Guide to Isolated Heart Perfusion Systems - Harvard Apparatus

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UP-100IH BASIC LANGENDORFFUP-100IH: Core SystemsUP-100IH Core System, Basic:73-4384 (115V) or 73-4385 (230V)UP-100IH Core System, Advanced:73-4386 (115V) or 73-4387 (230V)Includes:• UP-100 Base Unit• Heart Chamber and Holder to UP-100 System• UP-100 Addition for Langendorff (with cannulae forrat/guinea pig)• Thermocirculator• Analog Roller Pump• Compliance Chamber (Windkessel)• Perfusion Pressure Measurements° Pressure Transducer° TAM-D PLUGSYS Transducer Amplifier Module• PLUGSYS Basic System Case (Core System 6 Slot Units)- UP-100IH Basic: PLUGSYS 601 Case,10 Slot Units*- UP-100IH Advanced: PLUGSYS 603 Case,20 Slot Units• Left Ventricular Pressure Measurement° Pressure Transducer° TAM-A PLUGSYS Transducer Amplifier Module* If your chosen options require more than 10 Slot Unitsyou will need to choose the UP-100IH Advanced system.For a working unit, the core system requires theaddition of a selection of one of the optionsUP-MOUSE or UP-RAT/GP.UP-MOUSE 73-4388Full Range ofAortic CannulaePLUGSYS ModularAmplifier SystemAdditions to UP-100IH Core System for mouse heartsIncludes:• Aortic Cannula with Luer Taper for Mice, OD 1.0 mm• Pump Tubing (TYGON)• Mini Balloon Kit for Mouse Heart• Mouse Ventricular Balloon Assembly Kit• 1 Liter Jacketed Buffer Reservoir and Tube SetISOLATED HEART PERFUSION SYSTEMUP-RAT/GP 73-4389Additions to UP-100IH Core System forrat/guinea pig heartsIncludes:• Pump Tubing (TYGON)• 2 Liter Jacketed Buffer Reservoir and Tube Set• Balloon Kit for rat/guinea pig hearts° 10 Balloons, Size 5 (0.1 ml Volume)° Spindle Syringe & Holder for Precise Adjustment ofPreload Pressure° Stainless Steel Balloon Catheter with Ball-Mountemail physiology@harvardapparatus.com web www.hugo-sachs.de • www.harvardapparatus.com 11
ISOLATED HEART PERFUSION SYSTEMUP-100IH: OptionsSCP 73-2806Constant Pressure/Flow ControllerChoose this option if you require the ability to perfuse aheart in constant pressure mode:• Allows operation in constant pressure or constant flowwith simple switch• Additional measured signal/calculated parameters° Indirect Coronary Flow measurement° Calculation of Coronary Resistance• Accurate control of perfusion pressure or flow, evenat very low flow rates•Flexible perfusion circuit setup adjusts to suitindividual perfusion conditionsIncludes:• SCP PLUGSYS Perfusion Controller Module° Utilizes 2 Slot UnitsFiber Oxygenatormounted to topplate of IH-SRUP-FLOW 73-4390Direct coronary flow measurement to UP-100IH formouse, rat or guinea pig hearts• Ultrasonic transit time direct flow measurement –gold standard• Unique integration of flow probe in perfusion path•Laminar flow lines improve accuracy of flow measurement• Thermal properties of Perspex adapter reducetemperature loss•Increase system flexibility by allowing the study ofMyogene Autoregulation (Reactive Hyperemia)• Indirect flow measurement can typically followincreasing flow rates but often lags behinddecreasing flow ratesIncludes:• 1.5 PRB Perivascular Flow Probe• Flow Probe In-line Adapter &Mounting Block• TTFM PLUGSYS Flowmeter° Utilizes 2 Slot Units• Flow Probe Extension CableUP-FIBEROXY 73-4048Fiber OxygenatorOxygenating System for foaming perfusatesChoose this option if you are using buffer supplementedwith albumin, fatty acids, washed erythrocytes, or otherfoaming additive.Features:• MediSulfone Membrane Material• 0.25 m 2 Oxygenating Surface Area• 19 ml Priming VolumeIncludes:• Holder for Oxygenator• Fiber Oxygenators, pkg. of 5• Mounting and Connecting Kits for Oxygenator12 email physiology@harvardapparatus.com web www.hugo-sachs.de • www.harvardapparatus.com
Page 1 and 2: IsolatedISOLATED PERFUSED HEARTHear
Page 3 and 4: INTRODUCTION TO IH-SRTHE LANGENDORF
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Guide to Isolated Heart Perfusion Systems - Harvard Apparatus

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