MIU User Guide For Zeiss AxioVert 200 Inverted Fluorescence ...

CONTENTS1. General ................................................................................................................. 31.1 Instrument .................................................................................................................. 31.2 Reservations................................................................................................................ 31.3 Billing .......................................................................................................................... 31.4 Unauthorized use ........................................................................................................ 31.5 Users’ mailing list ........................................................................................................ 41.6 User guide ................................................................................................................... 41.7 Acknowledging MIU .................................................................................................... 41.8 Image processing and analysis ..................................................................................... 42. Microscope use ................................................................................................... 42.1 Settings for the light path ............................................................................................ 42.2 Objectives ................................................................................................................... 62.3 Fluorescence filters ..................................................................................................... 62.4 Transmitted (bright-field) light microscopy .................................................................. 72.4.1 Koehler illumination ............................................................................................. 72.4.2 Phase-contrast imaging ........................................................................................ 82.4.3 DIC imaging .......................................................................................................... 83. Imaging .............................................................................................................. 103.1 Zeiss AxioCam HRc 14-bit color CCD camera .............................................................. 103.2 Zeiss AxioVision software (version 3.1) ...................................................................... 103.2.1 Live view ............................................................................................................ 103.2.2 Adjustment of Camera Settings .......................................................................... 113.3 Display properties ..................................................................................................... 133.3.1 Adding a scale bar .............................................................................................. 144. Saving and transferring images ....................................................................... 154.1 File formats ............................................................................................................... 154.2 Back-up copies .......................................................................................................... 154.3 Saving images ............................................................................................................ 154.4 Saving images in Mcbserver1..................................................................................... 154.5 Saving images into your own computer ..................................................................... 164.6 Saving images into network folder............................................................................. 164.7 Saving images into a USB stick or CD ......................................................................... 164.8 Saving images locally ................................................................................................. 165. Ending your imaging session ........................................................................... 186. Troubleshooting ................................................................................................ 186.1 Microscope (both fluorescence and transmitted light imaging) ................................. 186.1.1 Fluorescence light microscopy ............................................................................ 186.1.2 Transmitted light microscopy ............................................................................. 196.2 Camera ..................................................................................................................... 196.4 AxioVision software ................................................................................................... 202