C - DTU Nanotech - Danmarks Tekniske Universitet

Contents
Nomenclature 9
1 Introduction 11
2 Overview of Small–Scale Cultivation Systems 13
2.1 Shaken small–scale cultivation systems . . . . . . . . . . . . . . . . . . . . . 13
2.2 Stirred small-scale cultivation systems . . . . . . . . . . . . . . . . . . . . . . 14
2.3 Special small–scale cultivation systems . . . . . . . . . . . . . . . . . . . . . 14
3 Theoretical Considerations of Polymers for Bio-Microsystems 17
3.1 PMMA and laser micromachining . . . . . . . . . . . . . . . . . . . . . . . . 17
3.2 Topas R○ and the CNC micromilling machine . . . . . . . . . . . . . . . . . . 19
4 Basic Principles of a Peltier Element 23
5 Freezing Different Samples using Peltier Cooling 27
5.1 Materials and Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.1.1 Fabrication of four different freezing chambers . . . . . . . . . . . . . 27
5.1.2 Freezing experiments with PBS–buffer relating to the freezing chamber
design . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.1.3 Viability experiments with E. coli cells . . . . . . . . . . . . . . . . . 32
5.1.4 Freezing experiments in design II with PBS–buffer and LB–medium
relating to the volume and the temperature . . . . . . . . . . . . . . 34
5.2 Results and discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.2.1 Fabrication of four different freezing chambers . . . . . . . . . . . . . 35
5.2.2 Freezing experiments with PBS–buffer relating to the freezing chamber
design . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.2.3 Experiments with E. coli cells . . . . . . . . . . . . . . . . . . . . . . 37
5.2.4 Freezing experiments in design II with PBS–buffer and LB–medium
relating to the volume and the temperature . . . . . . . . . . . . . . 38
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