SCIENTIFIC PAPERS
SCIENTIFIC PAPERS
SCIENTIFIC PAPERS
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Scientific Papers, USAMV Bucharest, Series A, Vol. LII, 2009, ISSN 1222-5339ASSESSMENT OF GENETIC DIVERSITY IN LENTIL (LENS CULINARISMEDIK.) AS REVEALED BY RAPD MARKERSI. ALABBOUD, LIZICA SZILAGYI, GH.V. ROMANUniversity of Agronomic Sciences and Veterinary Medicine of BucharestKeywords: Lens culinaris, RAPD markers, genetic diversityAbstractThe genetic diversity in nine varieties of lentil (Lens culinaris Medik.) - six cultivarsobtained from ICARDA, Syria, one cultivar developed in Romania and two landraces fromGermany and France - was evaluated using RAPD (Random Amplification PolymorphicDNA) markers. Ten primers were assayed and among them only a few (4) have presentedthe polymorphic bands. Genetic distances were calculated using Nei&Li (1979) similaritycoefficient, displayed in a dendrogram (UPGMA method). Cluster analysis based on RAPDamplification products divided genotypes in two main groups according to theirgeographical origin and their maturity. The similarity values found were higher than 70%,suggesting that genetic diversity between lentil genotypes analyzed are relatively low.439