MIU User Guide For Zeiss AxioVert 200 Inverted Fluorescence ...

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- Select a 10x objective and DIC .3-.4 condenser position by rotating manually thecondenser wheel- Focus on the specimen- Close luminous field diaphragm until its edges canbe seen through oculars (Fig. 6, a wheel in front ofthe top part of the condenser).- Focus the image of diaphragm edges by movingthe condenser up or down (Fig. 6, arrow 2).- Use centering screws to move the diaphragmimage to the center of the field of view (Fig. 6,arrows 3).- Open the luminous field diaphragm until its edgesare outside the field of view (Fig. 6, arrow 1).- Remove one ocular by pulling it out and close theaperture diaphragm until it is about 80% of thediameter of the objective pupil. The aperturediaphragm is adjusted by moving left/right the black level above the condenserposition window in the front of the condenser.When adjusting Koehler illumination for other objectives, it is sufficient just to adjustthe luminous field diaphragm and the aperture diaphragm without taking out theocular.2.4.2 Phase-contrast imagingCheck that is objective is suitable for phase-contrast imaging. Rotate the condenser so thatthe matching condenser position (Ph1 or Ph2) is shown in the front (Fig. 5).2.4.3 DIC imagingTurn the polarizer into to the light path (Fig. 9). The polarizer is a round filter above thecondenser.8
Pull the analyzer located on the left side of the microscope to the left so that it is in positionshown in (Fig. 10).Figure 7The polarizer for DIC imagingFigure 8The analyzer for DIC imagingRotate the condenser to the correct DIC position (Fig. 5). Each DIC position is marked with arange (e.g. ..3-.4). Numerical aperture of the objective must fit into that range.Above the polarizer, there is a slider that is used for adjusting the angle between thepolarizing plane of the polarizer and analyzer. Move the slider while looking at the DICimage.The angle of the DIC-slider is adjusted with the little knob (Fig. 11). It affects the pseudo3Deffectthat the DIC imaging creates.Figure 9The knob for adjusting the angle of the DIC-slider9
Page 1 and 2: MIU User Guide For Zeiss AxioVert20
Page 3 and 4: 1. General1.1 InstrumentThis user g
Page 5 and 6: Figure 12Adjustment of the light pa
Page 7: 2.4 Transmitted (bright-field) ligh
Page 11 and 12: Focusing is done by using the focus
Page 13 and 14: The recommended minimum camera reso
Page 15 and 16: When you click with the right mouse
Page 17 and 18: taken!). There is a 3 GB limit for
Page 19 and 20: The whole image is too bright:Is th

MIU User Guide For Zeiss AxioVert 200 Inverted Fluorescence ...

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