Abstract book 2011 - ISHAM

THE SPECTRUM OF SCEDOSPORIUM ISOLATES FROM THERESPIRATORY TRACT IN CF-PATIENTS – MULTI LOCUS SEQUENCETYPING (MLST) AND DOCUMENTED CLINICAL RELEVANCEAnne Bernhardt 1 , Ludwig Sedlacek 2 , Carsten Schwarz 3 , Benjamin Würstl 4 , SonjaWagner 5 and Kathrin Tintelnot 11 Robert Koch-Intitut, FG 16 Mykologie, Berlin, Germany2 Medizinische Hochschule Hannover, Inst. f. Med. Mikrobiologie und Hygiene, Germany3 Christiane Herzog Zentrum/Mukoviszidose, Charité-Universitätsmedizin, Berlin, Germany4 Max v. Pettenkofer-Institut, Universität München, Germany5 Helios Kliniken - Emil v. Behring Krankenhaus, Berlin, GermanyWithin the German network for Scedosporium in cystic fibrosis 158 Scedosporium isolatesfrom 51 patients have been collected and re-identified between 12/2008 and 11/2010. S.apiospermum was found in 53%, P. boydii in 22%, S. prolificans in 18% and S. aurantiacumin 7%. Although many patients are colonized for months or even years without significantclinical relevance, a dramatic decrease of the lung function was documented in patients inassociation with the isolation of S. apiospermum from the airways.MLST was performed for >110 P. boydii- and S. apiospermum isolates, including severalretrospective isolates of the same patient.The MLST based on identification of the species by sequencing of the ITS regions, and thefive loci actin (ACT), calmodulin (CAL), RNA polymerase II second largest subunit (RPB2),beta-tubulin (ßTUB) and Mn-superoxide dismutase (SOD2).The genotyping results revealed, that the individual patients can be colonized with anindividual genotype for years, rarely harbouring a second clone or species.