Genetics: From Genes to Genomes

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Summary• E. coli’s heat shock response is controlled byalternative sigma factors that recognize differentpromoter sequences.• Alternative sigma factors bind to RNA polymeraseas temperatures change to start transcription ofheat shock proteins.• The induction of alternative sigma factors thatrecognize different promoter sequences serve asglobal control regulatory mechanisms in E. coliand many other bacteria.Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display17-42
Microarrays – a tool for uncoveringchanges in gene expression• Cellular responses to global environmentalchanges can be measured by microarrayanalysis of mRNA isolated from culturesgrown in different environmental conditions.• Comparisons of wild-type cultures withstrains containing mutations in keyregulatory regions help identify genes andregulatory elements involved in response tospecific environmental changes.Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display17-43
Page 1 and 2: Powerpoint to accompanyGenetics: Fr
Page 3: Outline of Chapter 17• There are
Page 6 and 7: Fig. 17.2Copyright © The McGraw-Hi
Page 8 and 9: The utilization of lactose by E. co
Page 10 and 11: The Operon Theory• The players•
Page 12 and 13: The Operon Theory• Induction• L
Page 14 and 15: Experimental evidence for repressor
Page 16 and 17: Inducer releases repressor to trigg
Page 18 and 19: Changes in the operator can alsoaff
Page 20 and 21: Three experiments elucidate cis and
Page 22 and 23: ConstitutiveInsert Figure 17.9chere
Page 24 and 25: Some positive regulators increasetr
Page 26 and 27: Many DNA-Binding proteins containa
Page 28: Most regulatory proteins are oligom
Page 31 and 32: Using the lacZ gene as a reporter o
Page 33 and 34: Creating acollection oflacZinsertio
Page 35 and 36: The attenuation of gene expression:
Page 37 and 38: Termination of transcription finetu
Page 39 and 40: Global regulatory mechanisms coordi
Page 41: What enables transcription of σ 32
Page 45 and 46: Regulation of Virulence Genes inV.
Page 47 and 48: • Isolated a gene that regulates
Page 49: Unanswered Questions Remain• Why

Genetics: From Genes to Genomes

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