MIU User Guide For Zeiss AxioVert 200 Inverted Fluorescence ...

1. General1.1 InstrumentThis user guide covers the use of the Zeiss AxioVert 200 inverted epifluorescencemicroscope. For more information on each MIU instrument, visit:http://www.miu.helsinki.fi/instruments/index.htmAn inverted microscope is usually used for observing living cells on culture dishes and bottlesthat do not physically fit under an upright microscope. In an inverted microscope, thecondenser is above the specimen plane and the transmitted light source (halogen bulb) isabove the condenser. In an inverted epifluorescence microscope, there is also a light source(usually a mercury bulb) for reflected light that enables detection of fluorescence signal fromthe specimen.1.2 ReservationsInstructions for Scheduler online reservation system can be found on the MIU web page:http://www.miu.helsinki.fi/reservations.htm. You can make your reservations two weeks inadvance. The minimum time block you can reserve is 15 min. If you need to cancel yourreservation, it must be done before your reservation starts. Unused and uncancelledreservations will also be charged.If you want to extend your session and there are no reservations after you, you can use the"extend" option in Scheduler (click first on your reservation). If your reserved time is alreadyup, you cannot extend your present reservation any more.Make sure you are familiar with the use of Scheduler and MIU user policy and fees(http://www.miu.helsinki.fi/userinfo.htm and http://www.miu.helsinki.fi/fees.htm).1.3 BillingThe billing is based ONLY on the reservations made in Scheduler - the logbook markings donot count. The fee for the one hour training session is 21 € for internal users (July 2008).Check the current rate on the MIU Fees web page. Any time after the initial training, you canask MIU staff to give you additional customized training (charged the same way as the initialtraining).1.4 Unauthorized useMIU periodically checks the logins of the computers. If your logon/off times exceed yourreserved time in Scheduler, MIU will consider that as unauthorized use of the instrumentand will ask for an explanation. For unauthorized use of the instrument, MIU may issue awarning. After three warnings, MIU may revoke the user's license. Also, misuse/neglect ofthe instrument may lead either to a warning or cancellation of the user license, and therepair costs may be charged.3