophicbacteria from Black Sea and from mesothermal sulphurous spring (Obanul Mare, Mangalia). Ourresults argue that mixed cultures of marine heterotrophs and marine cyanobacteria seems to beimportant in the oxidation of gasoline. Gasoline oxidizing bacteria were isolated using microcosmsenriched in nutrients (0,005% ammonium acetate final concentration) and gasoline (5mL/1,5). Theability of heterotrophic bacteria to growth (exclusively) using gasoline as sole added carbon sourcewas checked by several passages in the same growing conditions. Oxygenic nitrogen fixing andanoxygenic cyanobacteria were isolated using specific growing media in the presence or absence ofan organic or inorganic nitrogen or carbon source.P.IV.3 EPIFLUORESCENT METHOD FOR QUANTIFICATION OFPLANKTONIC MARINE PROKARYOTES1 Institute of Biology, 060031 Bucharest; 2 Ovidius University, Constanţa 900470,3 Constanta Maritime University,900663 ConstantaARDELEAN I.I 1, 2 , GHITA S. 2,3 , SARCHIZIAN I. 2Key words: epifluorescence microscopy, planktonic prokaryotes, AO, automated image analysisIn this paper we present our results concerning the use of epifluorescence for quantification thebacterial cell in microcosms, as model systems of natural ecosystems. The main results are focusedon the optimization of fluorochrome (acridine orange - AO) concentration and time of contact withthe cell suspension as well as the choice of the dispersion method. In our hands the best results wereobtained using 5 µg/mL AO final concentrations for 15 minutes of coloration. As in naturalenvironments in some cases bacterial cells occurs as aggregates the dispersion of these aggregates isessential for the microscopic enumeration of individual cells on membranes filters (Millipore, 0.22µm); physical treatment (2 minutes of vortexing and 90 seconds of ultrasonication (bath) andchemical treatment (Tween-80, 10 µg/mL final concentration) gave very good results. The liquidsamples (fixed with formalin 2% final concentration) were stored 1, 2, 4 and 8 weeks respectively at4º C in dark before analysis. Bacterial cell enumeration was done by manual counting and by imageanalysis (CellC) and cell size was done by image analysis (ImageJ). In time, the microcosms show adynamic of cell density from 2,9x10 3 to 15,2x10 3 cells/ml. The results obtained by manual countingcorrespond with those obtained by automated image analysis. This method is rapid, cheap andaccurate and can be used on either unfixed and fixed (e.g. formalin) natural unpolluted or pollutedsamples (unpublished results) from Black Sea.58

P.IV.4 RESEARCHES CONCERNING INHIBITION OF ACTIVITYOF SOME MATRIX METALLOPROTEASESKey words: matrix metalloprotease, collagen, inhibitorsDANIELA BĂLAN*, FLORENTINA ISRAEL-ROMING*,GABRIELA LUŢĂ*, EVELINA GHERGHINA*Matrix metalloproteases (MMP) mediate the hydrolysis of the structural molecules from extracellularmatrix, but an imbalance between the active enzymes and their natural inhibitors leads to theaccelerated destruction of connective tissue, which is associated with diseases such as arthritis,cancer, multiple sclerosis and cardiovascular diseases.The purpose of the performed researches was to test the inhibition effect of some new low-molecularmasscompounds, hydroxamate derivatives of benzimidazole, in order to be used in biologicalsystems as therapeutic agents.The inhibition effect was estimated by determination of enzymatic activity of collagenase IA (EC3.4.24.3) on specific substrate (collagen type I) in the presence of the new tested compoundscomparativelly with two reference inhibitors (EDTA and o-phenantroline).Comparative analyze of the inhibition power of the new synthesized compounds (C1-C5) indicated agood inhibition effect, closed to the inhibition power proved by the reference inhibitors.59

P.IV.5 IN VITRO PROPAGATION AND QUALITATIVEDETERMINATION OF SECONDARY METABOLITES OFGENTIANELA PRAECOX (A. ET J. KERN) DOSTALT. BANJANAC * , D. KRSTIĆ * , T. JANKOVIĆ *** , M. DEVIĆ * ,B. VINTERHALTER * , D. GRUBIŠIĆ * *** Institute for Biological Research “Sinisa Stankovic”, Boulevar despota Stefana 142, 11000 Belgrade, Serbia, e-mail: tbanjanac@sezampro.rs** Institute for Botany, Faculty of Biology, Takovska 43, 11000 Belgrade, Serbia*** Institute for Medicinal plant Research “ Josif Pancic”, Tadeusa Koscuska 1,11000 Belgrade, SerbiaKey words: Gentianella praecox, in vitro propagation, High Pressure Liquid Chromatography(HPLC), xanthonesThe genus Gentianella Moench (Gentianaceae) comprises some 250 species growing in temperate ormountain habitats throughout the world. In traditional folk medicine of the Balkan area, Gentianellaspecies are often used as substitutes for related Gentiana species (G. lutea and G. punctata). In thetraditional medicine of South America, Gentianella preparations are used for treatment of digestiveand liver problems.Continuing our study of the Gentianaceae family from Serbia we established a protocol forin vitro propagation of Gentianella praecox (A. et J. Kern) Dostal, a biennial herb 10-45cm tall,widely distributed in the middle and central Europe. Methods for in vitro propagation offer aconvenient way for mass propagation of various plant species. Procedures for in vitro culture, shootregeneration and clonal propagation are important steps in the development of new bio-technologies.Xanthones are the main secondary metabolites of the genus Gentianella. In recent yearsxanthones have become more interesting since they display numerous pharmacological activities.Detection and identification of secondary metabolites of in vitro cultured plants was performed usingthe High Pressure Liquid Chromatography (HPLC).60

