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<strong>Ozean</strong> Journal of Applied Sciences 2(2), 2009Table (2): Production of gladiolus cormlets and florets affected by putrescine, ascorbic acid and thiamine (Mean of the two seasons).TreatmentsNo. ofcormletsF.W ofcormlets(g)D.W ofcormletsSpike length(cm)No. of floretsF.W offloretsD.W offlorets(g)F.W of floretD.W of floretControl 6.33 11.50 1.03 20.70 5.00 16.43 2.05 2.56 0.37Put. 50 ppm 14.33 28.03 2.59 35.00 11.33 31.88 4.25 3.11 0.45Put. 100 ppm 17.00 26.68 2.53 38.10 12.00 31.87 4.42 3.42 0.50Put. 200 ppm 22.00 30.86 2.89 42.50 14.33 33.50 4.77 3.66 0.55Asc. 50 ppm 11.00 17.15 1.57 21.63 7.33 22.78 3.36 2.90 0.42Asc. 100 ppm 12.67 20.45 1.82 25.00 8.67 26.85 3.91 3.21 0.46Asc. 200 ppm 13.33 25.20 2.47 28.03 9.67 32.98 4.72 3.45 0.51Thia. 50 ppm 7.67 13.58 1.25 22.67 6.33 19.55 2.69 2.79 0.39Thia. 100 ppm 8.67 18.95 1.73 27.13 7.66 22.32 3.15 3.40 0.47Thia. 200 ppm 8.33 15.53 1.46 24.43 6.33 22.08 3.11 3.10 0.41LSD 5% 2.15 2.88 0.33 3.71 2.11 2.54 0.49 0.23 0.03Putrescine (Put.), Ascorbic acid (Asc.), Thiamine (Thia)173
<strong>Ozean</strong> Journal of Applied Sciences 2(2), 2009mg/1001.4001.2001.0000.8000.6000.4000.2000.000Chl. A Chl. BChl. A +B CarotenoidsControl 50 100 200 50 100 200 50 100 200Put. Asc. ThiaTreatmentsFig.(1): Effect of Put., Asc. and Thia on photosynthesis pigments of gladiolus plantsTable (3): Effect of foliar application of putrescine, ascorbic acid and thiamine on photosynthetic pigmentsand soluble sugars (mg/g) of gladiolus plants (Mean of the two seasons)TreatmentsCholorophylls (mg/g F.W)Carotenoidschl a chl b a+b mg/g F.WSolublesugarsControl 0.618 0.301 0.919 0.607 2.00Put. 50 ppm 0.642 0.340 0.982 0.739 3.00Put. 100 ppm 0.826 0.425 1.251 1.045 5.00Put. 200 ppm 0.850 0.474 1.324 1.083 5.80Asc. 50 ppm 0.702 0.315 1.017 0.714 2.90Asc. 100 ppm 0.792 0.418 1.210 0.954 4.10Asc. 200 ppm 0.733 0.397 1.130 0.925 4.60Thia. 50 ppm 0.665 0.332 0.997 0.611 2.40Thia. 100 ppm 0.714 0.374 1.088 0.834 3.30Thia. 200 ppm 0.680 0.328 1.008 0.782 3.70LSD 5% 0.018 0.006 0.008 0.005 0.51Putrescine (Put.), Ascorbic acid (Asc.), Thiamine (Thia)The increments were (190.0%, 130.0 and 85.0%) respectively compared with untreated plants in the twoseasons. Moreover, it has been proposed that Polyamines may retard senescence either by inhibitingethylene production (Suttle, 1981) or by stabilizing nucleic acid and cell membrane against enzymaticdegradation and solute loafpage (Mansour and Mutawa, 1999). In addition, Rowland et al., (1988) foundthat Pas.are also part of the overall metabolism of nitrogens compounds. PAs.(i.e. putrescine, spermine andspermidine) are low molecular weight polycations, which are involved in the regulation of growth andstress, propably by binding to negatively charged macromolecules (Messiaen et al. , 1997). Exogenousapplication of Put.to several plant species have been shown to retard chlorophyll loss and senescence (Leeet al. , 1997) and protect plants against environamental stress (Mo and Pua, 2002). Ma et al., (1996)suggest that the effect of PAs. in inhibiting chlorophyll degradation may be related to the inhibition ofperoxidase activity.174
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