Inhibition of lactate dehydrogenase A induces oxidative stress and ...

Fig. S6. (A and B) Characterization of glucose, glutamine, and pyruvate dependency of different human breast cancer cell lines, MCF-7 and MDA-MB-453.Cells were cultured in media with different availability of glucose, glutamine, and pyruvate. All media were supplemented with 10% (vol/vol) bovine fetalserum and 1% penicillin–streptomycin. Averages of cell numbers from triplicate experiments are shown ± SD. (C) Growth curves for control, electroporated,and siControl cells compared with cells treated with siLDHA. ON-TARGETplus pool was used as siRNAs targeting human LDHA, and Nontargeting Pool was usedas siControl. The siRNAs were transfected into 2 × 10 6 cells at 0 and 24 h by electroporation. (D) Cells were harvested for immunoblot analysis. Rabbitmonoclonal anti-LDHA was used to detect LDHA, and the membrane was reprobed with anti-α-tubulin as a loading control.Le et al. www.pnas.org/cgi/content/short/09144331078of11