ACQUITY UPLC HT Column Heater Instructions - Waters

Disassembling and assembling the in-line filter unit:FlowOutlet assembly Filter Inlet nutTP025436. From the in-line filter unit, remove the inlet nut that contains the usedfilter.Note: Discard the used filter; it cannot be used again.7. Insert a new filter, and then reassemble the in-line filter unit.8. Start the mobile phase flowing.9. Examine the components for leaks, tightening fittings as needed.10. Place the assembled components into the column tray, push down themetal latch to secure the tubing, and tilt the tray upward to the closedposition.Replacing the column stabilizer assemblyPlace wrenches on flats toassemble and disassemble.Requirement: Stop any currently running mobile phase before replacing thecolumn stabilizer assembly.Warning: To prevent burn injuries, set the column temperature to Off,and then allow the column compartment and its components to cool for60 minutes before touching them. Monitor the column compartmentinternal temperature to ensure all components are cool.Caution: To prevent contamination, wear powder-free, non-latexgloves when replacing the column stabilizer assembly.18