P.IV.6 ALTERATIONS IN ENZYME ACTIVITIES IN LEAVESAFTER EXPOSURE OF WHEAT PLANTS (TRITICUM AESTIVUM)TO 900 MHz ELECTROMAGNETIC FIELDMILENA DIMITROVA 1 , DANIELA DRAGOLOVA 1 ,MARGARITA KOUZMANOVA 21 Department of Plant Physiology, e-mail: madimitrova@yahoo.com2 Department of Biophysics and Radiobiology, e-mail: kouzmanova@biofac.uni-sofia.bgBiological Faculty, Sofia University, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaKey words: enzyme activity, 900 MHz electromagnetic field, mobile phone, plant leaves, wheatAccelerated and widespread use of different communication systems and modern electronicequipment increases exposure to radiofrequency electromagnetic fields (RF EMF) and raises seriousconcerns about the biological and health-related effects of RF radiation. Not only population isexposed to mobile phone radiation, but flora and fauna around base stations, which multiplycontinuously.The purpose of our study was to investigate the alterations in enzyme activities in leaves afterone hour exposure of wheat (Triticum aestivum) to 900 MHz EMF and their dependence on the timeelapsed after exposure. Whole plants were exposed for 1 hour to EM radiation from GSM mobilephone (carrier frequency 902 MHz, 2 W pulse output power) at 20 cm distance from the antenna.Isocitrate dehydrogenase (NADP-ICDH), malate dehydrogenase (MDH) and glucose-6-phosphatedehydrogenase (G6PDH) activities in leaves were measured immediately after the end of theexposure and 1, 2 and 24 hours later.Irradiation of plants induced different alterations in enzyme activities depending on the timeelapsed after irradiation. NADP-ICDH activity decreased at 1 st hour after the treatment, increased at2 nd hour and fell down the control levels again at 24 th hour after exposure. G6PDH activitiesdecreased immediately after exposure and remained down the control for one hour, than graduallyincreased and returned to normal at 24 th hour. MDH activity increased immediately after the end oftreatment and decreased 1 hour later.Alterations in the activities of these enzymes mean alterations in respiration and energysupply of cells and in case of alterations in NADP-ICDH and G6PDH activity – impact onantioxidant defence of organisms. In conclusion, although these results cannot be used to concludewhether exposure to mobile phone EMF could lead to any hazardous effect, they may be a steptowards further explanation of RF EMF effects on biological systems.61

P.IV.7 DOES 900 MHz ELECTROMAGNETIC FIELD INDUCEOXIDATIVE STRESS IN WHEAT PLANTS?DANIELA DRAGOLOVA 1 , MILENA DIMITROVA 1 , MARGARITA KOUZMANOVA 21Department of Plant Physiology, e-mail: madimitrova@yahoo.com2 Department of Biophysics and Radiobiology, e-mail: kouzmanova@biofac.uni-sofia.bgBiological Faculty, Sofia University, 8 Dragan Tzankov blvd., 1164 Sofia, BulgariaKey words: 900 MHz electromagnetic field, mobile phone, oxidative stress, plant leaves, wheatRecently, some research studies have found a relationship between radiofrequencyelectromagnetic fields (EMF) emitted by a mobile phone and oxidative stress. Plants display a highlyvaried developmental and biochemical responses related to stress adaptation. A common response tomost stressors is an increased production of reactive oxygen species (ROS) because stress conditionsdisturb cellular homeostasis between generation and neutralization of these highly reactivemolecules. If not scavenged, ROS can damage plants by oxidizing membrane lipids, proteins,photosynthetic pigments, nucleic acids. ROS can also act as signaling molecules and trigger a rangeof cellular responses important for stress tolerance. The strong control of ROS level in cells isachieved by the antioxidant defense system including various antioxidant enzymes.The aim of our work was to investigate the effect of mobile phone radiation on parameters ofoxidative stress: malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ) content in wheat leaves(Triticum aestivum). Whole plants were exposed for 1 hour to EM radiation from GSM mobile phone(carrier frequency 902 MHz, 2 W pulse output power) at 20 cm distance from the antenna.Concentrations of MDA and H 2 O 2 in leaves were measured immediately after the end of the exposureand 1, 2 and 24 hours later. Our results showed that there were no alterations in MDA levels after theone hour exposure to 900 MHz EMF during the investigated period. Weak, but statistically significantdecrease in H 2 O 2 concentration was observed at 1 st and 2 nd hour after the treatment.These data showed that 1 hour exposure to 900 MHz EMF did not induce oxidative stress inwheat at investigated parameters. Further researches on additional parameters of oxidative stressare needed to estimate the possible oxidative effects of mobile phone radiation on plants.62

P.IV.8 ADAPTATIVE RESPONSE OF SHEWANELLAPUTREFACIENS AND PSEUDOMONAS AERUGINOSA TO TOXICORGANIC SOLVENTSM. M. LĂZĂROAIE** Center of Microbiology, Institute of Biology, 296 Spl.Independentei St, 060031,Bucharest, Romania, e-mail: mihaela.lazaroaie@ibiol.roOrganic solvents with the logarithm of the partition coefficient in n-octanol-water mixture(log P OW ) between 1 and 5 are compounds with high toxicity for the bacterial cells, and, asa result of their partition in lipid bilayer, they produce significant modifications in themembrane structure and functions. These modifications, together with the loss of lipidsand proteins, lead to irreversible damage, resulting in the death of the cell. Recently, agrowing number of bacterial strains able to overcome these toxic effects have been found.Using enrichment procedures, I isolated two bacterial strains from seawater, which aretolerant to organic solvents. Alkanes (i.e., n-hexane, n-heptane) with log P OW between 3.86and 4.39, were less toxic for Shewanella putrefaciens and Pseudomonas aeruginosa,compared with aromatics (i.e., toluene, styrene, xylene isomers, ethylbenzene,propylbenzene) with log P OW between 2.64 and 3.69. Cell tolerance, cells viability,adhesion and β-galactosidase activity of Shewanella putrefaciens and Pseudomonasaeruginosa in the presence of organic solvents (alkanes, aromatics) depends not only ontheir physicochemical properties, but also on the specific response of the cells, and thecellular response is not the same for these bacteria. The results indicated thatPseudomonas aeruginosa is more tolerant to organic solvents and other toxic compoundsthan Shewanella putrefaciens, although both bacterial strains posseshydrophobe/amphiphile efflux 1 transporter genes. There was observed the existence ofsome plasmids in Pseudomonas aeruginosa, while Shewanella putrefaciens do notharbored plasmid. The adaptation mechanisms (i.e., modification of cell hydrophobicity,changes in the membrane’s lipid and protein content), underlying solvent tolerance inShewanella putrefaciens and Pseudomonas aeruginosa showed a complex response ofbacterial cells to the presence of organic solvents in the culture medium.63

