Programme Book - UPM - Universiti Putra Malaysia

P22DEEP EUTECTIC SOLVENT AS MEDIA FOR ENZYME-CATALYZED REACTIONSE. Abdulmalek*, N. Abdul Rahman, S. N ZainulAbidin, L.S. Ho, S. ZulkefliDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPMSerdang, Selangor, MalaysiaCorrresponding author: emilia@upm.edu.myDeep eutectic solvent (DES), a new generation of ionic liquidS, compose of a salt anda hydrogen bond donor. Mixing of the two components, sometimes both are solids,depressed the freezing point and result in a liquid. In the describe work, a fewenzyme-catalyzed reactions were performed in DES. Hydrolysis of ethyl butyrate wasperformed in DES that consisted of choline chloride and xylitol (1:1) with Novozyme435 as catalyst at 50°C, able to obtain 78% conversion after 24 h. The hydrolysis ofbutyl levulinate in mixtures of choline chloride and glucose under the same conditionsgave 80% conversion. Separately, DES was applied as treatment media for oil palmtrunk (OPT), an oil palm biomass in DES prior to enzymatic hydrolysis.The OPT washeat treated in choline chloride (ChCl)/glycerol and ethylammonium chloride(EAC)/glycerol mixture for 72 h after which hydrolysis was carried out usingcellulose and β-1,4-glcosidase. The outcome showed that ChCl:glycerol(1:2) DESwas more effective which resulted in a higher glucose yield.37 |16 th Industrial Chemistry Seminar: Chemistry- A Passport to a Brighter Future