Abstract book 2011 - ISHAM

More documents

Recommendations

Info

originating from the same state. Strains did also not cluster according to their sources, i.e fromclinical specimen, veterinary or environmental origin. The high diversity among theAustralian strains suggests that S. aurantiacum may have originated within the Australiancontinent and subsequently dispersed to other regions, a fact revealed by the closephlyogenetic relationships between some of the Australian sequence types and those found inother parts of the world. The MLST data are accessible at mlst.mycologylab.org.
ROLE OF PTX3 IN CYSTIC FIBROSIS-ASSOCIATED INFECTIONSYveline Hamon 1,2 , Christine Person 3 , Jean-Louis Giniès 3 , Jean-PhilippeBouchara 4,5 and Yves Delneste 1,21 Inserm U892, Institut de Biologie en Santé-IRIS, Angers, France2 Université d’Angers, Angers, France3 CF Center, Centre Hospitalier Universitaire, Angers, France4 Groupe d'Etude des Interactions Hôte-Pathogène, UPRES-EA 3142, Université d'Angers,Institut de Biologie en Santé-IRIS, Angers, France5 Laboratoire de Parasitologie-Mycologie, Institut de Biologie en Santé-CHU, CentreHospitalier Universitaire, Angers, FrancePTX3, a soluble innate immunity receptor, binds to selected microbes and facilitates theirclearance by phagocytes. PTX3 selectively binds to Pseudomonas aeruginosa and Aspergillusfumigatus, two microorganisms frequently colonizing the airways of patients with cysticfibrosis (CF), and sometimes causing true respiratory infections. PTX3 -/- mice are sensitive toA. fumigatus infection, highlighting the role of this protein in the protection against thispathogen. We thus hypothesized that PTX3 could be altered in CF patients and that this couldbe responsible, at least in part, to their susceptibility to some opportunistic pathogens.Serum and sputum samples from 30 CF patients (20 adults and 15 children) and 7 patientswith chronic obstructive pulmonary disease (COPD) as the control group were analyzed forPTX3 expression and integrity by ELISA and Western-blotting, respectively. The role ofendogenous or microbial proteases on recombinant human PTX3 was also analyzed.Results showed that PTX3 level was increased in CF and COPD serum, highlighting theirinfectious/inflammatory status, while, in contrast, PTX3 concentration was lower orundetectable in CF sputum than in COPD. Western-blotting showed that PTX3 is degraded insputum samples from most of CF patients, but not in clinical specimens from COPD patients.The degradation of PTX3 was shown to be mediated by serine proteases. More precisely, boththe neutrophil elastase and the alkaline proteinase from A. fumigatus have the ability todegrade in vitro PTX3.This study which shows that PTX3 is degraded in respiratory secretions from CF patients,provide new insights into the pathogenesis of microbial colonization of the airways andrespiratory infections in CF patients, since degradation of PTX3 could be responsible, at leastin part, for the sensitivity of CF patients to some opportunistic infections.
Page 1 and 2: Second Meetingof the ECMM/ISHAM Wor
Page 3 and 4: Program___________Thursday Septembe
Page 5 and 6: 9 h 40 Role of real-time PCR and ga
Page 8 and 9: FUNGAL INFECTION/CONTAMINATION IN T
Page 10 and 11: ISOLATION OF FILAMENTOUS FUNGI IN T
Page 12 and 13: FILAMENTOUS FUNGI IN CF - PREVALENC
Page 14 and 15: Results and discussion: 300 patient
Page 16 and 17: PNEUMOCYSTIS JIROVECII AND CYSTIC F
Page 18 and 19: TRICHOSPORON MYCOTOXINIVORANS: AN E
Page 20 and 21: species Exophiala phaerumiformis (i
Page 22 and 23: SHORT AND LONG-TERM OUTCOMES WITH P
Page 24 and 25: ROUTINE MOULDS’ IDENTIFICATION IN
Page 26 and 27: SCEDOSPORIUM SPP.: DETECTION, IDENT
Page 28 and 29: ROLE OF REAL TIME PCR AND GALACTOMA
Page 30 and 31: MONOCLONAL ANTIBODIES SPECIFIC TO S
Page 32 and 33: REAL TIME TYPING OF ASPERGILLUS FUM
Page 34 and 35: THE SPECTRUM OF SCEDOSPORIUM ISOLAT
Page 38 and 39: ASPERGILLUS-INDUCED INFLAMMATION IN
Page 40 and 41: ECOLOGY OF THE AIRWAY MICROBIOME IN
Page 42 and 43: Discussion and conclusion: For the
Page 44 and 45: At concentrations >2 mg/L, AMB achi
Page 46 and 47: CLINICAL AND MICROBIOLOGICAL EFFICA
Page 48 and 49: Conclusions: Scedosporium spp. colo
Page 50 and 51: Conclusion: Defining the optimal me
Page 52 and 53: ATCF (AZOLE THERAPY IN CYSTIC FIBRO
Page 54 and 55: * For those using agar plates, spec
Page 56 and 57: ATTENDANTS
Page 58 and 59: * Pr. Josep F. Cano (Unitat de Micr
Page 60 and 61: * Dr. Virginie Jubin (Service de Pn
Page 62: * Pr. Raymond Robert (Groupe d'Etud

Abstract book 2011 - ISHAM

Create successful ePaper yourself

Delete template?

Save as template?