P.IV.10 COMPARISON BETWEEN CLASSICAL IDENTIFICATIONMETHODS FOR YEAST AND RAPID PHYSIOLOGICALTESTS API 20-CANA-MARIA NICOLAE, GETUTA NICOLAE,ELENA BRINDUSE*, AUREL CIUBUCA**, FLORENTINA MATEIUSAMV Bucharest, Faculty of Biotechnology* ICDVV Valea Calugareascam Prahova, Romania** SCDVV Bujoru, Galati, RomaniaKey words: wine yeast, isolation, identification, rapid physiological testsRomania is known as an important wine producer on the market and there is a trend inautochthonous wines. This wines should be obtain from local grape and by local starter cultures.Thiswork belongs to a national project and the main objectives are the study of the wine yeast biodiversityand to built a collection of yeast with oenological potentialIsolation and identification of mo from Moldavian wineriesThe biological material has consisted in grapes varieties for red (Feteasca neagra, Babeascaneagra, Cabernet Sauvignon, Merlot) and white (Feteasca alba, Feteasca regala, Aligote, Sauvignonblanc, Italian Riesling, Rkatiteli) wines as from Iasi and Dealu Bujorului counties. Also, themicroorganisms have been isolated from fermenting grape juice, from wines and from the storageplaceThe yeast isolation was performed on YEPD medium and incubated during 3 days/27 o C.Identification of the yeast: by morphological (Dalmau diagram) and physiological tests andby rapid test API 20 CThe isolation and identification results shows the following biodiversity of the yeast inMoldavia region:Saccharomyces cerevisiae ellipsoideus (28 strains-Dealu Bujorului, 19-Iasi);Saccharomyces oviformis (5 strains-Dealu Bujorului, 5-Iasi);Saccharomyces rosei (5 strains-Dealu Bujorului, 3-Iasi); Saccharomyces bayanus (3 strains-Dealu Bujorului, 3-Iasi);Saccharomycesacidifaciens (3 strains-Dealu Bujorului);Candida mycoderma (5 strains-Dealu Bujorului);Klöeckeraapiculata (3 strains-Dealu Bujorului);Rhodotorula mucilaginosa (2 strains-Dealu Bujorului)A total of 84 strains belonging to S. cerevisiae ellipsoideus (56%), S.oviformis (11,9%),S.rosei (9,52%), S.bayanus (7,14%), S.acidifaciens (3,57%), C.mycoderma (5,95%), Klöec.apiculata(3,57%), Rhod.mucilaginosa (2,38%) have been isolated in Iasi and Dealu Bujorului wine-makingcounties and some of them are under oenological characterization.These identified strains will beanalyzed by molecular tools.64

P.IV.11 CONSERVATION METHODS USED FOR YEASTISOLATED FROM VINEYARDS – LYOPHILISATIONADVANATGESGETUTA NICOLAE, ANA-MARIA NICOLAE, PETRE STELIAN*,FLORENTINA MATEI, STEFANA JURCOANE*USAMV Bucharest, Fcaulty of Biotechnology, Romania* Centre of Microbial Biotechnologi BiotechgenKey words: yeast conservation, freezed dried microorganism, lyophlisation protocolIn this draft protocol was established by freeze-preservation of 45 yeast strains isolated fromvineyards.Setting on the freeze drying method consisted in determining optimal safety environment formicroorganisms, optimal sampling phase that were to be freeze-dried, and working conditions offreeze drying device (pressure 0.04 mbar, temperatures between - 60 and – 70 0 C, for 6 hours).Yeast strains were subjected to freeze drying process, as follows: achievement subculture toobtain a pure culture of the genetic, environmental protective culture processing, distribution in vials,freezing of biological material, drying under vacuum, and finally closing the ampoules were placed inthe freeze drying device.For all lyophilised samples it have been determine their viability (from 30 to 55%).65

P.IV.12 GROWTH OF PSEUDOMONAS AERUGINOSA IN PILOTBIOREACTOR AND ITS TESTING IN THE BIOREMEDIATIONOF SOILS TREATED WITH PESTICIDES SUCH ASDINITROPHENOLSPETRE STELIAN MATEI, ANGHEL LAURA, GETUTA NICOLAE*,CAMELIA DIGUTA, STEFANA JURCOANEMicrobial Biotechnological Centre “BIOTEHGEN”, Blvd Marasti, No. 59, Bucharest, ROMANIA*University of Agricultural Sciences and Veterinary Medicine, Faculty of Biotechnology, StudentE-mail: matei_stelian2006@yahoo.comToxicity of some herbicides and insecticides to the environment is a global issue and onefactor is because in agriculture most of the farmers use as herbicides and insecticides manydinitrophenols and dinitrophenols derivates with a high toxicity for soils, plants and with side effectsfor humans.This agents can be degradated by some microorganism such as Pseudomonas aeruginosastrains without unwanted side effects for the environment.For that we growth Pseudomonas aeruginosa in a pilot bioreactor with minimal nutrientsfor 36 hours at continuously parameters such as pH, temperature, stirrer and air flow. The resultingbiomass was tested for the capacity of this bacteria in biodegradation of KARATHANE, adinitrophenol insecticide.We treated with KARATHANE un experimental field and after that we applied the bacteriaPseudomonas aeruginosa for biodegradation. The activity of this bacteria on the oils treated withpesticides was measured every 24 h for a period of 7 days.The efficiency of bioremediation are discussed.66

SECTION V: FOOD SAFETYP.V.1 ROMANIAN ECOLOGICAL FOOD MARKET: ANOVERVIEWMIHAELA DRĂGHICI, ALEXANDRA POPA, MONA POPA, PETRU NICULITA,GEICU MIHAELA, AMALIA MITELUTUniversity of Agronomic Sciences and Veterinary Medicine Bucharest, Romaniamdraghici@agral.usamv.roKey words: ecological product, food market, food ecological consumers.Ecological products consumption, in the present, is still low in Romania, making for only0.5 percent of the total food consumption. Many Romanians are now discovering ecologicalfoodstuffs, which are more expensive, but considerably healthier. Buyers of such products fall into thecategory of informed, concerned -about - their -health people, unlike the rest of the population thatprefers common, low-priced foodstuffs.The data supplied by the Agriculture Ministry revealed that last year there were 4,191ecological operators, 9.31 percent more than in 2007. With a total estimated value of 20 million Euroin 2009, Romania's ecological product market will see an increase of around 33 per cent compared to2008. However, those involved in this business segment have claimed that the sector is still facingdifficulties due to the poor information available to producers and consumers alike and the lack offinancial support from the authorities.This work is based on a market study made in the first quarter of year 2009, which has thepurpose to establish what kind of ecological products are available in the Romanian food market,what are the prices for ecological products, the country of origin and the availability of this productsin the supermarkets.67

