Inhibition of lactate dehydrogenase A induces oxidative stress and ...
Inhibition of lactate dehydrogenase A induces oxidative stress and ...
Inhibition of lactate dehydrogenase A induces oxidative stress and ...
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Fig. S4. (A) Effect <strong>of</strong> FK866 <strong>and</strong> FX11 on P493-6 cell proliferation. Live cells were counted using trypan blue dye exclusion. Experiments were done in duplicate,<strong>and</strong> the entire experiment was repeated two times with similar results. The bars represent the averages <strong>of</strong> duplicate samples from one experiment. Thecombination <strong>of</strong> FX11 <strong>and</strong> FK866 doses revealed a CI-50 = 0.78 calculated using R 2.9.1 s<strong>of</strong>tware. (B) FX11 treatment increases cellular NADH fluorescence <strong>of</strong>P493-6 cells compared with control, compound E, or FK866 treatment. The number in the P2 box represents the average percentage <strong>of</strong> cellular NADH aut<strong>of</strong>luorescence(n = 3). FX11 treatment compared with control (P = 0.005), FK866 compared with control (P = 0.144), <strong>and</strong> E treatment compared with control(P = 0.38). (C) Fluorescence emission spectrum <strong>of</strong> NADH (green line), FX11 (red line), or E (blue line). Note that the emission maxima <strong>of</strong> FX11 or E (390 nm) donot coincide with that <strong>of</strong> NADH (460 nm), excluding the direct contribution <strong>of</strong> FX11 to the aut<strong>of</strong>luorescence <strong>of</strong> FX11-treated cells at 460 nm.Le et al. www.pnas.org/cgi/content/short/09144331076<strong>of</strong>11
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