Genetics: From Genes to Genomes

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Factors influencing increase in σ 32activity after heat shock• Increase in transcription of the rpoH gene• Increase in the translation of σ 32 mRNAstemming from greater stability of rpoH mRNA• Increase in the stability and activity of the σ 32protein. Chaperones DnaJ/K bind and inhibit σ 32under normal conditions. At high temperature,binding to σ 32 does not occur and more σ 32 is freeto associate with RNA polymerase.• Inactivity of σ 70 decreases competition with σ 32 toform RNA polymerase holoenzyme.Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display17-40
What enables transcription of σ 32during heat shock?• Normal temperatures, rpoH gene (encodes σ 3232 ) haspromoter sequence recognized by σ 70 which startstranscription• High temperatures (no σ 70 ) a different promotersequence of the rpoH gene is recognized by a differentsigma factor, σ 24Figure 17.21 bCopyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display17-41
Page 1 and 2: Powerpoint to accompanyGenetics: Fr
Page 3: Outline of Chapter 17• There are
Page 6 and 7: Fig. 17.2Copyright © The McGraw-Hi
Page 8 and 9: The utilization of lactose by E. co
Page 10 and 11: The Operon Theory• The players•
Page 12 and 13: The Operon Theory• Induction• L
Page 14 and 15: Experimental evidence for repressor
Page 16 and 17: Inducer releases repressor to trigg
Page 18 and 19: Changes in the operator can alsoaff
Page 20 and 21: Three experiments elucidate cis and
Page 22 and 23: ConstitutiveInsert Figure 17.9chere
Page 24 and 25: Some positive regulators increasetr
Page 26 and 27: Many DNA-Binding proteins containa
Page 28: Most regulatory proteins are oligom
Page 31 and 32: Using the lacZ gene as a reporter o
Page 33 and 34: Creating acollection oflacZinsertio
Page 35 and 36: The attenuation of gene expression:
Page 37 and 38: Termination of transcription finetu
Page 39: Global regulatory mechanisms coordi
Page 43 and 44: Microarrays - a tool for uncovering
Page 45 and 46: Regulation of Virulence Genes inV.
Page 47 and 48: • Isolated a gene that regulates
Page 49: Unanswered Questions Remain• Why

Genetics: From Genes to Genomes

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