P.V.2 COLOUR CHANGES IN MINCED PORK MEATINOCULATED WITH PSEUDOMONAS AERUGINOSA ANDTREATED BY OHMIC HEATINGMITELUŢ*, MIHAELA GEICU*, MONA POPA*, PETRU NICULITA*,RADU CRAMARIUC**, IOANA VĂTUIU***,DANIELA VĂTUIU***, MIRCEA POPESCU*****University of Agronomic Sciences and Veterinary Medicine Bucharest, Romania**Electro-technologies Reasearch Center, Bucharest, Romania***S.C. ICA R&D S.R.L. Bucharest, Romania****Politehnica University Bucharest, RomaniaKey words: colour measurement, minced pork meat, ohmic heating, Pseudomonas aeruginosa.The color of meat is critically appraised by consumers and often is their basis for productselection or rejection. The current study compared the colour of fresh minced pork meat with thecolour of meat inoculated with Pseudomonas aeruginosa after 24 respectively 48 hours from theinoculation moment. Meat colour was measured with a Hunter Lab spectrocolorimeter. CIE Labvalues, also known as L*, a*, b* colour indicators or tristimulus values, and reflectance at specificwavelengths, between 400 -700 nm, have been used to express color data obtained.In this study it was also observed the colour changes in P. aeruginosa inoculated mincedpork meat after 10 minutes of ohmic treatment at 81 o C. Ohmic heating is an advanced thermalprocessing method wherein the food material, which serves as an electrical resistor, is heated bypassing electricity through it. Electrical energy is dissipated into heat, which results in rapid anduniform heating. Ohmic heating is also called electrical resistance heating, Joule heating, or electroheating,and may be used for a variety of applications in the food industry.68

P.V.3 ELECTRONIC NOSE FOR DISCRIMINATION OFROMANIAN APPLESELENA M. PRUTEANU 1 , DENISA E. DUTA 1 ,FULVIA A. HINCU 1 , MIRELA CALU 21 Institute of Food Bioresources, pruteanu_elena_marilena@yahoo.com, denisa_duta@yahoo.com,hfulvia@yahoo.com2 “Dunarea de Jos” University of Galati, Faculty of Food Science and Engineering, mirelacalu@yahoo.comKey words: E-nose, apple, multivariate statistics (PCA, DFA, SQC)An electronic nose (E-nose) was used to classify apple samples based on their smell. A total of sevenvarieties (Golden, Starkrimson, Jonathan, Pinova, Pioneer, Fuji, Gala) of apples from Romania(September 2009 production) were used. Some of the apple samples were from Reghin region, somefrom Insuratei area and other were bought from a supermaket in Bucharest. All the samples wereanalyzed using the E-nose FOX 4000 with 18 metal oxide coated or uncoated sensors. The resulting E-nose intensities were analyzed by Principal Component Analysis (PCA), Discriminant Factor Analysis(DFA) and Statistical Quality Control (SQC), which resulted in grouping the used varieties of applesor in grouping the types of samples (peel, homogenate or diluted homogenate from the same apple).The obtained results indicated that E-nose could discriminate successfully among varieties of apples(% of variance >> 90; percentage of recognition ≈ 100 %).69

SECTION VI: MISCELLANEOUSP.VI.1 IMPROVED PRODUCTION OF BACTERIAL CELLULOSEBY ACETOBACTER XYLINUM USING DIFFERENT CARBONSOURCESANGELA CASARICA*, CORINA IONESCU, MARIANA SOARE, ELEONORAGHEORGHIU, MISU MOSCOVICI, ALEXANDRA GHIORGHITA, VASILICAZAHARACHESCU, ALINA DOBRE, ILEANA IONICANational Institute for Chemical Pharmaceutical Research and Development, 112 Vitan Ave, 031299Bucharest, RomaniaCorresponding author : anngissa_30@yahoo.com, Tel : +40213222916Key words: bacterial cellulose, Acetobacter xylinum , cellulose membrane, waste fruit, carbonsource.In this paper, we present a method of bacterial cellulose biosynthesis by Acetobacterxylinum using different carbon and nitrogen sources.The optimal cultural requirements were a pH between 5 – 5.5, temperature at 28°C withammonium salts as nitrogen source and either glucose p.a. or glucose from fruit wastes (apple) ascarbon source.Production rate was also improved by adding ethanol 1,4% (v / v).The production rate of cellulose on flasks was between 1.12-8g/L.70

P.VI.2 MODERN STUDIES FOR OBTAINING VOLATILES OILSFROM MEDICINAL AND AROMATIC PLANTS IN ORDER TOREALIZE THE THERAPEUTIC BIOPREPARATESMARIA ICHIM * , RAMONA ENACHE * , IULIA ADRIANA GRAFU * ,ADRIANA VISAN * , ALICE ARMATU *** Institute of Biotechnology and Environmental Protection - S.C. BIOING S.A., Bucharest, Romania** National Institute for Chemical and Pharmaceutical Research and Development - Bucharest, RomaniaKey words: volatiles oils, bioproducts, extraction, supercritical fluids.In this study is presented the possibility of obtaining some bioprepareds based on volatilesoils, with utilization in therapy of diseases with microbial origin and in symptomatic treatment ofdigestive disorders.The vegetable material is represented by medicinal and aromatic plants with a high content ofvolatiles oils, material anterior selected.From the experiments was been dignified the chemical composition of volatiles oils; Carumcarvi L. - 50-65% carvone, 30-45 % limonene; Achillea millefolium L. - 30% thymol and paracymene,30-40% carvacrol; Thymus vulgaris L. 36 - 55% thymol; Salvia officinalis L. 18-43% A-thujone, 3 - 9% β-thujone, 6 - 13% 18-cineole, 3-9% camphor; Fooeniculum vulgare Mill. 55-75%(E) - anethole, 12-26% fenchone, 1-5% limonene.For obtaining of volatiles oils was elaborated an experimental model of extraction withsupercritical fluids.P.VI.3 STUDIES CONCERNING THE PREPARATION AND THECHARACTERIZATION OF NAFTIFINE LOADED LIPOSOMESCRISTINA HLEVCA, ELENA PATRUT, MINERVA PANTELI,RASHIT YUKSEL, RODICA GUTAInstitutul National de Cercetare-Dezvoltare Chimico-Farmaceutica ICCF BucurestiNaftifine is a broad spectrum antifungal agent of allylamine derivative group which has shown highefficacy as a topical agent against various types of dermatomycoses.The entrapment of a drug inliposomes can facilitate localized delivery of the drug and improved availability by means of acontrolled release pattern.The experimental studies investigated the variation of the characteristics of the liposomes(entrapment efficiency and stability) depending on the formulation parameters (weight ratio ofphospholipids, cholesterol and naftifine.). It was demonstrated that by modifying the formulation,liposomes with entrapment efficiencies over 80% can be obtained.71

