Honey Sugars by HPLC-CAD

The CoronaTMCharged Aerosol DetectorResponse200000150000100000Raffinose (r2 = 0.9998)Melezitose (r2 = 0.9999)Maltose (r2 = 0.9995)Trehalose (r2 = 0.9999)Glucose (r2 = 0.9999)Fructose (r2 = 0.9999)Sample preparationStandards were obtained from Sigma (St. Louis, MO)and dissolved in water (1mg/mL). Further dilutionswere in water. Honey samples were diluted in waterprior to analysis.Conclusions5000000 200 400 600 800 1000Mass (ng on column)Figure 2. Response Curves.The Corona CAD provides universal detection ofnonvolatile analytes with response independent ofchemical properties, a wide dynamic response range,high sensitivity and good precision. Thesecharacteristics, along with reliability and simpleoperation, make this a superior detector for a widerange of HPLC analyses.For more information about this application, theCorona CAD, or charged aerosol detection visitwww.coronacad.com. We are interested in youropinions and are available to answer any questions youmay have: please contact a technical representative at978.250.7082, or if e-mail is more convenient, sendyour questions to coronacad@esainc.com.Ordering informationFigure 3. Analysis of Different Diluted Honey Samples.DescriptionCoronaThermal Organizer ModuleNitrogen generator 70-6003Pump, model 582 70-4050Autosampler, model 542 70-4152Elite software including PC 70-5073Column, Shodex Asahipak, NH2P-50 4EPart Number70-6350 (100/120V)70-6351 (230/240V)70-5499TAESA Biosciences Inc. • 22 Alpha RoadChelmsford, MA 01824-4171U.S.A.Tel: (978) 250-7000Fax: (978) 250-7090www.esainc.comwww.coronacad.comESA Analytical • Brook FarmDorton, AylesburyBuckinghamshireHP18 9NHEnglandTel: 44 (0)1844 239381www.esainc.comESA, Inc. is an ISO 9001 and 13485 Certified CompanyCorona TM and CAD TM are trademarks of ESA, Inc.ESA ®is a registered trademark of ESA, Inc.The Corona CAD is covered by multiple patents70-6977IA-1