Genetics: From Genes to Genomes

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Three experiments elucidate cis and transacting elements using F’ F plasmid.Insert Figure 17.9ahere• Inducible synthesis• lacI + gene encodes adiffusible element thatacts in trans bybinding to anyoperator it encountersregardless ofchromosomal location.Fig. 17.10 aCopyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display17-20
NoninducibleInsert Figure 17.9bhere• All operator sites (O+)eventually occupied bysuperrepressor• lacI superrepressor cannot bind inducer.• lacI s mutant encodes adiffusible element thatbinds to operatorregardless of chromosomallocation (trans actingelement).Fig. 17.10 bCopyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display17-21
Page 1 and 2: Powerpoint to accompanyGenetics: Fr
Page 3: Outline of Chapter 17• There are
Page 6 and 7: Fig. 17.2Copyright © The McGraw-Hi
Page 8 and 9: The utilization of lactose by E. co
Page 10 and 11: The Operon Theory• The players•
Page 12 and 13: The Operon Theory• Induction• L
Page 14 and 15: Experimental evidence for repressor
Page 16 and 17: Inducer releases repressor to trigg
Page 18 and 19: Changes in the operator can alsoaff
Page 22 and 23: ConstitutiveInsert Figure 17.9chere
Page 24 and 25: Some positive regulators increasetr
Page 26 and 27: Many DNA-Binding proteins containa
Page 28: Most regulatory proteins are oligom
Page 31 and 32: Using the lacZ gene as a reporter o
Page 33 and 34: Creating acollection oflacZinsertio
Page 35 and 36: The attenuation of gene expression:
Page 37 and 38: Termination of transcription finetu
Page 39 and 40: Global regulatory mechanisms coordi
Page 41 and 42: What enables transcription of σ 32
Page 43 and 44: Microarrays - a tool for uncovering
Page 45 and 46: Regulation of Virulence Genes inV.
Page 47 and 48: • Isolated a gene that regulates
Page 49: Unanswered Questions Remain• Why

Genetics: From Genes to Genomes

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