Based on the in vivo studies, it was concluded that significantly higher retention of naftifine in theskin from liposomes has been achieved as compared to a solution containing naftifineP.VI.4 THE INFLUENCE OF EXTRACTION TECHNIQUES ONRADICAL SCAVENGING ACTIVITY OF Polygonum aviculare L.EXTRACTSGEORGETA NEAGU*, CORNELIA NICHITA*, VIRGINIA VULTURESCU*,GEORGETA RADULESCU** National Institute for Chemical-Pharmaceutical Research and Development, Bucharest, Romania,e-mail: getabios@yahoo.comKey words: Poliygonum aviculare L., DPPH, ultrasonic and microwave-assisted extractionsIt is well known that free radicals are the principal cause of several diseases and phenolicsplay a preventive role in the development of cancer, heart disease and ageing-related diseases.In this study the effects of extraction techniques on the antioxidant activities of some vegetalfractions obtained from Polygonum aviculare L. were investigated. The applied extraction techniqueswere: classical, ultrasonic and microwave-assisted extractions. The antioxidant measurementsincluded 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging activity and chemiluminescencemethod.The results of the DPPH and chemiluminescence assays shows the highest antioxidantactivities for the ultrasonic-assisted extraction. Also, the fractions obtained through microwaveassistedextraction exhibit high values of free radical scavenging effect.Antioxidant activity was correlated with the polyphenolic content and the extractiontechnique, and was attributed to the complexity of the plant extracts. Also, the results demonstratedthat Polygonum aviculare L. has high phenolics content with excellent antioxidant activity.72

P.VI.5 OLIGOPROANTHOCYANIDINS INHIBITSTHE GENOTOXICITY OF CICLOPHOSPHAMIDE in vivoGEORGETA NEAGU*, VIRGINIA VULTURESCU*,CORNELIA NICHITA*, BEATRICE BURGHELEA**, RADU ALBULESCU** National Institute for Chemical-Pharmaceutical Research and Development, Bucharest, Romania,e-mail: getabios@yahoo.com** The National Institute of Research and Development for Microbiology and ImmunologyCantacuzino”, Bucharest, RomaniaKey words: oligoproanthocyanidins, antimutagenic, grape seed and skins extractMany byproducts and residues generated by the agroindustries contain polyphenols withpotential application as preventive agents against cancer and cardiovascular diseases. Among thesepolyphenols, oligomeric proanthocyanidins (OPCs) are particularly significant. Grape seed and skinsproanthocyanidins have been reported to possess a broad spectrum of pharmacological andmedicinal properties.In the present study we have evaluated the antimutagenic potential of a grape seed and skinsextract (GSE) containing OPCs against ciclophosphamide (50 mg/kg) induced genotoxicity in Swissalbino mice. The tested GSE was selected among 27 GSEs, after they were analyzed by HPLC andtheir radical scavenging activity was determined by the DPPH method. The effect of GSE was studiedby in vivo micronucleus test. The doses of GSE (25 and 50 mg/kg) were given orally for 5 days priorto the administration of ciclophosphamide (CP). The micronucleated polychromatic erythrocytesfrecvency at 24h and 48h after administration of CP was significantly lower in GSE-pretreated mousebone marrow than those observed in the group treated with CP only.The results indicate that the constituents of the GSE possess antigenotoxic properties in termsof micronucleus formation.73

P.VI.6 SUCCESSFULLY APPLICATION OF INNOVATION INTRADITIONAL TECHNOLOGIESE. MARCU 1 , G. NEDITA 21University of Agricultural Sciences and Veterinary Medicines, Bucharest, Romania2Institute of Food Bioresources, Romania, neditag@yahoo.comThe article presents the results of a 6 months study, performed in an outstanding smallcheese company, including animal production and processing plant.The purpose was to make a comparative analysis about the influence of quality of rawmaterial on quality of final products. A battery of tests (phisico-chemical and microbiological) havebeen done for sheep milk. The study was developed in 2008, during the lactation season (betweenApril and September) of animals. Raw milk was provided by own herd of company (Turma 1) andindividual farmers.The company is S.C. ASI NATURE S.R.L., from Tichindeal village, Sibiu county,Transilvania region. In this small plant is made the product named sheep chees «Harbach», a kind ofTelemea. Both raw milk and final products are certificated as ecological. Final product is attestedalso as traditional product by Ministry of Agriculuture.Gathered data are important for these kind of studies developed in rural areas, izolatedfrom geopgraphical perspective and it could be a valuable input for decision makers. This study is asuccesful example about how innovation support the high quality of products keeping in the sametime the specific test of these products.74

P.VI.7 KINETICAL CHARACTERIZATION OF LIPOSOMES WITHANETHI AETHEROLEUMALINA ORTAN 1 , CRISTINA DINU PIRVU 21Universitatea de Stiinte Agronomice si Medicina Veterinara – Facultatea de Biotehnologii –Bucuresti2 Universitatea de Medicina si Farmacie Carol Davila - Facultatea de Farmacie – Chimie fizica,coloidala si Biofarmacie, str. Traian Vuia, nr.6, S2– BucurestiAn essential stage in characterization of liposomal systems as drug vectors is the analysis ofthe entrapped substances release rates. In vitro release studies are used not only for thecharacterization of different therapeutically formulations, but also for forecasting the in vivobehavior. The proposed liposomal formulations have been tested in order to establish the factorsinfluencing the release rate of volatile oil of Anethum graveolens. The liposomes were prepared usingthe thin lipidic films hydration method, and the evaluation of the release kinetics was performed bydialysis method, with an Essadisolver instrument. The medium was an hydroalcoholic phosphatebuffer (with pH = 7.4) : ethanol, ratio 3 : 1. The experiments were performed for 0 – 48 h interval.The experimental data were processed in order to verify different kinetical models. Weconcluded that, for a 24 h period, the release from liposomes respects 0 order, so they can constitutecontrolled release system of Anethi aetheroleum. All the studied formulations indicated an increase inthe release rate with the increase of the phosphatidyl coline quantity. The same trend is followed bythe released active substance percentages at 24, respectively 48 h. In contradistinction to the directproportionality between the value of the release rate and the quantity of the volatile oil, respectivelyphosphatidyl coline, in case of the increase of the cholesterol quantity in liposomal formulations adecrease of release rate can be noticed.The in vitro release studies demonstrated that the liposomes are reservoir systems, allowing arelatively constant release of Anethi aetheroleum and therefore a long term effect.75

