Inhibition of lactate dehydrogenase A induces oxidative stress and ...

More documents

Recommendations

Info

Fig. S2. (A and B) FX11 and its derivative E are competitive inhibitors of LDHA with NADH as substrate. Lineweaver–Burk plots were determined from triplicateexperiments using averages of activities. K i determination was performed with 13.5 μM FX11 or 27 μME.(C)Affinity chromatography of LDHA using sepharoseimmobilizedFX11 or E. Equal volumes of P493 human B cell lysates were chromatographed with six column volumes of high salt (1 M NaCl) wash, followed byelution with 1 mM NADH. LDHA activity was determined for each fraction, and the experiment was replicated with a representative experiment shown.Le et al. www.pnas.org/cgi/content/short/09144331074of11
Fig. S3. (A) FX11 and/or FK866 effects on NADPH/NADP + ratio. P493 cells were treated with 10 μM FX11 and/or 0.1 nM FK866 for 24 h before the NADPH/NADP ratio was assayed. FX11 treatment compared with the control (P = 0.8) and FK866 treatment compared with the control (P = 0.9). (B) Effects of FX11treatment on glucose uptake by P493 cells. A fluorescent analogue of 2-deoxyglucose (2-NBDG) was used to assay the glucose uptake of P493 cells, followed byflow cytometry. Viable cells taking up glucose were determined by negative 7-AAD and positive 2-NBDG. The number in each panel indicates the geometricalmean of green fluorescence intensity (520 nm) of the cell population.Le et al. www.pnas.org/cgi/content/short/09144331075of11
Page 1: Inhibition of lactate dehydrogenase
Page 4 and 5: further slowed by FX11 (Fig. S5F).
Page 7: Supporting InformationLe et al. 10.
Page 12 and 13: Fig. S4. (A) Effect of FK866 and FX
Page 14 and 15: Fig. S6. (A and B) Characterization
Page 16 and 17: Fig. S8. (A) Pimonidazole-labeling

Inhibition of lactate dehydrogenase A induces oxidative stress and ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?