P.VI.8 THE EFFECT OF FORMULATION PARAMETERS ON THECHARACTERISTICS OF SOME TRANSFEROSOMES WITHSTEROID HORMONESCRISTINA DINU PIRVU 1 , CRISTINA HLEVCA 2 , ALINA ORTAN 3 ,ELENA PATRUT 2 , LUIGI SILVESTRO 41 Universitatea de Medicina si Farmacie Carol Davila - Facultatea de Farmacie – Chimie fizica,coloidala si Biofarmacie, str. Traian Vuia, nr.6, S2– Bucuresti2Institutul National de Cercetari Chimico Farmaceutice – ICCF- Bucuresti3Universitatea de Stiinte Agronomice si Medicina Veterinara – Facultatea de Biotehnologii –Bucuresti4 SC Pharma Serv SRL - BucurestiIn the last years, the researches in the pharmaceutical field are pointed toward the use ofminimal quantity of active substances, which can provide maximum therapeutic effect on the desiredduration, with minimal side effects and a good compliance for the patient. The vesicles have becomepreferred drug vectors for controlled release of active substances, because of their advantages (theycan increase the circulation time by incorporating the drug in liposomes, they are biodegradable andwith low toxicity, they can incorporate both hydrophilic and lipophilic molecules, they can provide acontrolled release of the drugs, the can increase the bioavailability of the active substances).The studied elastic vesicles are starting point in designing a transdermal system, which canprovide a controlled or even targeted release of the entrapped hormones. Transferosomes wereprepared using the lipidic film hydration method. The effects of different formulations on thecharacteristics of vesicles have been investigated (entrapments efficiency, size distribution andstability of obtained population).In case of the proposed transferosomes formulations we concluded that the entrapment ofthe steroid hormones has a good percentage, the increase of the sodium cholate concentrationconducts to an increase in the entrapment efficiency and the stability is significantly influenced by thepreservation parameters, especially temperature (at a 37 0 C temperature they lose approximately 25%from the entrapped substance in1 month).76

P.VI.9 FEEDBACK MECHANISMS CONNECTED TOCLIMATE CHANGE DYNAMICSR. PASCUKey words: Albedo effect, anthropogenic activity, GHG emission, solar radiation, thermalequilibriumFeedback is a widespread concept, so that it is familiar in fields like: electronics, economy, biology,etc. It is easier to understand the complex process of climate change using this concept because of thevisual representation through diagrams. Given a natural process connected to climate change, onecan better explain its dynamics as a negative or positive feedback and can also find ways to stop itsacceleration. The paper deals with some of the most comprehensible ideas drawn out from the modelof climate feedback dynamics in order to be used for educational purposes. The model was built up inthe frame of Meridian Programme directed by David Wasdell and is based on the thermodynamics ofthe planet as a whole, in its context with the Solar System. It raises issues of urgent nature for theworld community and calls in question the effectiveness of current strategic responses to globalwarming.P.VI.10 THE INFLUENCE OF CHEMICAL AND ORGANICFERTILIZATION ON WHEAT QUALITYMARIA M. SANDRIC 1 , CLAUDIA E. MOSOIU 1 , V. SIMAC 11 - Institute of Food Bioresources, 6 Dinu Vintila street, 2 nd district , Bucharest, RomaniaKey words: Wheat, Fertilization, Quality, OrganicThe aim of this study is to test the influence of crop rotation and fertilization with differentdoses of nitrogen and potassium, on physical-chemical quality of wheat production, such as proteinand gluten content, hectolitric weight, moisture, deformation index and gluten index, so on. Rawmaterials were represented by twenty samples of Dropia wheat, using different experimental variants.So, as preliminary plants were used wheat, maize, pea, sun-flower, and for fertilization there wereused the following combinations: N90P0, N0P75, N90P75. Also, it was obtained organic wheat usingno fertilizers and fertilization with manure. Finally, it was choosen the most convenient crop rotationand fertilization.77

P.VI.11 BACTERIAL CELLULOSE MEMBRANES CONTAININGSILVER PARTICLES USED AS ANTIBACTERIAL MATERIALSA. STOICA, L. M. DRAGNE, S. JINGA, M. STROESCU,I. JIPA, C. JINGAUniversity Politehnica of Bucharest, RomaniaKey words: Bacterial cellulose, silver nanoparticles, antibacterial activityBacterial cellulose (BC) is an interesting biomaterial produced by Acetobacter xylinium.This material can be used for medical applications as temporary substitute for human skin, or forobtaining artificial blood vessels for microsurgery. But, itself BC has no antimicrobial activity. Toachieve devices containg bacterial cellulose with antimicrobial activity, impregnation of BCmembranes with silver nanoparticles seems to be among the most popular way.The aim of this work is to present an experimental study for obtaining bacterial cellulosemembranes impregnated with silver particles, using Tollens reaction. The obtained membranes werecharacterized using scanning electron microscopy and X-ray diffraction. Antimicrobial activity ofsilver nanoparticle-bacterial cellulose membrane system was tested against different microorganisms.78

P.VI.12 ELECTRONOGRAPHIC HIGHLIGHTING OF THELUMINISCENCE OF SOME STRUCTURES FROM NATURERADIANA TAMBA-BEREHOIU*, CIPRIAN POPA**,STELA POPESCU*, RODICA CULEA** University of Agricultural Sciences and Veterinary Medicine Bucharest, Faculty ofBiotechnology** S.C. Farinsan S.A. GiurgiuKey words: electronography, strimers, periproximal, pelicular and electromorphic effectsThe electronographic technique is used for highlighting the electromagnetic fields of theliving and non-living structures. These fields exceed the dimensions of their physical structures andexpress themselves through luminescence. The luminescence is correlated with the living systems’metabolism (bioluminescence) and with the structural peculiarities of non-living bodies.Luminescence and bioluminescence highlight the periproximal effects (effects of the environmentsurrounding the structure), the pelicular effects (at the surface of the structure), and electromorphiceffects (in the volume of the structure).We tested by the electronographic technique (we applied a short electric impulse, havinghigh frequency and high voltage) the following structures: seeds, leaves, human fingers and palms,drops of liquid. The luminiscent fingerprints, called streamers, issued by bodies taken in the study,were photographed and computer analyzed.The particularities of the photographed luminiscence correlated with the health andintegrity of seeds, with their power of germination, with the level of nutrition and the existence ofsome lesions, scars or unpigmentation for human subjects and with the ionic load or the chemicalcomposition of the drops of liquid.In conclusion, electronography is an innovative and effective method for establishing rapiddiagnostics, in particular related to subtle changes, otherwise imperceptible, on the metabolic andphysiological aspects, on the ionic composition, on the level of communication of the structure withthe surrounding environment, on finding external aggressions etc, which affect the electromagneticfields of the bodies in nature. We consider that the electronographic method finds a wide researchfield in biotechnologies.79

P.VI.13 THE DETERMINATION OF BEST MARKETINGSTRATEGY BY THE PRODUCERS BASED ON CONSUMERS’BEHAVIOR DURING THE ECONOMIC CRISISU.S.A.M.V. BucharestMARGARIT GABRIELA 12 , TOMA R.C.¹, STOENOIU CRISTINA¹Key words: e-Commerce; internet; websites; marketing strategies; companies; cross–bordertransactions, consumersAs major financial institutions crumble and banks lending less, businesses are starting to feel theheat. With smaller budgets and lighter wallets, consumer spending also decreased. Pushing throughthese troubled times means cutting costs without being too conservative as well as adapting themarketing strategies based on consumers’ behavior during economic crisis.Businesses that rely heavily on TV, print, or mail suffer heavily from the budget costs. However,websites that have already established a strong internet presence can now sit back and reap thebenefits of their marketing investments over the last few years.A key medium for consumer purchasing is electronic commerce, since it has an unrivalled potentialfor cross-border transactions, and thus providing consumers with the opportunity to benefit fully fromopen competition across the internal market.E-Commerce is one of the most important facets of the Internet to have emerged in the recent times.E-Commerce or electronic commerce involves carrying out business over the Internet with theassistance of computers, which are linked to each other forming a network.The direct cost-of-sale for an order taken from a web site is lower than through traditional means(retail, paper based), as there is no human interaction during the on-line electronic purchase orderprocess. Also, electronic selling virtually eliminates processing errors, as well as being faster andmore convenient for the visitor.Operational benefits of e-Commerce include reducing both the time and personnel required tocomplete business processes, and reducing strain on other resources. It’s because of all theseadvantages that one can harness the power of e-Commerce and convert a business to e-business byusing powerful turnkey e-Commerce solutions made available by e-business solution providers.80

822 nd International Symposium on New Research in Biotechnology19-20 th November 2009, Bucharest, RomaniaParticipants listNo. Participant Affiliation Presentationcode1. ANGHEL LAURA CBM Biotehgen, Bucharest, Romania P.IV.12. ARDELEAN ION Institute of Biology of the RomanianAcademy, Center of Microbiology, Bucuresti,P.IV.23. BADARAU CARMENLILIANARomaniaNational Institute of Research andDevelopment for Potato and Sugar BeetBraşov, RomaniaP.IV.3O.I.2O.I.44. BALAN DANIELA USAMV Bucharest, Faculty of Biotechnology, P.IV.4Bucharest, Romania5. BANJANAC TIJANA Institute for Biological Research “Sinisa P.IV.5Stankovic”, Belgrade, Serbia6. BĂLĂNUȚA ANATOL Technical University of Moldova, Chisinau P.III.17. BORDA DANIELA Faculty of Food Science and Engineering O.III.1University Dunarea de Jos, Galati, Romania8. CASARICA ANGELA National Institute for ChemicalPharmaceutical Research and Development,Bucharest, RomaniaP.VI.19. COGALNICEANU GINA Institut of Biology, Bucharest, Romania P.I.110. CORNEA PETRUTA USAMV Bucharest, Faculty of Biotechnology O.I.511. CRISTEA VICTORIA “Babes-Bolyai” University, “AlexandruBorza” Botanical Garden, Cluj-Napoca,RomaniaO.I.112. CULEA RODICA ELENA USAMV Bucharest, Faculty of Management,Economic Engineering in Agriculture andRural Development, Bucharest, Romania13. DANAILA-GUIDEASILVANA M.USAMV Bucharest, Faculty of Biotechnology,Laboratory of Plant Biotechnology, Bucharest,Romania14. DIMITROVA MILENA Department of Plant PhysiologyBiological Faculty Sofia University, Bulgaria15. DRAGHICI (TUDORIE) USAMV Bucharest, Faculty of Biotechnology,MIHAELABucharest, Romania16. EANCHE RAMONA Institute of Biotechnology and EnvironmentalProtection - S.C. BIOING S.A., Bucharest,Romania17. GEICU MIHAELA USAMV Bucharest, Faculty of BiotechnologyBucharest, Romania18. GHERGHINA EVELINA USAMV Bucharest, Faculty of BiotechnologyBucharest, Romania19. HLEVCA CRISTINA ICCF BucharestRomania20. HOLOBIUC MIHAELA IRINA Institute of Biology, Romanian Academy,Bucharest, Romania21. IONESCU ANA DESPINA National Chemical-Pharmaceutichal forResearch and Development Institute,Bucharest, Romania22. ISRAEL-ROMINGFLORENTINAUSAMV Bucharest, Faculty of BiotechnologyBucharest, RomaniaP.I.2P.I.3P.IV.6P.V.1P.VI.2P.III.3P.I.4P.VI.3P.I.5P.III.4O.V.6

23. JURCOANE STEFANA USAMV Bucharest, Faculty of BiotechnologyBucharest, Romania24. KAPCHINA-TOTEVAVENETADepartment of Plant Physiology, Faculty ofBiology, St. Kl. Ohridski Sofia University,Bulgaria25. KIROVSKI DANIJELA Faculty of Veterinary Medicine, University ofBelgrade, Belgrade, Serbia26. KOUZMANOVADepartment of Plant PhysiologyMARGARITABiological Faculty Sofia University, Bulgaria27. LAZAROAIE MIHAELA Institute of Biology of the RomanianMARILENAAcademy, Center of MicrobiologyBucharest, Romania28. LEGRAS JEAN-LUC UMR 1131 – SVQV équipe MSV - INRAUniversité de Strasbourg, France29. LUTA GABRIELA USAMV Bucharest, Faculty of BiotechnologyBucharest, Romania30. MARGARIT GABRIELA USAMV Bucharest, Faculty of BiotechnologyBucharest, Romania31. MARINESCU CLAUDIA National Institute for ChemicalPharmaceutical R&D, Bucharest, Romania32. MARINESCU GINA Faculty of Food Science and Engineering,Galati, Romania33. MATEI FLORENTINA USAMV Bucharest, Faculty of BiotechnologyBucharest, RomaniaP.IV.1;P.IV.11;P.IV.12P.I.6P.I.7O.II.1P.IV.7P.IV.8O.III.2P.I.8P.III.5P.IV.9P.III.6O.IV.4P.IV.10P.IV.1134. MIHOVA TEODORA RIMSA – Troyan, Bulgaria P.I.935. MISIC DANIJELA Institute for Biological Research”Siniša P.I.19Stanković”, Belgrade, Serbia36. MITELUT AMALIA USAMV Bucharest, Faculty of Biotechnology P.III.7Bucharest, Romania37. MOLDOVAN LAURENTIU SC RODAX IMPEX SRBucharest, RomaniaO.V.338. NEAGU GEORGETA INCDCF- ICCF BucharestRomania39. NEDITA GABRIELA Institutul de Bioresurse Alimentare, Bucharest,Romania40. NICHITA CORNELIA INCDCF- ICCF BucharestRomania41. NICOLAE ANAMARIA USAMV Bucharest, Faculty of Biotechnology,Bucharest, Romania (college student)42. NICOLAE GETUTA USAMV Bucharest, Faculty of Biotechnology,Bucharest, Romania (college student)43. NICULITA PETRU USAMV Bucharest, Faculty of Biotechnology,Bucharest, Romania44. NISTOR (TICAN) ANDREEA National Institute of Research andDevelopment for Potato and Sugar BeetBrasov, ROMANIA45. ORTAN ALINA USAMV Bucharest, Faculty of BiotechnologyBucharest, Romania46. PANAGOPOULOUGHIDURUS MIHAELAUSAMV Bucharest, Faculty of BiotechnologyBucharest, RomaniaP.VI.4P.VI.5P.VI.6P.III.8P.III.9P.IV.10P.IV.11O.V.4P.I.3; P.III.2;P.III.8P.V.1P.I.10P.I.11P.VI.7P.VI.8O.V.583

ELENA47. PANTEA GABRIELA SC RODAX IMPEX SRBucharest, Romania48. PASCAL SVETLANA LIVIA Faculty of Food Science and Engineering,Department of Biochemistry, Galati, Romania49. PASCU RALUCA USAMV BucurestiBucharest, Romania50. PATRASCU LIVIA (CERCEL Faculty of Food Science and Engineering,FLORICEL)Galati, RomaniaO.V.4O.IV.1O.IV.2P.VI.9P.III.108451. PETRE MARIAN University of Pitesti,Faculty of SciencesRomaniaO.IV.3O.IV.552. PETRE STELIAN MATEI CBM Biotehgen BucharestP.IV.12Romania53. PODOSU AURELIA Statiunea de Cercetare-Dezvoltare pentru P.I.12Viticultura si Vinificatie Odobesti, Vrancea54. POPA ALEXANDRA USAMV Bucharest, Faculty of Biotechnology O.III.3Bucharest, Romania55. POPA GABRIELA USAMV Bucharest, Faculty of Biotechnology O.I.5Bucharest, Romania56. POPA MONA ELENA USAMV Bucharest, Faculty of Biotechnology P.III.1157. PRUTEANU ELENAMARILENAInstitute of Food BioresourcesBucharestRomania58. RADULESCU GEORGETA National Institute for Chemical-Pharmaceutical Research and DevelopmentBucharestRomania59. RAVICHANDRAN KAVITHA Institute of Food Technology and FoodChemistry, Berlin University of Technology,Germany60. RAYKOV SVILEN SHUMEN UNIVERSITY "EP. K.PRESLAVSKI"Bulgaria61. ROSU ANA USAMV Bucharest, Faculty of BiotechnologyBucharest, Romania62. SANDRIC MARIAINSTITUTE OF FOOD BIORESOURCES,MAGDALENABucharestRomania63. SILER BRANISLAV Institute for Biological Research “SinišaStanković”, Belgrade, Serbia64. STANILOVA MARINA Institute of Botany, Bulgarian Academy ofSciences, Bulgaria65. STERIAN ADRIANA USAMV Bucharest, Faculty of Biotechnology,Bucharest, Romania (master student)66. STOICA ANICUTA University Politehnica of Bucharest, Facultyof Applied Chemistry and Materials ScienceBucharest, Romania67. TAMBA-BEREHOIUUSAMV Bucharest, Faculty of BiotechnologyRADIANABucharest, Romania68. TOMA RADU CRISTIAN USAMV Bucharest, Faculty of BiotechnologyBucharest, RomaniaO.VI.1P.V.3P.III.12P.III.13P.I.13P.I.14-P.VI.10P.I.15O.I.3O.V.1P.VI.11P.VI.12P.VI.1369. TO THI MAI HUONG IUVV Jules Guyot, University of Burgundy, P.III.14

France70. ZAMFIR MEDANA Institute of Biology, Department ofP.III.15Microbiology, BucharestRomania71. ZAGREAN VALENTIN USAMV BucharestP.I.16Bucharest, Romania72. TURTOI MIRA USAMV Bucharest, Faculty of Biotechnology O.VBucharest, Romania73. VARGA MIOARA USAMV Bucharest, Faculty of Biotechnology P.VI.14Bucharest, Romania74. VISAN LUMINITA USAMV Bucharest, Faculty of Biotechnology P.VI.15Bucharest, Romania75. VATUIU IOANA CCAI Bucharest, Romania P.III.776. VULTURESCU VIRGINIA INCDCF- ICCF BucharestRomania77. WAWROSCH CHRISTOPH University of Vienna, Department ofPharmacognosy, Austria78. YORDANOVA ZHENYA Department of Plant Physiology, Faculty ofBiology, St. Kl. Ohridski Sofia University,BulgariaP.III.16-P.I.17P.I.1885